Abstract:采用磷酸铅沉淀的电镜细胞化学方法,对经冷驯化及未经冷驯化的金边卫矛 EuonymusradicansEmorald&Gold. 以及两者在-5℃下处理1d后的叶肉细胞进行了ATPase的超微细胞化学定位的比较观察,并对冷驯化可提高植物抗寒力及ATPase与植物抗寒性关系进行了探讨.标志ATPase活性反应的磷酸铅沉淀物主要分布在叶片栅栏细胞和海绵细胞的质膜和液泡膜上,ATPase在这两类细胞中的分布及活性无明显差异.金边卫矛幼苗经4℃低温驯化7d后,其质膜和液泡膜的ATPase活性高于22℃下生长的幼苗,冷驯化后的金边卫矛幼苗在-5℃处理1d后,其质膜和液泡膜上仍可观察到ATPase的活性反应,但较低温胁迫前有所降低,且超微结构完整,未见任何伤害.而未经冷驯化的金边卫矛幼苗在-5℃处理1d后,其质膜和液泡膜上的ATPase完全失活,同时细胞的超微结构受到严重伤害.实验结果表明,冷驯化可提高金边卫矛幼苗ATPase在低温胁迫下的稳定性,这种活性变化与植物抗寒力的提高呈正相关,同时还发现液泡膜ATPase活性较质膜ATPase变化幅度大,推测液泡膜可能较质膜ATPase对低温更为敏感.

Abstract:利用基因工程技术构建了含有几丁质酶(Chitinase，Chi．)、β-1，3-葡聚糖酶(β-1，3-Glucanase，Glu．)、萝卜抗真菌蛋白(Raphanus sativus-antifingal protein，Rs-AFP2)的三价植物表达载体，并通过农杆菌直接转化法将三价植物表达载体转入农杆菌EHA105，分别为GC R／pCAMBIA1300／EHA105和RC G／pCAMBIAl300／EHA105，并采用PCR、DNA dot blotting技术对其进行了鉴定分析，证明获得了阳性的重组农杆菌工程菌株。

Abstract:通过对蜜枚和JM的不同倍性西瓜品种的幼苗进行4d、10℃的预冷处理,然后在5℃下低温胁迫8d,结果表明:JM4x和M3x的耐低温能力得到提高,超过2x.冷锻炼诱导多倍体西瓜SOD、POD活性大幅度提高,降低四倍体MDA的含量,增强四倍体西瓜的耐低温胁迫能力.

Abstract:盐胁迫下,DK961(耐盐)和LM15(盐敏感)小麦种子的发芽率(Gr)、发芽指数(Gi)和活力指数(Vi)均显著下降,LM15下降的幅度大于DK961.外源100 mg/L GA3、1×10-7 mol/L ABA和0.1% Ca(NO3)2处理均能缓解盐对小麦种子萌发的抑制作用,对盐胁迫下LM15种子萌发的缓解作用显著好于DK961,并且不同程度地缓解盐处理引起的种子内源GA 1+3含量和α淀粉酶的活性下降,从而降低盐胁迫对种子萌发的抑制作用.表明盐抑制小麦种子萌发的主要原因是盐胁迫导致种子内源GA 1+3含量和α淀粉酶的活性下降.

Abstract:In pot experiments,6-benzylaminopurine (BA) and abscisic acid (ABA) were brushed onto the ears,upper or lower blades of Echinochloa frumentacea to analyze the regulation of partial changes caused in the balance of endogenous hormone on Na~+,K~+ and free p

Abstract:A new wheat rbcL gene cDNA was isolated from a cDNA library constructed by mix mRNA from 4 powdery mildew resistant wheat lines.At present,there are 3 different wheat rbcL cDNA sequences reported.Blast analysis showed that there are only 1 and 3 nucleotid

