Abstract:cDNA capturation is an expression-based gene isolation technique that uses genomic DNA to capture cDNAs directly and thus can rapidly identifies expressed sequences from large genomic region.By means of this technique a captured cDNA library of rice immat

Abstract:丹参(Salvia miltiorrhiza Bge．)是一种重要的药用植物。以组织培养2～13w的丹参幼苗为材料，构建丹参cDNA库并进行大规模EST序列分析，所得序列经NCBI的BLAST工具分析，克隆号为rsmsxl-009377的序列与晚期胚胎丰富(Late Embryogenesis Abundant)基因家族Ⅱ中的成员有较高的同源性。根据其5’单向测序的结果设计引物“5'-GTGCGTAGACACATCGGTTC-3'”继续向3’测序，得到一个全长969bp的序列，序列分析发现该序列包含一个长690bp的开放阅读框(ORF)，编码229个氨基酸，与NCBI注册的脱水素家族基因具有较高的同源性，且含有ⅡLEA蛋白的特征序列，表明本基因可能是一种新的脱水素基因，命名为DHN1，并住GENEBANK上进行了注册，序列号为：AY695932。

Abstract:Studies were made on stomatal length,width,density and their correlation of 3rd leaf in 52-7 with BADH cDNA and its donor control plants Zhong 8 with SEM.The results indicated that 52-7 was obviously different from Zhong 8 in stomatal characters and their

Abstract:通过农杆菌介导法将反义磷脂酶DT(Anti-PLDγ)基因转入美洲黑杨G2中以提高其耐盐性。本研究建立了美洲黑杨G2组培再生体系，确立了美洲黑杨G2叶片分化最适宜培养基和生根培养基，进行了卡那霉素(选择性抗生素)敏感性试验，改良了传统的农杆菌介导转化法。经诱导不定芽及生根阶段卡那霉素(选择性抗生素)连续筛选，获得了21株卡那霉素抗性植株，抗性植株经PCR及PCR-Southern杂交检测，有13株均呈阳性，证明Anti-PLDγ基因成功整合到美洲黑杨G2基因组中。耐盐性实验表明，4株转基因植株抗NaCl能力比对照有不同程度提高。

Abstract:The genomic DNAs extracted from yong levels of rapeseed hybrids "Shaanyou No.8" and its parents were analyzed using polymerase chain reaction (PCR) with arbitrary 10-mer primers to generate random amplified polymorphic DNA(RAPD) markers.Among 100 RAPD pri

Abstract:以台湾桤木 Alnusformosana 引种2年生苗木为研究对象,探讨了台湾桤木的光合生理生态特性.结果表明: 1 叶片净光合速率 Pn 具有明显的日变化.中部叶Pn日变化在生长中期表现为双峰曲线型,高峰分别出现在9:00和15:00左右,峰值分别为15.03μmol·m-2·s-1和12.97μmol·m-2·s-1;在生长初期和末期为单峰曲线型.不同部位叶片Pn大小为:中部叶>顶部叶>基部叶. 2 不同部位叶片Pn表现出不同的季节变化特征.中部叶和顶部叶一年有两个高峰,高峰均出现在6月和8月;基部叶只有一个高峰,出现在6月.光合作用具有较广的温度适应范围.自然条件下叶片最大净光合速率为17.67μmol·m-2·s-1;人工条件下为20.2μmol·m-2·s-1,在新的环境条件下表现出较强的光合适应能力. 3 叶片LCP为33.32～67.47μmol·m-2·s-1,LSP为1332～1656μmol·m-2·s-1,具有较低的光补偿点和较高的光饱和点,表现出较强的弱光利用能力和强光利用潜力. 4 台湾桤木CO2补偿点为54.17～74.98μmol·mol-1,CO2饱和点在800μmol·mol-1左右;羧化效率为0.0270～0.0468.表现出较大光合作用潜力和较广的生存适应能力. 5 通径系数分析表明,Cond、Ci、Vpdl和RH是影响叶片光合速率变化最主要的直接作用因子.

Abstract:Most plants show an inverse relationship between the stomatal parameters (density and index) and atmospheric CO_2.The relationship is affected by C_3 or C_4 plants,amphistomatous or hypostomatous,age of plants and leaves.CO_2 concentration is not the sole

Abstract:In this paper,we compared the growth index of the mature embryos of five cultivars of Ginkgo biloba L.in three basic media (B_5,WPM,DCR).ANOVA analysis indicated that the different among media is more significant than that of among cultivars.No significan

Abstract:The callus of Eucommia ulmoides was used to study the effect of inoculum amount,culture time and times of subculture on callus growth and secondary metabolites production.The results revealed that the callus growth of subculture was suitable when inoculum

