In order to clarify the role of LEA gene in abiotic stress, this study identified the whole genome of the LEA gene family by bioinformatics, and analyzed its expression under different abiotic stress by qRT-PCR. The results indicated that: (1) The presence of 57 members of the LEA gene family that encoded proteins with molecular weights ranging between 6.95-104.98 kD and amino acid lengths between 62-945 aa. (2) The phylogenetic analysis classified the PtrLEA genes into eight subfamilies. LEA_2 had the most members, followed by LEA_1-LEA_6, dehydrin, and SMP. (3) Cis-acting element analysis demonstrated that the PtrLEA promoter contained a significant number of hormone, stress response, and growth and development-related response elements. (4) Transcriptome data analysis indicated tissue-specific expression patterns of the LEA gene family, with PtrLEA15, PtrLEA17, PtrLEA23, and PtrLEA51 highly expressed in all tissues, while three genes did not express in any tissues. (5) The real-time fluorescence quantitative PCR test revealed up-regulated PtrLEA gene expression when exposed to low temperature, drought, and high salt stress conditions, with 6, 2, and 6 genes up-regulated, respectively, suggesting the involvement of this gene family in the response to various non-biological stresses.