Abstract:In order to determine the optimum seed pre-sowing treatments and patterns of germination,a greenhouse experiment was conducted to study the effects of abrasion,sulphuric acid,boiling water,cold stratification and seed exudate treatments on the germination

Abstract:以4个马铃薯栽培品种为试材,进行了叶片离体再生研究,结果表明:东农303、鄂1号叶片愈伤组织诱导的最佳培养基为MS+6-BA2.5mg·L-1+NAA0.2mg·L-1;费乌瑞它为MS+6-BA2.0mg·L-1+NAA0.1mg·L-1;夏波帝为MS+6-BA2.0mg·L-1+NAA0.2mg·L-1,愈伤组织的诱导率均可达100%.诱导不定芽分化的最佳培养基分别是:费乌瑞它、鄂1号为MS+6-BA2.5mg·L-1+GA35.0mg·L-1;东农303为MS+6-BA1.0mg·L-1+IAA0.1mg·L-1+GA32.5mg·L-1;夏波帝为MS+6-BA2.5mg·L-1+IAA0.5mg·L-1+GA32.5mg·L-1,其不定芽分化率分别达94.3%、100%、100%和90%.

Abstract:Effects of de-scaled and exogenous hormones treatments on dormancy release and changes of endogenous auxin (indole-3-acetic acid),gibberellic acid,zeatin and abscisic were investigated in sweet cherry trees with High Performance Liquid Chromatography (HPL

Abstract:通过对澳洲青苹茎尖培养及植株再生体系中无菌培养物的建立、初代培养、继代培养、生根培养和移栽五个技术环节的研究，建立了澳洲青苹组织培养快速繁殖技术体系。结果表明：(1)BA0．3～0．6mg／L NAA0．1～0．3mg／L的MS培养基能促进澳洲青苹茎尖生长或大量侧芽的分化，不定芽生长健壮；(2)无根幼苗在含有IBA0．6～1．0mg／L NAA0．1mg／L的1／2MS培养基上暗培养一周后，再进行自然光培养，30d生根率可达到78%以上；(3)生根苗经50mg／L NAA浸渍1～2h后，移入蛭石：腐殖土：田园土=2：1：1的移栽基质中，保温保湿，成活率达80％以上；(4)进一步建立了自然光下生根、练苗一次完成的同步化体系，缩短了繁育时间，简化了步骤，降低了成本。此技术体系为澳洲青苹优良种苗快速繁殖提供了一条新途径。

Abstract:Leaf stomatal conductance,CO_2 absorption and H_2O transpiration of maize ( Zea mays L.) "Shaandan 9" were studied in potted experiment between control (no aquasorb applying),aquasorb rootzone-divided use,and aquasorb roorzone use fully.The results indica

Abstract:Two winter wheat cultivars with large-spike type Yumai 66 and small-spike type Yumai 49 were used to study their activities of enzymes associated with starch synthesis in kernel during grain filling stage.The main results indicated that starch accumulated

Abstract:对农业废弃物无害化处理后,添加一定的无机肥后制作成有机无机复混肥,使其肥料化资源利用.为了进一步研究有机无机复混肥的肥效,2001～2003年在夏玉米上进行了连续3年田间试验,试验结果表明,有机无机复混肥可以促进玉米生长,增加玉米生物产量1958.0～2770.5kg·hm-2,改善玉米产量结构,提高玉米籽粒产量866.0～1144.5kg·hm-2.并能降低玉米耗水系数,提高玉米水分利用效率2.680～3.399kg/ mm·hm2 .在旱地施用有机无机复混肥,可以提高旱地水资源的利用效率.