Abstract:The changes of C,N metabolic products were investigated when sweet pepper potting seedlings were exposed to different floor level of water supply in media (RWC was 90%,75%,60%,45%,30% respectively) and discussed the relationship with development.The main

Abstract:试验以Ca(NO3)2和NaCl按5：1的比例均匀混合设计0、0．1％、0．3％、0．5％、0．7％的盐浓度5个处理，模拟宁夏不同日光温室的土壤含盐量，以番茄为试验材料，研究了不同浓度盐分对番茄养分吸收的影响。结果表明，在同一生育期内，随着盐分浓度的增大，番茄植株对养分吸收明显减少。在不同的生育期内，对照处理对氮磷钾钙镁的吸收在结果初期达到最大值；0．1％盐分处理对氮磷钾的吸收在结果初期达到最大值，而对钙镁的吸收在收获末期达到最大值；在高盐处理(0．3％以上)中，番茄植株对氮磷钾钙镁的吸收在收获末期达到最大值。

Abstract:研究了12个国内外紫化苜蓿品种在渭北旱塬丘陵沟壑区根系的发育能力。结果表明，根系生物量、根系体积、侧根数在不同紫花苜蓿品种间差异显，在土壤中从表层到深层逐渐递减；侧根的发生部化受不同深度土壤含水量的影响。占0～50cm土层根系生物量66．5％的根系集中分布在0～20cm土层中。而侧根发生的主要部位在0～20cm的主根段，40cm以下没有侧根发生。通过聚类分析，可把12个紫花苜蓿品种分为3类，其中WL-323ML、两香、苜蓿王、巨人在渭北呈塬丘陵沟壑区根系发育好，具有较强的根系发育能力。

Abstract:Cytochemical localization of glycoproteins at the cell surface in the leaves of Lycium barbarum L.was observed by electron microscopy using ruthenium red changes in the cell surface in salt stress.It was found that the structure of glycoprotein at the cel

Abstract:通过对雪松根际进行包埋处理,形成干旱土壤环境,研究了干旱胁迫对雪松生理特性的影响.结果表明,5～10月份,土壤干旱胁迫条件下,雪松光合速率平均值较对照降低了55.7%,蒸腾速度降低了46.4%,气孔阻力增大.光合速率和蒸腾速率的最大值出现在6月份,干旱胁迫下分别为18.262μmol·g-1·s-1和9.325mol·g-1·s-1.进入10月份,雪松仍能维持一定水平的光合速率和蒸腾速率.同时,干旱胁迫下,雪松的水分利用效率略有增大,较对照高4.2%,9～10月份,水分利用效率显著增大,10月份较6月份高263%.

Abstract:The diversity,coverage,density of exotic weed populations were surveyed with square intercept method in invasive habitats in Nanjing.There were thirty-six exotic weed species recorded in spring of this region.The frequency of exotic weed emergence in 74 s

Abstract:本研究旨在比较高寒草甸草地不同退化演替阶段下植物地上部氮磷元素含量、吸收速率以及单位面积累计重量.结果表明:植物生长季内 5～9月 ,3个样地植物地上部N、P含量都是在生长初期最高,随植物生长季节的推移以及地上生物量的增加,其含量逐渐稀释直到生长季节结束,全氮含量范围为36.90～14.90g/kg,全磷的含量范围是2.22～1.06g/kg.不同退化演替阶段由于植物组成的不同,其N、P含量有明显的差异,P的含量中度退化样地最高 1.91% ,重度退化样地最低 1.51% ;N的含量则恰恰相反,重度退化样地最高 26.40% ,中度退化样地最低 18.60% ;原生植被样地介于两者之间,其生长季P的平均含量为1.63%,N的平均含量为20.80%.植物N、P的吸收速率和单位面积累计重量都是随退化程度的加剧而降低,即原生植被样地>中度退化样地>重度退化样地,并且植物营养元素吸收速率与地上生物量生长速率呈正相关关系.

Abstract:通过对秦岭华山松林的野外调查，分析研究了该群落的特征和性质。结果表明：秦岭林区华山松群落主要分布于海拔1100～2100m的中山地带；华山松群落内物种相当丰富，共有81科174属346种；落叶阔叶乔木与落叶阔叶灌木在群落中占很大的比例；群落中草质叶型的植物260种，占80．5％，表明群落的落叶性；华山松群落中乔木层、灌木层、草本层的大多数比较常见的属都属于北温带分布，表明群落植物区系组成的温带性质。

Abstract:The flora of Aishan Mountain is one of the typical representatives of Shandong peninsula with some characteristics as follows:it is abundant in species,containing 488 species of 311 genera,belonging to 96 families.It is notable in dominant family phenomen

Abstract:In Flora Reipublicae Popularis Sinicae,Carex sect.Planatae includes two different types of plants.One possesses interrupted spiciform inflorescence and leaf-like bract.The other possesses densely spiciform inflorescence and scale-like or bristle-like brac

Abstract:采用反相高效液相色谱-二极管阵列的检测方法,对不同产地的10批野生和栽培抱茎獐牙菜药材的水溶性成分进行了分析,建立了抱茎獐牙菜药材的指纹图谱.色谱柱为VP-ODSC18柱 5μm,150mm×4.6mm ,流动相为甲醇-0.02%的磷酸水溶液,检测波长254nm.用文中的最佳条件可较全面地反映抱茎獐牙菜的主要成分,为藏药抱茎獐牙菜的质量控制提供了科学依据.

