CHEN Jing~ , CHEN Qi-lin~ , WENG Jun~ , LIU Yuan~ , CHENG Zhi-hui~ , XU Chun-he~
2004, 24(10).
Abstract:Anthocyanidin accumulation in tomato seedling leaves exposed to different R/FR ratio light condition was studied.Spectrophotometric analysis suggested that tomato seedling leaves grown in high R/FR (1.5) condition could normally synthesize anthocyanidin,w
2004, 24(10).
Abstract:磷是能量代谢、核酸以及许多生物膜合成的重要底物。在光合作用、呼吸作用等过程中发挥了重要作用。中国大多数小麦产区的土壤存在着缺磷的问题。磷饥饿给小麦生产造成了很大损失。培育耐低磷小麦是解决这一问题的一个重要途径。在磷饥饿的过程中,哪些基因的表达发生了变化.它们是如何变化的,弄清楚这些问题对于培育转基因耐低磷小麦具有重要的意义。磷转运蛋白基因在植物吸收磷的过程中发挥着重要作用。利用RT—PCR的方法,我们从普通小麦“小偃54”中分离了两个磷转运蛋白基因TaPT8和TaPHT2;1。通过与酵母突变体互补分析表明这两个基因都能够与磷吸收功能存在缺陷的酵母突变体实现功能互补,在低磷条件下有促进酵母突变体吸收磷的作用。进一步分析表明TaPT8属于Pht1家族。TaPHT2;1属于Pht2家族。运用RQRT—PCR的方法进行分析后发现TaPT8在根中表达,受磷饥饿的诱导;TaPHT2;1主要在绿色组织中表达,受磷饥饿的抑制,受光的诱导。TaPT8可能主要参与了小麦的根从土壤中吸收磷的过程。TaPHT2;1可能在磷从细胞质向叶绿体内转运的过程中发挥了重要作用。
2004, 24(10).
Abstract:研究了不同灌水次数及等氮量不同施氮方式对优质强筋小麦品种郑麦9023旗叶净光合速率(Pn)、叶绿素荧光参数等光合性能及产量的影响。结果表明,旗叶Pn、PSⅡ的潜在活性(Fv/Fo)、PSⅡ的光化学最大效率(Fv/Fm)、光化学猝灭系数(qP)、非光化学猝灭系数(qN)及产量在不同灌水次数间的差异均达到显著或极显著水平,其中不灌水处理与灌水处理间的差异较大;在不同施氮处理问,氮肥全部基施与返青期追施(占40%)处理间植株旗叶的Pn、Fv/Fo、Fv/Fm、qP和qN差异不大,但两处理的上述光合参数及产量均明显低于拔节期或孕穗期追施(40%)处理,表明拔节期或孕穗期追施氮肥有助于减缓旗叶光合功能的衰退,延长叶片功能期,提高植株的光合产物积累。研究结果还表明,水、氮运筹对Pn、叶绿素荧光参数及产量性状的调控存在显著的互作效应,其中以灌拔节水 孕穗水并于孕穗期追施氮肥(W2N4)处理组合表现最好。
2004, 24(10).
Abstract:在陕北黄土丘陵沟壑区,对阳坡、半阳坡的不同林龄狼牙刺的生长特征作了系统研究,调查分析了狼牙刺的株高、冠幅、树龄、物种组成、新枝生长量以及地上部生物量和叶量(以柠条为研究对照)等生长特征指标。结果表明:天然灌木狼牙刺的株高在1.50m左右,冠幅约2.0m×2.0m,平均分枝数约4.45。17龄、14龄、6龄狼牙刺地上部生物量和种群密度分别为21.0t/hm2,10000株/hm2;27.3t/hm2,4375株/hm2;6.8t/hm2,8750株/hm2;6、7、8三个月份是狼牙刺新枝的快速生长期,新枝年生长量大小顺序为14龄狼牙刺>6龄狼牙刺>17龄狼牙刺。流域内,狼牙刺个体地径基本保持直线生长,其种群处于旺盛生长阶段。林龄是造成狼牙刺地上部生物量差异的主要内因,而立地条件(土壤水分状况)的优劣以及种群密度则是影响狼牙刺地上部生物量及生长速率的重要外部因素。与柠条相比,狼牙刺在长期的生长发育过程中与土壤水分环境形成了更为协调的关系,表现出较高的土壤水分含量和多年地上部累积生物量,生长旺期盖度均能达到50%以上,应该成为当地造林树种的优先选择之一。
ZHOU Hua-kun , ZHOU Li , ZHAO Xin-quan , LIU Wei , LI Ying-nian , ZHAO Liang
2004, 24(10).
