Abstract:

Abstract:Snowdrop lectin (Galanthus nivalis agglutinin, GNA) gene was introduced into embryogenic calli of elite maize inbred lines Qi319 and Ye515 via Agrobacterium tumefaciens AGL_0, and transgenic plantlets were obtained from resistant maize calli. The resistan

Abstract:对农杆菌介导法转化棉花技术体系进行了综合改进，突破基因型的限制，使多品种或基因型(10个以上)的我国主栽棉花品种(系)转化成功，其中中棉所24号发展成为快速模式化转化品种(转化周期5～7个月，转化率8．1％)，CCRI27、CCRI36等多个品种转化体系基本成熟。不同转化体系的愈伤组织诱导率为10％～40％，愈伤组织胚性愈伤诱导率达到10％～40％，转化率总体平均达到1．8％，并对大批量再生苗的鉴定予以程序化。实现棉花转基因规模化，达到年产转基因棉花植株2000～4000株以上的水平。

Abstract:In order to achieve a pre-harvest sprouting resistance wheat (Triticum aestivum L.), Anti-TrxS gene was transformed into 13 wheat cultivar receptors by means of pollen tube pathway and ovary injection. From 2 036 flowers transformed by pollen tube pathway

Abstract:1BL/1RS male sterile wheat ms(Ae.bicornis)-5-1 and non-1BL/1RS male sterile wheat ms(Ae.bicornis)-V9125 with Aegilops bicornis cytoplasm was studied by microsporogenesis cytological observation. Meiosis showed that two CMS were deferent, the PMC of ms(Ae.

Abstract:The HMW-GS compositions of the most commonly grown wheat cultivars during different times in central Shaanxi plain were studied by SDS-PAGE method. HMW-GS compositions of some germplasms and new lines were also done. The nine types of HMW-GS and their com

Abstract:In this experiment the composition of high-molecular-weight glutenin subunits (HMW-GS) in Taigu-male-sterile wheat recurrent selection population after mutually-crossing two times was analyzed using SDS-PAGE method .The result indicated: the frequencies o

Abstract:Five wheat cultivars with different gluten planted in different sites in Henan were used for investigating the difference of pasting properties of starch by Rapid Visco Analyser (RVA).The results indicated that the starch pasting properties of soft wheat

Abstract:The FtsZ protein plays a key role in bacterial cell division. It forms a ring structure at the site of division to control the process of division. The deficiency or over production of FtsZ can block the normal cell division and lead to a filamentation of

Abstract:以5～7d龄的 丽春 番茄无菌苗子叶作为外植体,研究了子叶外植体对抗生素卡那霉素的敏感性,抗生素卡那霉素对番茄筛选的适宜浓度为70mg/L.通过根癌农杆菌 Agrobacteriumtumefaciens 介导,成功地进行了马槟榔甜蛋白基因MBLII对番茄的遗传转化,获得转化番茄抗性植株,组织化学法有阳性表现、PCR特异扩增及Southern杂交检测出现特异条带,表明MBLII基因已顺利整合到转基因番茄植株的基因组.

Abstract:在人工气候室水培条件下，选用3个不同抗旱类型的冬小麦品种，研究了水分胁迫对冬小麦幼苗叶绿素荧光参数的影响。结果表明：水分胁迫下，冬小麦幼苗可变荧光(Fv)、最大荧光(Fm)、可变荧光与最大荧光比(Fv／Fm)、可变荧光与初始荧光比(Fv／Fo)、光化学淬灭系数(qF)均降低；而初始荧光(Fo)与非光化学淬灭系数(qNP)则升高，说明光系统Ⅱ(PSⅡ)受到了伤害，使得PSⅡ原初光能转换效率(Fv／Fm)、PSⅡ潜在活性(Fv／Fo)降低；光合电子传递、光合原初反应过程受到抑制，起光保护作用的热耗散提高。但水分胁迫下品种间各参数变化幅度不同。除qNF外，其余各参数均为抗旱性越强降低幅度越小，而qNF则升高幅度越大。说明水分胁迫对冬小麦幼苗叶绿素荧光参数的影响与其抗旱性密切相关。

