Abstract:【Objective】 NAC transcription factor is a plant-specific transcription factor family, which is involved in plant biotic and abiotic stress response and hormone signal transduction. 【Methods】 In this study, two NAC transcription factor genes CsNAC79 and CsNAC9 were cloned from tea cultivar ''Longjing 43''. The analysis showed that CsNAC79 and CsNAC9 transcription factors contained typical NAM conserved domains of NAC family members at 15-150 amino acid sites and 11-134 amino acid sites, respectively. Phylogenetic tree analysis showed that CsNAC79 was closely related to Actinidia chinensis, Diospyros kaki and Cynara scolymus; CsNAC9 was closely related to Amygdalus persica, Litchi chinensis and Durio zibethinus. Amino acid hydrophilicity / hydrophobicity analysis showed that both NAC transcription factors were hydrophilic proteins without signal peptides and transmembrane structures. Prediction analysis of cis-acting elements in the promoter region showed that both NAC transcription factors contained abiotic stress response elements. Protein spatial structure analysis showed that CsNAC79 and CsNAC9 proteins were mainly composed of random coils and alpha helices. The expression levels of CsNAC79 and CsNAC9 genes under different abiotic stresses were detected and analyzed by RT-qPCR. The results showed that the expression levels of CsNAC79 and CsNAC9 genes was up-regulated by drought, high salt, high temperature, low temperature, exogenous ABA and exogenous GA3 treatments, indicating that CsNAC79 and CsNAC9 genes were related to abiotic stress resistance response. In summary, CsNAC79 and CsNAC9 genes in tea plants may respond to various abiotic stresses. This study provides a reference for further analysis of the biological functions of CsNAC79 and CsNAC9 transcription factors in the future.