Abstract:采用HPLC法对陕西省冬油菜区5个试区种植的双低油菜籽粒中硫苷总量和组分含量进行了分析,结果表明: 1 陕西省不同地区的生态条件对双低油菜总硫苷含量有显著的影响,相差达13.14μmol/g,CV为13.30%,5个试区硫苷含量的排序为宝鸡>安康>咸阳>大荔>汉中,地区间达显著或极显著差异;品种间硫苷含量的稳定性有明显的差异,以P01-10品种稳定性最好; 2 在测定的8个硫苷组分中,变幅最大的5个组分依次为2-羟基-3-丁烯基硫苷、4-羟基-3吲哚甲基硫苷、4-戊烯基硫苷、3-丁烯基硫苷和2羟基-4-戊烯基硫苷,极差分别为:5.70、2.69、1.70、1.31和1.03μmol/g,变异系数分别为:17.59%、16.41%、58.84%、10.21%和50.32%,其中以3-丁烯基硫苷稳定性最好,4-戊烯基硫苷稳定性最差; 3 籽粒中硫苷与含油量、千粒重及容重均有显著的相关性,相关系数分别为-0.783、-0.948和0.656,通径和偏相关分析表明,仅硫苷与千粒重偏相关显著为-0.859,含油量和容重是通过千粒重来影响硫苷的.

Abstract:Three cultivars differing in gluten strength (Jinan 17,strong;Yannong 15,middle;Lumai 22,weak) were grown in two environment (soil compacted artificially and the control) to investigate the effects of soil compaction on the grain yield and processing qual

Abstract:用比较样地法调查了青藏高原东缘高寒草甸不同生境 阴坡、滩地、阳坡 植物群落的α及β多样性特征.结果表明?1各种α多样性指数均显示高寒草甸不同生境植物群落多样性顺序为?阴坡>滩地>阳坡.2各种α多样性指数之间均呈正相关,高寒草甸植物群落多样性更多地受均匀度的影响.3在高寒草甸,用物种盖度和地上生物量作为测定指标计算的群落α多样性所得的结果优于用株数作为测定指标计算的结果.4当生境由阴坡向滩地、由滩地向阳坡变化时,β多样性表现出由低到高,再由高到低的趋势,这表明在土壤湿度和日照时间适中时环境异质性大,物种替代速率比较快,β多样性较高.

Abstract:In recent years,due to the runoff which influx to the lower reaches plain of Heihe River persisting subduction and as a result of the regional table of groundwater decline,consequently product a series of problems about eco-environment.By research the rel

Abstract:The geographic distribution and floristic characteristic of Magnoliaceous 7 genera and 23 species in Jiangxi are studied in this paper.First,the distribution-patterns of genera are divided:Tropical Asia,Eastern Asia-North America and Endemic in China,and

Abstract:报道了<秦岭植物志>遗漏的26种(含3个种下分类群)植物,26种植物分属于17属,13科.其中大血藤科(Sargentodoxaceae)和铁青树科(Olacaceae)为遗漏科,大血藤科的大血藤属(Sargentodoxa)、铁青树科的青皮木属(Schoepfia)、五味子科的南五味子属(Kadsura)、樟科的黄肉楠属(Actinodaphne)和虎耳草科的钻地风属(Schizophragma)为遗漏属.

Abstract:This is the second paper that deals with addenda on seed plants of Mt.Qinling. Fagus longipetiolata Seem.of Fagaceae, Morus chinlingensis C.L.Min of Moraceae, Elatostema stewardii Merr.and E.monandrum (D.Don) Hara of Urticaceae, Polygonum paleaceum Wall.e

Abstract:The chromatographic fingerprint of Halenia ellipitica D.Don has been set up by high performance liquid chromatography-diode-arvay detection method for ten batches wild herbs collected in different districts of Qinghai-Tibet Plateau.The result showed that

Abstract:The insecticidal activities of crude extracts of root bark of 13 species of Celastraceae were evaluated by the laboratory bioassay against the 3rd instar larvae of armyworm ( Mythimna separate Walker).The results showed only 2 species exhibited the contac

Abstract:Pleurospermum wrightianum Boissieu.was extracted by supercritical carbon dioxide (SFE-CO_2) and steam distillation (SD).Forty-nine and forty-one compounds were identified after the different two extractions were analyzed by GC/MS respectively.The result s