Abstract:在CO2浓度倍增(700 μmol·mol-1)条件下,以5个不同剂量的UV-B辐射对大棚番茄的光合作用及SOD、POD、CAT酶活性的影响进行了研究.结果表明CO2倍增能够明显提高番茄叶绿素含量、净光合速率和抗氧化酶活性.在CO2倍增条件下,低剂量UV-B辐射(<1.163 kJ·m-2·d-1)可以刺激番茄叶片叶绿素含量升高,抗氧化酶活性升高,与CO2的正效应有叠加现象,但对光合作用的影响不大;高剂量的UV-B(>1.163 kJ·m-2·d-1)辐射使植株的叶绿素含量、净光合速率和抗氧化酶活性降低,对植物产生胁迫作用,CO2倍增与UV-B辐射复合处理可以减弱和部分抵消这种抑制作用.

Abstract:研究了蛋白核小球藻在增强UV—B辐射下NO信号的产生。结果表明：NO释放量与UV—B的强度相关。在藻类细胞培养液中加入NOS的竞争性抑制剂硝基精氨酸(LNNA)，不能抑制NO的释放。加入NO清除剂乙酰半胱氨酸(NAC)，能明显减少NO的释放。在无氮和有氮培养基中NO同样的释放。这些结果说明NO生成途径不经过依赖L-精氨酸的NOS和依赖NO3^-和硝酸还原酶，NO的底物不是L-精氨酸和NO3^-在无氮和有氮培养基中同样有NO3^-的形成，NAC减少NO2^-的释放，说明生成了NO2^-有可能是NO释放后生成的。

Abstract:模拟兰州地区夏季臭氧层减薄12%和20%时增强的UV-B辐射2.54kJ·m-2·d-1 T1 和4.25kJ·m-2·d-1 T2 ,以自然辐射为对照,研究2种辐射对番茄幼苗生长、叶绿素和可溶性蛋白质含量以及过氧化物酶 POD 、吲哚乙酸氧化酶 IAA氧化酶 活性的影响.结果表明:低强度、短时间的辐射 T1,10d 对番茄幼苗生长没有影响,而高强度、长时间的辐射可减少生物量累积,降低株高、壮苗指数、叶绿素a、b和总叶绿素的含量,对叶绿素a/b无显著影响;与对照相比,可溶性蛋白质含量在辐射处理10d和20d时均减低,40d时升高;T2处理使POD活性一直显著高于对照;IAA氧化酶活性在辐射10d和20d时增加,40d时显著低于对照.说明在UV-B辐射下光合下降、IAA分解抑制了番茄幼苗的生长.

Abstract:以4～5叶的 二苠 茄幼苗为试材,研究了其在低温弱光 10℃/5℃昼/夜,光强60、120μmol·m-2·s-1 胁迫7d并恢复7d后的光合特性变化.结果表明,低温弱光胁迫后茄子幼苗的净光合速率、气孔导度和叶绿素含量显著降低;光补偿点、光饱和点、光饱和时的Pn、表观量子产额降低;CO2补偿点升高,CO2饱和点、CO2饱和时的Pn、光合能力、CO2羧化效率降低;以低温下较强光照时 120μmol·m-2·s-1 的变化幅度较大;恢复7d后各项指标仍然不能恢复到对照水平.试验条件已对茄子幼苗叶片光合机构的结构和活性造成了不可恢复的伤害.

Abstract:Thermal dissipation method was applied to study the stem sap flow of Betula platyphylla for two consecutive growing seasons. Compared to the healthy Betula platyphylla in the year 2000, diurnal stem sap flow pattern changed significantly after effecting t

Abstract:以氯丙嗪(CPZ)和LaCl3对水稻幼苗根际预处理,阻断其Ca2+·CaM信使系统传导后,研究了干旱胁迫下稻苗膜脂过氧化和保护酶活性的变化.结果表明干旱胁迫下,LaCl3和CPZ根际预处理显著加剧稻苗膜脂过氧化,加剧过氧化氢酶(CAT)活性和抗坏血酸过氧化物酶(APX)活性下降,对胁迫前期超氧化物岐化酶(SOD)和过氧化物酶(POD)活性无显著影响,且CPZ和LaCl3预处理加剧CAT和APX活性下降与加剧稻苗MDA含量积累分别呈极显著和显著正相关.这些结果暗示Ca2+·CaM信使系统可能主要通过调节或影响CAT和APX活性而调节稻苗的抗旱性.