Abstract:The characteristics of clonal plant, Halerpestes cymbalaris were studied in alpine wetland at Haibei Station by investigation and statistics in this paper. The results are as followed: H. cymbalaris with one stolon is the most one and its proportion i
WANG Chang-ting , LONG Rui-jun~ , DING Lu-ming
2004, 24(10).
Abstract:The characteristics of clonal growth of perennial plant Ligularia virgaurea was studied in respect to four different elevation gradients. The results showed: (1) Spacer length was affects by resource levels(soil nutrition), disturbance and community cha
HE Jun-min , SHE Xiao-ping , WANG Rui-bin , LIU Cheng
2004, 24(10).
Abstract:研究了0.4w·m-2的紫外线-B(UV-B)辐射对0.8%NaCl胁迫下冬小麦"小偃926"(TriticumaestivumL.xiaoyan-926)幼苗的生长、光合作用、水分状况、黄酮含量和膜脂过氧化等几方面的影响。结果表明,UV-B辐射和NaCl胁迫单独或复合处理下小麦幼苗的株高、生物量、含水量、净光合速率、蒸腾速率、气孔导度和光合色素含量均显著降低,膜脂过氧化产物丙二醛含量和叶片相对电导率则升高,但NaCl胁迫条件下增加UV-B辐射(复合处理)与单独NaCl胁迫相比,上述指标多数均无显著差异(除复合处理下类胡萝卜素含量显著降低外)。两胁迫因子单独或复合处理均明显提高了小麦幼苗黄酮含量及三种抗氧化酶(SOD、POD和CAT)活性,且复合处理的促进效应最大。脯氨酸含量在单独UV-B辐射下明显降低,在单独NaCl胁迫和复合处理下显著升高,且复合处理下增幅最大。结果说明UV-B辐射不会明显加剧NaCl胁迫下小麦幼苗的伤害,这可能与NaCl胁迫提高了小麦幼苗黄酮含量、脯氨酸含量和抗氧化酶活性有关。
米海莉 , 许兴 , 李树华 , 何军 , 张源沛 , 赵天成 , 马有明
2004, 24(10).
Abstract:在宁夏灵洲区牛心朴子、甘草自然生境条件下,采用土壤控水措施,研究了土壤水分胁迫对两种优势旱生植物牛心朴子(Cynanchumkomarovii)和甘草(Glycyrrhizauralensis)叶片中的叶绿素;根、茎、叶中的可溶性糖、淀粉含量和C/N比值的影响。结果表明:1土壤水分长期胁迫,导致了这两种旱生植物叶绿素a(Chla)、叶绿素b(Chlb)、类胡萝卜素(Car)含量及Chla/Chlb、Car/Chl比值等的显著变化(P<0.01);2甘草Chla的含量和Chla/Chlb的比值始终大于牛心朴子,相反牛心朴子Chlb、Car的含量和Car/Chl比值却又始终大于甘草;3土壤水分长期胁迫引起了两种植物根、茎、叶可溶性糖含量的升高和淀粉含量的降低;4土壤水分长期胁迫引起了两种植物根、茎、叶C/N比值的降低,但牛心朴子C/N比值在处理的各个时期均大于甘草;5土壤水分长期胁迫引起牛心朴子和甘草Chla、Chlb、Car含量及Chla/Chlb、Car/Chl的比值、可溶性糖、淀粉和C/N比值的增加或减少的程度不同。
2004, 24(10).
Abstract:Using four local species of Loess Plateau as experiment materials and pot culture experiments simulating different soil water status, this paper mainly deals with effect of soil water stress on water metabolism and osmotic adjustment substance of differen
2004, 24(10).
Abstract:本实验研究了不同温度(15℃~35℃)对青蒿毛状根生长和青蒿素生物合成的影响,发现25℃有利于毛状根生长,30℃促进了青蒿素生物合成。通过温度改变的二步培养技术(培养前20d温度控制在25℃,后10d温度提高到30℃),青蒿素的产量得到明显提高,高于在恒温培养时(25℃或30℃)的结果。
HU Jing-jiang , WEN Jian-lei , WANG Shu-qing
2004, 24(10).
