【Objective】 In order to enrich the data for the study of micropyle closure in gymnosperms and to provide a reference for the phylogenetic study of gymnosperms, the micropyle closure of Platicladus orientalis Franco was investigated.【Methods】 Female cones of Platycladus orientalis were sampled and examined using morphological techniques, including paraffin sections and scanning electron microscopy. Furthermore, cluster analyses based on the mode of micropyle closure were also conducted for Bennettitales.【Results】 (1) After the pollen of Platicladus orientalis enters the ovule, the innermost two cell layers of the integument begin to grow radially inward until the micropyle is completely closed. (2) During the micropyle closure of Platicladus orientalis, the innermost cells of the integument are elongated and conical in shape, with the cell layers typically increasing to around 4 to 6 layers. (3) The micropyle closure of gymnosperms can be divided into four clades. These groups include one for Cycas and Ginkgo, another for Pinus and Podocarpaceae, a separate group for Bennettitales, and a cluster consist of Platycladus orientalis and other species. 【Conclusion】 The micropyle closure of Platicladus orientalis is differ with Ginkgo and cycads. while is similar with Pinus and Bennettitales. This study suggested that evolutionary relationship in micropyle closure between Bennettitales and extant conifers, providing novel morphological evidence for understanding the evolution of gymnosperm reproductive systems.