Abstract:This paper put forward the technique of culture by bagging fruit vegetable firstly, and systematically studied the effects of different bag type and the best bagging period on different fruit vegetable, and measured and analyzed the contents of the nutrit

Abstract:研究了太白红杉的平均生殖年龄、最高生殖年龄和最小生殖年龄以及与环境之间的关系。结果表明太白红杉的平均生殖年龄为78．59a，最小生殖年龄为26a，最大生殖年龄为208a；其中南坡太白山最小生殖年龄26a、最高生殖年龄153a、平均生殖年龄72．97a；北坡最小生殖年龄29a、最高生殖年龄208a、平均生殖年龄92a。海拔和年降水与平均生殖年龄呈正相关，坡度与最高生殖年龄呈正相关，最高生殖年龄与最小生殖年龄呈正相关，表明提早生殖会减少太白红杉的生殖寿命。

Abstract:The allelopathy of 6 families, 36 genus, 48 species plant samples in the seeds germination of Sorghum vulgare Pers., Cucumis sativus L., Triticum aestivum L. and Brassica campestris L. were determined. The results showed that the inhibiting rates of 43 ki

Abstract:武陵山地区含有中国种子植物特有属72个，占该区种子植物总属数的7．16％，占全国同类属数的28．69％，占华中同类属数的77．42％。其基本特征为：(1)古老与孑遗的木本植物众多，并存在一定相对年轻的成分；(2)地理成分多样，具明显的亚热带性质，表现出四方汇集和东、西、南、北的过渡性质；(3)水平分布上呈现南、北两个分布中心，垂直分布上以1000m以下的中、低海拔为主；(4)武陵山地区植物区系与神农架、秦岭、湘黔桂交界地区的关系比较密切，而与南岭、武夷山、滇黔桂地区的关系比较疏远。根据武陵山地区中国种子植物特有属的数量、与鄂西～川东特有现象中心(或者三峡中心)所含属的一致性以及地理间的密切联系，可以认为该区是鄂西～川东中心(或者三峡中心)的重要组成部分甚至是核心地带，在华中植物区系中发挥着举足轻重的作用。

Abstract:Two new caffeic esters, nonadecyl caffeate and pentadecyl caffeate (isolated as a mixture) have been isolated together with other known compounds from the whole plant of Ceratoides latens. Their structures were elucidated by means of spectral data and che

Abstract:1993～1999年对西北麦区一种病原菌未知的根病依据柯赫氏证病律进行了病原学研究.结果由来自陕西、甘肃和宁夏等省区麦田的病根标样发现了23个瓶梗霉 Phialophora 分离菌株.经诱导不能产生子囊壳.在PDA培养基上菌落为灰色至浅褐色,气生菌丝致密,灰色.产孢细胞为瓶梗状,长10～20μm,宽3～4μm,无色至浅褐色,顶端有领环颈口.分生孢子无色,有两种,一种长卵圆形,单胞,稍弯曲,6～17μm×1.5～6μm;另一种新月形,单胞,4～8μm×1～1.5μm.附着枝浅褐色,筒形,端部呈钩状,1～2细胞,10～14μm×5～8μm.分离菌株接种小麦,引起根部皮层组织变褐,茎基叶鞘变褐,基叶褪绿,严重发病植株矮化,基叶枯死.该菌与已有瓶梗霉不同,暂定为瓶梗霉小麦致病类型.

