【Objective】 Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) plays an important role in ABA signaling transduction and abiotic stress responses. The aims were to further elucidate the regulatory mechanism of SnRK2 gene family members under drought stress in Saccharum spontaneum. 【Methods】 This study identified SnRK2 genes in S. spontaneum based on whole genome data, and conducted bioinformatics analysis and expression analysis under drought stress on them. 【Results】 The results showed that 11 SnRK2 gene family members were identified and named as SsSnRK2.1 to SsSnRK2.11, which were unevenly distributed on 8 chromosomes. The results of physicochemical property analysis showed that the encoded amino acid residues of SsSnRK2s ranged from 227 to 580, the molecular weight ranged from 25 683.53 D to 64 695.8 D, the isoelectric point ranged from 4.62 to 8.94, and all were hydrophilic proteins. The 11 SsSnRK2s could be divided into 3 subgroups, and the conserved motif sequences within the same subgroup were quite similar, with the number of exons mainly ranging from 7 to 9. Cis-acting element analysis showed that the promoters of SsSnRK2 gene family members contained various hormone-related and abiotic stress response cis-acting elements. The analysis of transcriptome data from different tissues showed that SsSnRK2s were expressed in a tissue-specific manner. The qRT-PCR results showed that all SsSnRK2 genes were able to respond to drought stress to varying degrees. 【Conclusion】 The study suggests that the SsSnRK2 genes may play a significant role in the response process to drought stress in S. spontaneum L.