Abstract:The content of the soluble solids in fruit juice is one of the most important quality index of seabuckthorn fruit juice.This paper deals with the content and it's changing law of the soluble solids in juice of some types of seabuckthorn fruits during diff

Abstract:采用小偃6号ID单体材料作为受体，中优6号、安农91168和皖38为5 10亚基的供体进行杂交，F1代田间苗期取根尖进行细胞学检测，孕穗期镜检花粉母细胞选择单体植株，并对其套袋自交。F2代籽粒在室内进行SDS-PAGE电泳筛选14 15和5 10亚基聚合且纯合籽粒，将5 10优质亚基转入当地主要优质源之一的小偃6号，同时将其聚合效率与正常二体杂交F2籽粒分离结果相比较。分析认为单体材料在亚基聚合中，其亚基分离完全符合一对基因的独立遗传，具有较高的选育效率。

Abstract:Contents of phytic acid,protein,pentosan in the grain of different wheat varieties were tested and analyzed.The result shows:(1) degrees of genetic determination of phytic acid content are low.(2) There is a significant correlation between phytic acid con

Abstract:The chromosome numbers and karyotypes of six Astragalus species from China are reported in this paper for the first time.The results show that all 6 species are diploid.Their chromosome numbers and karyotypes are formulated as follows: A.tanguticus ,2n=16

Abstract:The respiration pattern of bitter gourd should belong to the late peak type.Ethylene production was very little at the primary stage after harvest and at the 4th day the obvious increment was detected and this increment tendency went on until breakdown of

Abstract:Annual apple seedlings of Malus micromalus Makin and M.hupehensis (Pamp.) Rehd. were used to study the changes of UWL intensity,contents of ATP and protein,respiration rate,production rate of active oxygen and some other physiological characters under wat

Abstract:The content of phillyrin in Green Fructus forsythiae,Ripe Fructus forsythiae and leaves of forsythia ( Forsythia suspensa ) from different regions was assyed by HPLC method.The results showed that the content of phyllirin in leaves of forsythia was as 40

Abstract:Observation on ultrastructure of mesophyll cells of 4 species alpine plants grown on Daban Mountain (el.3 900 m) in the northeast of Qinghai-Tibet plateau under TEM.First discover that mitochondria are "phaged" by chloroplast in Taraxacum mongolicum and A

Abstract:With the traditional paraffin section method,the initiation and development were systematically studied.Our observation reveals that no pre-formed adventitious roots exist in the stems of Pogostemon cablin ,all adventitious roots,formed in the cutting,bel

Abstract:活性氧(ROS)的产生是需氧代谢不可避免的结果。在植物细胞中，线粒体电子传递链(ETC)的复合物Ⅰ和Ⅱ是ROS产生的主要的部位。交替氧化酶和可能的内源鱼藤酮不敏感的NADH脱氢酶通过保持ETc的相对氧化状态限制线粒体产生ROS。线粒体基质中的抗氧化酶系统与小分子量的抗氧化剂一道起ROS的解毒作用。ROS除了引起细胞的伤害外，在植物中还能够作为一种普遍存在的信号分子起作用。在低浓度时，ROS能诱导防御基因的表达和引起适应反应；在高浓度时，引起细胞死亡。一氧化氮是植物合成和释放的一种气体，也可作为信号分子调节植物的生长和发育。

Abstract:The Chinese bittersweet, Celastrus angulatus Max.,is a famous insecticidal plant in China.Since 1980's,the insecticidal plant has been investigated from chemistry to biology by poly-subject crossed in our country.This paper reviews the advances and constr

Abstract:In mature cereal seeds,total protein,most of which is storage protein,accounts for about 7%~16% of dry weight.The storage protein has important influence on nutritional quality for humans and livestock and on functional properties in food processing.The c