Abstract:【Objective】 Most species of phoebe are similar in morphology and the boundaries among species are blurred, making the identification and recognition of species very difficult, particularly in the period without flowers and fruits. Micromorphological characteristics of leaves are valuable for the interspecific definition of plants, but studies on Phoebe in this aspect are still scarce. 【Methods】 In this study, authors observed leaf morphology and leaf micromorphology of eight species and one variant of Phoebe in Hubei Province using stereo microscope and light microscope. For all species, authors measured and analyzed the quality properties and quantity properties of stomata and trichomes. The purpose is to provide the necessary basis for the identification and classification of Phoebe. 【Results】 Four types of leaf shapes were characterized, including large, medium, small, and irregular leaves. Trichomes were grouped into thin pubescent, thick pubescent, villous, and mixed pubescent and villous (villous trichomes are mainly found on leaf veins). The trichoid density of P. hunanensis (200 roots/mm2) differed significantly to P. bournei, P. chekiangensis, and P. sheareri (under 100 root/mm2). Shapes of epidermis cells were polygon or irregular, and the anticlinal wall in epidermis cells was classified intostraight-curved, sinuolate or undulated. The shape of stomatas was wide oval or nearly round. Density of stomatal apparatus was highest in P. sheareri(with an average of 700/mm2), and was lowest in P. chinensis and P. hunanensis (less than 500/mm2). In addition, there was a significant negative correlation between density and aspect ratio of stoma, as well as density and length of trichome. 【Conclusion】 It is suggested that the combination of morphological and micromorphological characteristics of leaves could not only provide important basis for identification and differentiation of the nine species, but also provide necessary references for interspecific definition of phoebe.