[Objective] The woody stems of plants are difficult to obtain satisfactory microscopic image in both freehand and paraffin sections due to the high content of secondary vascular tissue, high degree of lignification of cell wall. Microtome cryostat can section the tissue with a certain hardness. It will be one of the effective tools for the section of plant woody stems. [Methods] Some woody stems with uniform texture and high water content can be directly sectioning without chemical fixation by using cryo-sectioning. For stems with hard wood and less water content, after fixation with FAA and other fixation, OCT embedding, (-30)-(-25) ℃ section, and staining with 0.1% toluidine blue staining solution. [Results] The microstructure of the wood stem is relatively complete, the image is clear and rich in color. [Conclusion] The microtome cryostat can be used for microstructure sections of wood stem, combined with toluidine blue staining, which has the advantages of simplicity, efficiency and ease of operation.