Abstract:采用盆栽控水法和P—V技术研究分析了不同土壤干旱(速度、程度)条件对元宝枫渗透调节能力的影响。结果表明,元宝枫具有很强的渗透调节能力.但该能力受土壤干旱的速度和程度影响,在缓慢干旱条件下,元宝枫叶片的ψw、ψ0、π100、RWC^0、ROWC^0均明显降低.其中与渗透调节能力直接相关的(π100可下调0.52MPa,ψ0下调1.51MPa。在快速干旱条件下π100和ψ0分别仅下凋0.20MPa和0.48MPa。△π100值也表明缓慢干旱条件下元宝枫渗透调节能力是快速干旱下的45倍。在缓慢干旱条件下.由轻度到中度干旱时其渗透调节能力显著增强(增加270%);由中度到严重干旱时.增加不明显(增加了24.5%)。从3种有机渗透调节物质含量与△π100值的动态变化可见,可溶性糖含量增加对渗透调节能力的贡献是第一位的.其次是Pro、游离氨基酸。
SUN Jian-hua , WANG Yan-rong , YU Ling
2004, 24(10).
Abstract:连续4年对中国28个已通过审定的紫花苜蓿品种的产量及质量性状进行了研究。生育期测定结果表明:达初花期所需天数最少的品种有甘农3号、武功、蔚县、新牧1号、新牧2号、公农2号、甘农1号、准格尔、陇东、无棣和晋南。产量性状分析表明:总干物质产量最高和较高的品种为新疆大叶(35955.6kg/hm^2)、武功、甘农3号、公农2号、新牧2号、新牧1号、肇东、图牧2号、草原2号、龙牧801和龙牧803共11个品种(31088.3~33183.4kg/hm^2);叶茎比最高的品种为龙牧801和无棣。依据对4年总干物质产量、3龄总干物质产量、4年平均日增高、4龄叶面积、4龄总枝条数和叶茎比6个产量性状测定值的不加权平均进行生产性能的聚类分析,认为可将28个品种分为极强、较强、中等、较差和差5个类群。极强类群苜蓿生产能力最强,特征为生长迅速,干物质产量、日增高、枝条数均为最高,叶面积最高或较低;叶茎比为中等;达出花期所需天数为少或多。
2004, 24(10).
Abstract:In order to study the possibility of utilizing oxalate oxidase gene ( OxO ) to increase plant's resistance, the leaf discs from Nicotiana tabacum line 97131 were transformed by using Agrobacterium tumefaciens harbouring the plasmid containing OxO ge
2004, 24(10).
Abstract:The alfalfa mosaic virus coat protein gene was successfully introduced into red clover ( Trifolium pratense L.) plants through their cotyledons, mediated by Agrobacterium tumefaciens . Transgenic plants resistant to kanamycin were obtained after selecti
WANG Li-jun~ , FAN San-hong~ , GUO Ai-guang~
2004, 24(10).
Abstract:通过PCR扩增,从拟南芥中克隆出ats1A基因启动子(包括叶绿体转运肽),将此启动子与GUS基因相连构建植物瞬时表达载体,用基因枪法将之导入烟草进行瞬时表达。GUS基因检测分析表明,ats1A基因启动子能特异的启动GUS基因在烟草叶片中高效表达。
2004, 24(10).
Abstract:采用PCR技术和聚丙烯酰胺凝胶电泳法对6个中国梨品种的s基因型进行了鉴定研究,并与已知S基因型的日本梨品种进行了比较。研究结果表明,供试的6个中国梨品种S基因型均不相同,‘西子绿’、‘金花’和‘金水酥’各包含了S1~S7以外的新的S基因,为这些品种田间授粉品种的选配提供了参考。
YUAN Zong-ying , WU Ying-peng , LI Yan-fang
2004, 24(10).
Abstract:Isolates of Puccinia recondita f.sp. tritici sampling from different area of Shanxi were inoculated on wheat contained the known resistant genes of near-isogenic lines(or monogenic lines) of leaf rust and their virulence frequencies were determined. A
2004, 24(10).
Abstract:根据野外调查和观测,对子午岭次生林区的灌木和森林植被地上部各部分生物量中有机碳的储量进行了比较和分析。在灌木群落中,狼牙刺、虎榛子和沙棘群落中的茎杆与枝条中有机碳的储量分别占地上部生物量中有机碳储量的70.59%~75.33%、95%和83.84%;茎杆和枝条及叶片生物量中有机碳储量的顺序为沙棘>狼牙刺>虎榛子;灌木群落中草本层生物中有机碳储量的顺序为狼牙刺>沙棘>虎榛子;地被层有机物质中有机碳储量的顺序为沙棘>虎榛子>狼牙刺。狼牙刺群落、虎榛子群落和沙棘群落中有机碳的储量分别为15.06~16.25t/hm2、8.84t/hm2和25.67t/hm2。在森林植被中,山杨-辽东栎混交林、山杨林、白桦林、辽东栎林等落叶阔叶林的茎与枝条中有机碳的储量,占地上生物量中总储碳量的88.66%~97.75%、侧柏林和人工油松林针叶林的茎和枝条中有机碳的储量,约占地上生物量中总储碳量的83%,除过人工油松林外,乔木林中灌木层中有机碳储量的顺序为白桦>山杨-辽东栎>山杨>侧柏>辽东栎,乔木林中草本层生物量中有机碳的储量为0.5~0.6t/hm2,人工油松林的草本层生物量中有机碳的储量为1.26t/hm2,天然乔木林下地被物中有机碳的储量,约占群落地上部总有机碳储量的7%~13%。山杨-辽东栎、白桦、山杨、辽东栎、侧柏及人工油松林中有机碳的储
2004, 24(10).
