Abstract: Plants are often subjected to many abiotic stresses. Temperature and high salt are one of the important abiotic factors affecting plant growth and development. Therefore, it is particularly important to study the molecular mechanism of plant response to stress. In this study, 7 members of KcHTR gene family were screened and cloned from the high-temperature transcriptome data of desert plant Karelinia caspica. By constructing prokaryotic expression vectors and inducing expression, it was clear that the length of their cDNA was 708 ~ 789 bp, the molecular weight of the expressed protein was 27 ~ 29 kD, and the encoding gene was consistent with the size of the protein. Through experimental exploration, it was found that under the optimal growth temperature of 37℃, the optimal expression system of this family protein was OD600 0.8, the induction concentration of IPTG was 0.5 mmol/L, and the induction time was 8 ~ 10 h. Through simulated high temperature, low temperature and salt stress treatment of recombinant E.coli, the results showed that KcHTRs could enhance the host tolerance to high temperature, low temperature and high salt to a certain extent, which was consistent with the results of abiotic stress expression pattern analysis of KcHTRs in this study. It provides the research basis and basis for further verification of gene function and mining and utilization of desert plant stress-resistance related gene resources.