[Objectives] plant E3 ligases family has been recognized for its involvement in regulating plant responses to low temperature stress，its role in bog bilberry under low temperature stress remains largely unexplored. In this study，we cloned VuARI2 gene，transformation model plants Arabidopsis thaliana to explore the function of VuARI2 gene in responses to low temperature stress. [Methods] VuARI2 gene was cloned from stem of bog bilberry by RACE and RT-PCR and conducted a comprehensive bioinformatics analysis. Expression of VuARI2 in different tissues and stems and flower buds after cold acclimation were analyzed by qRT-PCR. Furthermore，Heterologously express the VuARI2 in Arabidopsi Thaliana，and the transgenic plants were analyzed for the expression levels of VuARI2，physiological indexes，and freezing resistance after cold acclimation. [Results] VuARI2 was successfully cloned，and its open reading frame of 1770 bp，which encoded 589 amino acids. The multi-sequence alignment and phylogenetic tree analysis of VuARI2 amino acids indicated that VuARI2 protein was closely related to ARI2 protein in wild camellia，coffee tree，grape and riverside grape. RT qPCR showed that the expression of VuARI2 in leaves and flower buds of bog bilberry was significantly higher than that in roots and stems，and the expression of VuARI2 in stems and flower buds was significantly induced by low temperature. After cold acclimation，expression levels of VuARI2 gene in transgenic plants was significantly higher in wild type，chlorophyll content decreased significantly lower than that of the wild type；proline content and soluble sugar content were significantly higher than that of the wild type；superoxide dismutase activity；peroxidase activity and catalase activity were significantly higher than that of the wild type；while relative conductivity and malondialdehyde content were significantly lower than that of the wild type. After cold acclimation，the survival rate of transgenic plants was significantly higher than that of the wild type，and the relative conductivity was significantly lower than that of the wild type at freezing temperature，significantly enhance the freezing resistance of plants. [Conclusions] Cloning and obtaining VuARI2-overexpressed A. thaliana plants，functional analysis showed that overexpress of VuARI2 gene could significantly enhance the cold resistance of plants.