[Objective] The role of WRKY70 homologous gene in strawberry response to stress was analyzed in depth, so as to provide new genetic resources for strawberry molecular breeding. [Methods] The WRKY70 was cloned from strawberry fruits and leaves by homologous cloning method, and the physicochemical properties, protein structure and evolutionary relationship of the gene were analyzed by bioinformatics analysis, and its expression pattern was analyzed by qRT-PCR data. [Results] The FaWRKY70 was 1 020 bp in length and encoded 339 amino acids. Among the homologous genes obtained, FaWRKY70 had a high degree of amino acid sequence similarity with the same family species such as apple and peony, and most of the homologous genes were related to plant responses to biotic and abiotic stresses, suggesting that FaWRKY70 may be involved in the process of strawberry resistance to stress. FaWRKY70 was expressed in flowers, fruits, leaves, roots and stems of strawberry, with the highest expression level in flowers and the lowest in fruits. After salicylic acid treatment, the expression level of FaWRKY70 could respond rapidly, reaching the highest level after 3 hours, and then gradually decreasing. However, when treated with methyl jasmonate, the FaWRKY70 was less responsive to induction, and the overall trend was down-regulated. [Conclusion] FaWRKY70 can participate in strawberry life activities and hormone signal transduction through different response modes.