Abstract: Based on field investigation and compilation of the list of stone pine and fern resources of Tingjiangyuan National Nature Reserve in Fujian, the flora composition of the area was statistically analyzed by using the principle of flora geography, and compared with the flora of the four neighboring protected areas. The results showed that: (1) There were 129 species of lycopodians and ferns in 23 families, 60 genera, including 3 rare and endangered species. There were 3 families, with a total of 61 species in 23 genera, accounting for 47.29% of the total species. There is 1 genus, a total of 11 species, accounting for 8.53% of the total number of species, and the composition of families and genera is obvious. Monofamilies and oligofamilies accounted for 65.21% of the total family, and monospecies and oligogenera accounted for 90.00% of the total number of genera. (2) In terms of distribution area, tropical components accounted for 100% of the total number of families; Tropical components accounted for 84.21% of the total genus; Tropical component species accounted for 52.85%, temperate component species accounted for 35.25%, mainly tropical Asian distribution species, followed by East Asia and its variants. There are 15 species endemic to China. The tropical attributes of this area are predominant, the tropical relatives are obvious, and there are certain temperate properties. (3) The comparison with the geographical composition of the flora of neighboring protected areas showed that in terms of similarity coefficients of families, genera and species, Tingjiangyuan and the four protected areas reached more than 85%, 74% and 50%, respectively. The germline differentiation degree of 4.75 in this area is similar to that of Liangyeshan 4.69, which is relatively low. In terms of geographical affinity, the stone pine and fern fauna in this area have the highest connection with Liangye Mountain, and the closest kinship is the same, and the kinship relationship with Matou Mountain in Jiangxi is relatively distant.