Abstract:The paper chooses Quercus aliena var. acuteserrata community as research material which lies on north aspect of western Qinling mountain.We make conclusions through plenty of wild investigations and inside analysis as the follow: (1) The average specie
ZHANG Wei-guo~ , JIANG Xiao-lei~ , WANG Shu-mao~ , YANG Zhen-yu~
2004, 24(10).
Abstract:A three-year study was conducted in a degraded pasture caused by over-grazing to investigate the effects of mound-building activity of zokor and grazing-prohibited regimes (non-grazed) on the productivity of vegetation community. The results indicated tha
MIAO Li-yun , WANG Xiao-an~ , WANG Zhi-gao
2004, 24(10).
Abstract:The relationships between species diversity indices of Larix chinensis communities and environmental factors were studied quantitatively in this paper. The results were as follows:(1) The species richness and PIE of the communities were negatively relat
2004, 24(10).
Abstract:Based on the statistics and analysis of areal-types of families, genera , species in the flora in Si'er Nature Reserve of Sichuan Province, the floristic characteristic indicates as follow: (1)Components are complex and taxa are abundant.(2)Dominant famil
YU Long-jiang , SUN You-ping , CHENG Hua , ZENG Xian-dong , JIN Wen-wen
2004, 24(10).
Abstract:建立了15种中国独荇菜属植物和秘鲁原产的玛咖的性状矩阵,并采用SPSSV11.0软件建立了树谱图,以此检验《中国植物志》有关该属植物形态学分类的正确性,同时确立玛咖与该属其他植物之间的相互亲缘关系,为研究玛咖的育种、栽培提供理论依据。
2004, 24(10).
Abstract:The morphological variations among five populations of Calligonum rubicundum Bge. from northwestern desert area of China were investigated in the present paper. Univariate and multivariate analyses of morphological characteristics were compared using th
LI Yan , WANG Dong-mei , JIANG Zai-min , TANG Rui
2004, 24(10).
Abstract:以MS、LS、B5、N6、H、Nitsch、White、1/2MS为基本培养基,分别添加0.5mg/L NAA和0.5mg/L BA,分析不同类型培养基对杜仲愈伤组织生长及次生代谢产物含量的影响,并以B5培养基进行光照条件、碳源、蔗糖浓度试验。结果表明:B5培养基不仅有利于愈伤组织生长,也有利于总黄酮的形成,而1/2MS培养基有利于绿原酸的积累;12h/d光照对愈伤组织的生长及绿原酸和总黄酮的合成有明显的促进作用,黑暗不影响愈伤组织的生长,但却抑制绿原酸和总黄酮的形成;3种碳源中,愈伤组织的增长量、绿原酸和总黄酮的含量均以蔗糖为碳源时最高,葡萄糖最低;蔗糖浓度在10~50g/L范围内绿原酸的含量随着糖浓度的升高而升高,40g/L时愈伤组织的增长量和总黄酮的含量最高。
HUANG Ya-fen , YU Long-Jiang~ , LAN Wen-zhi , YANG Xi
2004, 24(10).
Abstract:The role of lipoxygenase (LOX) in the elicitor induced taxol production was investigated. Fungal elicitor treatment effectively increased the LOX activity and taxol production and the LOX inhibitor phenidone could inhibit the induced LOX activity and taxo
WANG Hua-hong~ , GUO Wu-bao~ , GU Ying~ , WANG Zhe-zhi~
2004, 24(10).
Abstract:Fingerprints of Macrocarpium officinale was established by high performance liquid chromatography (HPLC) in the study. Gradient mobile system was applied with acetonitrile - phosphoric acid and the methodological experiments showed high precision and g
DING Chen-xu~ , CHEN Chang-xiang~ , JI Lan-ju~ , JI Wen-he~ , MA Yu-hua~ , CHEN Zhuan-ke~
2004, 24(10).