Abstract:利用柱层析、重结晶等分离手段，从菊科飞蓬属植物一年蓬的脂溶性成分中，分离得到5个化合物，经现代波谱手段鉴定它们为2个三萜：齐墩果-12-烯3β，23-丙叉基-28-醇(1)、齐墩果-12-烯3β-醇(2)和3个豆甾醇衍生物：豆甾-5-烯-3β，7a-二醇(3)、豆淄-4-烯3S，6α-二醇(4)、豆甾-7，24-二烯-3β-醇(5)。其中化合物1是苗次自天然界分离得到的化合物，且其^18C NMR数据及质谱数据为首次报道。对化合物1的酸水解反应得到了化合物齐墩果-12-烯-3β，23，28-三醇(1a)，从而使化合物1的结构得到进一步确证。

Abstract:采用HPLC法，对不同加工方法及不同产地山茱萸中马钱素含量变化进行了系统的研究，并以马钱素为参照物，提出较合理的产地加上流程为：采摘→净选→清洗→水煮软化(2倍水量。80C保温5min)→去核→干燥(60C，连续干燥36h)。

Abstract:Field experiments were conducted at the University of Arid Agriculture,Rawalpindi,Pakistan during spring and autumn 2001 to investigate oil and fatty acid composition of some commonly grown sunflower hybrids in two seasons.Five sunflower hybrids were sown

Abstract:It studies the changes of endogenous hormones and polyamines in cytoplasmic male sterile non-heading Chinese cabbage (Brassica campestris L.ssp.chinensis Makino var.communis Tsen et Lee).Results showed that the microspore was prone to being sterile when t

Abstract:This paper analyses the factors of threaten Kolkwitzia amabilis graebn.Through study on its ecological and biological characteristics and the distribution of at present,study on the dangerous factors.The most important factor was the survivorship conditio

Abstract:S-5120 strain and zuelaemycin with strong antagonistic against Psudoperonospora cubensis Berk.et Curt. were tested by using an spore-germination method and an method on detached-leaves.The protective effect and therapeutic effect of S-5120 strain on downy

Abstract:The effects of AM fungi on the late growth of tobacco leaf in pot culture under five phosphate levels were investigated by inoculation two AM fungi (Glomus mosseae and G.caledonium).The results showed that AM fungi and plant could form good symbiosis rela

Abstract:对火棘多糖的提取工艺、分离纯化以及抗氧化活性作了研究.结果表明:火棘多糖的最佳提取工艺为90℃、9h,料液比为1∶10,提取率3.5%;火棘多糖具有明显的体外清除含氧自由基的作用,DPPH·分光光度法测得SC50为1.87mg/mL;通过分级沉淀、DE-52柱层析分离得到PP-A2、PP-A3、PP-A4和PP-B1、PP-B2、PP-B36种级分;经SephadexG-200柱层析鉴定,PP-A2、PP-A3、PP-B2为均一多糖.

Abstract:对葡萄多酚物质提取分离影响因子进行正交试验，以确定各提取工艺路线的最佳条件，并对各种方法进行了比较。在实验室条件下，有机溶剂提取是进行葡萄多酚物质相关研究的适宜方法，其最佳因子组合为C3D2A2B1，即在70C，50％乙醇浓度，5倍体积比提取60min。同时对提取、萃取的次数和提取时材料的干燥程度进行了研究。

Abstract:This paper studied the activities and isoenzymes of peroxidase (POD) in pearleaf crabapple (Malus zumi) leaves damaged by plum spider mite (Tetranychus viennensis Zacher).The results show that the POD activities increased rapidly along with the extension

Abstract:用高效液相色谱法：甲醇：0．1％磷酸(85：15)，流速为1．0mL／min，检测波长为254nm，柱温为室温，按外标法定量，测定青海省道地药材唐古特大黄中芦荟大黄素、大黄酸、大黄素和大黄素甲醚的含量。结果表明，青海省唐古特大黄中蒽醌衍生物的含量较高，明显高于市售药材，为大黄中的上品。

