【Objective】 The Tonoplast sugar transporter (TST or TMT) is a crucial sugar transport protein in the developmental trajectory of plants. The study aims to investigate the role of this gene family in the progression of grape growth and development, further provide a solid foundation for elucidation of the function of TST gene. 【Methods】 A total of 13 TST genes were identified from the grape genome using homology analysis technique. Information regarding the gene structure and encoded protein was analyzed using bioinformatics tools. Utilizing the qRT-PCR technique, the VvTST expression level in different grape tissues during ''Yinhong'' grape development was analyzed, and a correlation with soluble sugar content in the grapes at different time points were studied. 【Results】 The study''s results indicate that the gene family is distributed across six chromosomes and contains three segmental repetitions and three tandem repetition genes; according to systematics, it can be classified into three subfamilies, each with members sharing structural similarity; cis regulatory elements suggest that TST genes include ample cis regulatory elements related to phytohormone, light, and stress response; the protein structure reveals that the family is composed of α-helices and irregular coils, and the protein model of each subfamily is similar. The qRT-PCR results indicate that VvTST is expressed in various tissues, possessing spatiotemporal expression specificity. Correlation analysis between changes in gene expression levels in grapes and changes in soluble sugar content in grapes found that theexpression levels of four VvTSTs (VIT_18s0001g12560, VIT_18s0122g00850, VIT_04s0023g01860, and VIT_03s0038g03940) exhibit similar trends with the accumulation of soluble sugars in the grape flesh. 【Conclusion】The aforementioned findings indicate that VvTST may play a vital role in the accumulation of soluble sugars in the flesh of grapes.