Abstract:The constituents of essential oil from Elsholtzia ianthina were analysed.The essential oil was extracted with steam distillation. Forty-five compounds were elucidated by means of GC-MS technique. The main constituents in essential oil are tricyclo [4.3.
ZHENG Xu-dong~ , HU Hao-bin~ , CHEN Hao~ , ZHENG Shang-zhen~ , SHEN Xu-wei~
2004, 24(10).
Abstract:从顶羽菊(Centaurea repens L.)中分离得到10个化合物,经光谱分析、化学方法及与已知化合物对照,鉴定它们分别为:三十四碳酸(1)、豆甾—7—烯—3—醇(2)、β—谷甾醇(3)、5—羟基—6,7—二甲氧基黄酮(4)、5—羟基—6—甲基7—O—α—D—半乳吡喃糖双氧黄酮苷(5)、2,4—二(邻甲基偶氮苯)—萘酚—1(6)、β—谷甾醇—β—D—葡萄糖苷(7)、洋芹素—5—O—β—D—葡萄糖苷(8)、洋芹素(9)、山奈素—3—O—β—D—葡萄糖苷(10)。其中化合物(6)是首次从天然界获得的。
YAN Ya-ping , LIU Shi-hai , WANG Zhe-zhi~
2004, 24(10).
Abstract:The acquisition of high-quality RNA is a foundation of launching molecule biological study of Salvia miltorrhiza Bunge. But the isolation of RNA from various tissues of Salvia miltorrhiza is notoriously difficult due to the abundance of soluble polyph
LI Xiao-hong , SHAO Jian-wen , ZHANG Xiao-ping~ , Employment of Biological Resources of Anhui , Wuhu , Anhui , China)
2004, 24(10).
Abstract:In the present study,the karyotypes of Lysimachia qimenensis X.P.Zhang,X.H.Guo et J.W.Shao and L.christinae Hance were observed and compared,which are respectively 2n=2x=24=6m+6sm+6st+6t and 2n=2x=24=2m+4sm+6st+12t.Based on the karyotypical data as we
CHEN Ya-peng , CHEN Ya-ning , LI Wei-hong , ZHANG Hong-feng
2004, 24(10).
Abstract:Taking the Populus euphratica distributed in the lower reaches of Tarim River as a study sample, the relationship between proline, abscisic acid content of P.euphratica and different groundwater levels is particularly analysed at Yahepu, Yingsu and Ar
SONG Wan-ping , ZHANG An-Ling , GAO Jin-ming~ , ZHANG Yu-lin , ZHU Hong-xia
2004, 24(10).
Abstract:In the present study,inhibition of three different polar solvent parts against six phytopathogenic fungi from the ethanol extract of the root of Helianthus annuus L. was studied by growth rate method. The result showed that three extracts had some inhib
2004, 24(10).
Abstract:Temperature not only determines the distribution of plant species but also affects the productivity and quality of crops.The mechanisms of cold tolerance in plants involved in many aspects,such as the change of composition of membrane lipids,the accumulat
2004, 24(10).
Abstract:This paper reviews the study of sex chromosomes, genes involved in sex determination and regulation of sex determination in several plants in recent years .The mechanism of sex determination in plants is discussed preliminarily. We find different plants h
2004, 24(10).
Abstract:The plant sulphate transporters may play a key role on sulphate transport and sulphate absorption of plants. Over 40 genes of sulphate transporters have been cloned from Arabidopsis , wheat, barley, rice and potato, etc. Compare the gene sequences of sul
ZHANG Xiao-mei~ , BAI Jian-rong~
2004, 24(10).
Abstract:乌拉尔图小麦(T.urartu)是四倍体小麦和普通小麦A基因组的供体,它在普通小麦的发育和进化中起着关键的作用。本文首先介绍了该物种的发现、形态特征和在分类学上的位置,然后从遗传多样性、它与小麦属内其它种的关系、构建基因组文库和1Ay高分子量麦谷蛋白亚基基因的研究等方面综述了乌拉尔图小麦的遗传学研究进展。为了加快普通小麦遗传学、发育和进化、新品种选育等方面的研究,本文建议今后应重点从挖掘优异基因、构建遗传图谱及分离探针和微卫星引物等方面对乌拉尔图小麦作进一步的研究。
2004, 24(10).
Abstract:Causes of inconsistent results existed in the study of the rules of secondary metabolite synthesis and accumulation in plant are analyzed. The main reason is the ignorance of the impacts on the synthesis and accumulation of secondary metabolites while col