Abstract:分离并测定黄柏中盐酸药根碱、盐酸巴马汀、盐酸小檗碱的含量.方法:Shim-Pack型C18柱,0.5% w/v SDS-Britton-Robinson缓冲液 pH2.5 -乙腈-三乙胺 0.5∶56∶34∶0.5 为流动相,检测波长:345nm;柱温:40℃;流速1mL·min-1.盐酸药根碱回归方程为Y=38315164.0X-1897.7,r=0.9991 n=6 ,线性范围0.05～0.30μg,平均回收率为95.90%,RSD=3.10%;盐酸巴马汀回归方程为Y=1982872.0X-2471.1,r=0.9998 n=6 ,线性范围0.09～0.54μg,平均回收率100.2%,RSD=2.01%;盐酸小檗碱回归方程为Y=9450164.2X-3456.9,r=0.9999 n=6 ,线性范围0.50～3.00μg,平均回收率103.1%,RSD=1.12%;结果表明:在以上条件下,盐酸药根碱、盐酸巴马汀、盐酸小檗碱成分可以完全分离,并测定了黄柏药材含量.本法可用于黄柏药材、饮片、含黄柏中成药的质量控制.

Abstract:Chinese onion (Allium fistulosum L.) was cultured in vitro to investigate the morphological,physiological and biochemical mechanism of vitrification.The results showed that the vitrified leaves were different from the normal leaves.In vitreous shoots,wate

Abstract:This paper majors the method of extraction of petunia red pigment,furthermore the property and stability of the pigment are determined.The results show that in all tested reagent as abstraction,the effect of 0.1 mol/L HCl is best;and in 0.1 mol/L HCl,the

Abstract:对植物与大气间NH3交换的研究进展进行了综述。大量研究表明，植物冠层能够与大气进行NH3交换．交换的大小和方向取决于细胞间隙与大气NH3分压的差异，即NH3补偿点。大部分农田植物的NH3补偿点大致在1～6nmol NH3／mol air范围内；在自然条件下，NH3补偿点可能接近气孔下腔中的NH3分压，也可能接近大气中的NH3浓度；生长在低氮输入生态系统(如沼泽地和森林)中植物的NH3补偿点几乎为零。当外界大气NH3浓度低于补偿点时，生长的植被会释放NH3：否则，植被就会吸收NH3。影响NH3补偿点的因素主要包括叶温、光照强度和空气湿度等环境因素以及质外体pH、光呼吸速率及谷氨酰胺合成酶活性等内部因素。在植物生长前期，与大气间的NH3交换以吸收为主。后期则以释放为主。植物吸收或释放NH3的大小．因研究者、试验材料和试验条件等不同而有很大差异。植物地上部分对NH3的吸收途径主要有2条，一是通过表皮层吸收；一是通过气孔吸收．通过表皮层吸收的NH3占气态NH3吸收的3％，不是主要吸收途径；NH3吸收丰要通过气孔进行，其吸收量和吸收速率取决于气孔导度、温度及光照等。目前对植物NH3释放机理的解释主要是光呼吸氮循环途径和植物衰老时的蛋白质降解途径．但有关光呼吸氮循环途径在植物体NH3挥发中的作用远末搞清。当前国内、外研究重点主要集中在植物与大气间NH3、交换的方向、强度、NH3补偿点及其影响因素，植物与大气间发生NH3交换的生理机制，人气NH3浓度增加对植物代谢和生理特征的影响等方面，而忽视了对植物生态学特征、源库特征、根系分泌物、养分利用效率和不同植物种群间竞争的影响。因此．进一步深入研究植物与大气间NH3交换。不仅可以丰富植物氮素营养理论，而且也具有十分重要的环境和生态学意义。

Abstract:Most of the important agronomic traits of rice are quantitative in nature, which are controlled by polygenes. In conventional quantitative genetics, QTL analysis was only executed as a whole. In recent years, the construction of highly dense molecular lin

Abstract:According to seed storage behavior, seeds can be categorized into orthodox seeds, recalcitrant seeds and intermediate seeds. Recalcitrant seeds have not maturation drying at the end of seed development. When shedding from their mother trees, recalcitrant

Abstract:Pollen germination and pollen tube growth is a developing process which was highly controlled during the interaction of pollen and pistil.It involved in some physical reactivity,such as recognizing between pollen and pistil,the signal transferring in intr

Abstract:植物胚胎发生是指单细胞的受精卵经过一系列受控的细胞分裂和分化，发育为成熟的多细胞种胚的过程，也是一个基因有序的选择性表达调控的过程。主要从胚胎发生的3个时期即原胚期——极性建成、球形胚-心形胚过度期——区域形态建成、器官形成与成熟期——分生组织形成及发育等方面对基因调控的研究进展作一简要综述。