Abstract:The activities of SOD, ASP and SOD isoenzymes have been studied in cowpea (Vigna sinensis)seedling leaves damaged by carmine spider mite (Tetranychus cinnabarinus). The results are as follows:(1) 2~8 d after treatment, the SOD activity increases remarkabl

Abstract:This paper study the distribution of NO~-_3-N in the rhizosphere soil of maize under controlled root divided normal irrigation,controlled root divided alternate irrigation and fixed irrigation.The results showed that the distribution of NO~-_3-N in the rh

Abstract:Effect of osmotic stress on K content of flue-cured tobacco was studied with solution culture experiment. The results showed that:In grand growth period, low external osmotic potential caused by PEG-6000 solution stimulated K accumulation in leaves, stem

Abstract:在日光温室滴灌条件下,采用四因素二次回归通用旋转组合设计,对影响甜瓜的灌水上限、钾、磷、氮的耦合效应进行了研究.得到了总产量 y 与灌水上限 x1 、施钾量 x2 、施磷量 x3 、施氮量 x4 的水肥耦合回归模型.分析结果表明:1在本试验条件下水、钾、氮、磷各因素影响甜瓜产量的顺序为K>P>H2O>N;2各因素间存在交互作用,但交互作用未达显著水平.通过计算机模拟,得出了结果期各因素的最佳组合.

Abstract:Peony variety LuoYangHong was used to study the effects of application of ethephon and GA_3 in the fall on bud sprouting and flowering of peony. The results show that application of ethephon promoted leaf abscission, delayed bud sprouting and flowering of

Abstract:利用正交试验设计,从Mg2+、dNTP、引物、甘油4种因素3个水平,对不结球白菜ISSR反应体系进行优化,确立了适合不结球白菜的ISSR反应体系:在20μL反应体系中,含1×PCRbuffer,1.5mmol·L-1MgCl2,200μmol·L-1dNTP,0.5μmol·L-1引物,1%甘油,30ng模板DNA,1UTaqDNA聚合酶.通过梯度PCR测验,确定了适宜的退火温度.

Abstract:Maize seeds are soaked in the solution of extract of Peganum multisectum Maxim Bobr with different concentration, meanwhile the seed germination and seedling growth of maize was studied. The results showed that the germination of seeds and the growth of e

Abstract:The contents of essential oil in Corydalis straminea Maxim were measured and analysed by GC-MS.The result shows that the main contents are long-chain alkylations.Another contents in it are alcohol,ester and ketone and so on.

Abstract:家山黧豆作为人类的食物及动物的饲料，最早可追溯到新石器时代。长期以来，因其营养丰富，具有耐旱、耐寒等优良生产性状，广受世界各地的青睐。特别是在大旱年份，在其它粮食作物绝收的情况下，仍有较好的收成，因此，被视为干旱及半干旱地区首选的优良作物。但由于其含有毒素β-ODAP，使山黧豆的种植受到限制。近年来，由于人类对肉质品的量与质的多元化的需求，家山黧豆这一潜在的、丰产的、高营养的豆科作物，已引起各国学者的广泛关注。从家山黧豆在植物学、遗传学、分子生物学、生态学、营养学、山黧豆中毒、毒理学、毒素ODAP、ODAP的分析方法、ODAP的生物合成途径、养殖业等几个方面的研究进展作了简要综述。

Abstract:Nitric Oxide (NO) is an important active molecule in biology. It is first described as a diffusible multifunctional second messenger in animals since it plays various functions including the relaxation of blood vessels, neurotransmission and defense durin

Abstract:Carotenoids are synthesized from acetyl-CoA via the mevalonate pathway in fungi and plant cytosol/endoplasmic reticulum or from glyceraldehyde-3-phosphate and pyruvate via methylerythritol pathway in bacteria and plastids of plants. The first carotenoid p

Abstract:Environmental factors that impose water-deficit stress, such as drought, salinity and temperature extremes, place major limits on plant productivity. To overcome these limitations and improve plant productivity, marker-assisted selection of quantitative t

Abstract:Wheat is the heterogenous polyploid plant with large chromosomes and high level of repetitive sequences. These characters have limited the progress of wheat genomics studies. Comparative genomics provided a platform for study of wheat genomics by using mo

Abstract:The application and the biological effect of ultrasound in plant physiology research and practice field are reviewed, including the effect of ultrasound on the respiratory intensity, percentage of germination, the situation of rhizogenesis and some certai