Abstract:以植物的胚珠和子房为实验材料,介绍一种用Steedman's wax 包埋对组织切片中的细胞核进行DAPI染色的方法.Steedman's wax 作为一种低熔点多酯蜡,具有与石蜡相似的性质,切片方法同常规石蜡切片,适合于切成厚度大于5 μm的连续切片.Steedman's wax包埋的切片能成功地进行DAPI染色.与用压片法和Technovit 7100或GMA包埋切片法进行的DAPI染色相比,用Steedman's wax 包埋切片法进行的DAPI染色具有廉价、操作简便、可进行连续切片、图象清晰等优点,特别在植物细胞程序化死亡(PCD)的研究中及细胞核DNA含量测定方面,有着较大的应用价值和潜能.

Abstract:The primary vascular system in the seedling of Ligusticum jeholense by utilizing some experimental protocols such as tissue cleaning,paraffin sectioning et al.was investigated.The results included that along the seedling's axial organ,the firstly formed p

Abstract:Casparian strips were isolated from primary roots and needles by enzymatic isolation,the cellular structure was observed under the fluorescence microscope and chemical characterization was analyzed by Fourier transform infrared (FTIR) spectroscopy.The res

Abstract:针对翅果油树愈伤组织诱导的褐变问题，从外植体、碳源、抗氧剂的类型及浓度等的筛选进行了研究。结果表明；幼龄的茎段是诱导愈伤组织的最佳材料。葡萄糖为碳源的培养基能有效地防止褐变。抗氧化剂Vc和Na2S2O3能减轻褐变程度，其中0．15g／L的Vc效果最佳，其次为0．1g／L和0．2g／L。Na2S2O3对褐变的抑制作用不及Vc，3种浓度无明显区别。吸附剂AC不能防止外植体褐变，且抑制愈伤组织的诱导。

Abstract:利用体视显微镜、扫描电镜和光学显微镜，对珍稀濒危植物翅果油树(Elaeagnus mollis Diels)的茎、叶、果、休眠芽等多种器官表面覆盖的表皮毛，进行了详细观察．发现其表皮毛的形态和结构具有多样性，各器官有差异，据此将它们分为分支状毛、星状毛、盾状-星状毛、盾状毛四类，以及一些特殊的表皮毛，如：分支状毛与星状毛或盾状-星状毛的过渡类型称为类星状毛和类盾状-星状毛．叶片上表面的表皮毛，为分枝状表皮毛，且稀疏散布．叶片下表面的白色表皮毛，也多为分枝状表皮毛，但分布较密，叶主脉处最为稠密多为星状毛．叶柄的表皮毛形态呈现一定的梯度变化，远轴端(靠近叶片端)为类星状毛，近轴端(靠近茎的一端)为盾状-星状表皮毛，中间段为星状毛介于前二者之间．茎表面灰色的或褐色的表皮毛，以盾状表皮毛为主，夹杂少量盾状-星状表皮毛，因茎的老嫩其表皮毛形态略有不同．外果皮沟槽内的表皮毛多为盾状表皮毛，嵴部则密集星状或分支状表皮毛．休眠芽的鳞片多覆盖盾状表皮毛，只在鳞片的尖端有少量星状毛和分枝状毛．翅果油树多种器官表面多种表皮毛的存在是其适应高山(海拔800～1300m)环境的一种形态特征．表皮毛具有反射阳光、阻止水分过度散失、保温、防止机械损伤等功能．为探究翅果油树耐早、耐寒、耐高温机理以及组织培养过程中外植体易污染等问题，提供了形态学依据，也为进一步进行表皮毛的发育和分子生物学研究打下了一定的基础。

Abstract:以库拉索芦荟、木立芦荟和皂质芦荟为材料，用扫描电镜观察其叶表皮气孔和角质膜的结构，用高效液相色谱法(HPLC)测定了3种芦荟属植物叶中芦荟素的含量。扫描电镜观察结果表明，3种芦荟叶表皮都覆盖有厚的角质膜，气孔下陷，表现出典型的旱生植物特征。但角质膜的纹饰和厚度在不同芦荟间有显着差异。木立芦荟角质膜表面呈瘤状突起，角质膜厚度为5～6μm，库拉索芦荟和皂质芦荟的角质膜表面较平，库拉索芦荟的角质膜厚度为3～4μm，皂质芦荟的角质膜厚度为8～10μm。高效液相色谱法(HPLC)测定结果表明，木立芦荟叶含芦荟素最高，库拉索芦荟叶含量较低，而皂质芦荟叶含量最低。此外，本文还初步探讨了芦荟属植物叶表皮结构与芦荟素含量的关系。

Abstract:Two anti-insect genes,scorpion toxin (BmKITS) and chitinase,were ligated into two plant expression vectors,pWM101 and pBI101.These recombinant plasmids pBI101-BmKITS-chi and pWM101-chi-BmKITS were confirmed by restriction enzyme and PCR analysis and the r

Abstract:Using patch-clamp techniques,we examined the K~+ channel activity in the plasma membrane of root cells from Arabidopsis thaliana (ecotype Columbia) after NaCl treatment.Inward currents in the root cells were significantly decreased within 5 min after 50 m

Abstract:采用He-Ne激光 5mW·mm-2 辐照方法,对增强UV-B 10.08kJ·m-2·d-1 辐射小麦细胞膜损伤进行修复的研究.结果表明:经He-Ne激光和UV-B复合处理后,小麦细胞膜表面电荷的电泳速度高于UV-B处理组,小麦的MDA含量比单独UV-B辐射后的低,SOD酶的活性增强.说明He-Ne激光和增强UV-B辐射复合处理后可使小麦的氧化酶活性增强,从而使MDA的浓度降低,小麦细胞膜损伤得到了一定程度的修复.

Abstract:The plant bivalent expression vector pBG121 containing PC_(SAG12)-ipt gene was transformed into cotton cultivar CCRI 10 by pollen-tube method.Transformed T_1 seedlings were screened by kanamycin selection,PCR analysis and GUS histochemical staining.12 Tra

Abstract:Erythrocyte membrane Na~+-K~+-ATPase from healthy human blood is prepared by the method of Hypo-Osmosis.The effect of tomatine on the activity of Na~+-K~+-ATPase is studied.The results showed that at lower concentration of tomatine (below 1 mmol/L),it doe

Abstract:发根农杆菌Ar1334工程菌株与商陆叶片共培养,诱导效率不同程度地受到发根农杆菌生理状态、浓度、浸泡时间、共培养时间的影响:采用稀释5倍的处于对数生长期的Ar1334工程菌株,感染商陆无菌苗幼嫩叶圆片5min,在MS培养基上共培养2d,可达最大转化效率82.2%;诱导的根在附加20mg·L-1Kan的MS无激素MS培养基上,呈现旺盛的生长态势和典型的发状根结构特点,经PCR检测扩增出了560bp的rolC基因的目的片断,组织化学检测也证实了GUS基因在发状根中的表达.

Abstract:在野外实地观测锦带花(Weigela florida(Bunge)A．DC．)的开花动态和访花者，在显微镜下观测柱头和花粉的形态及花粉-胚珠比，用TTC法测定锦带花花粉的活力，用联苯胺-过氧化氢法测定柱头可授性。结果表明，锦带花的花粉活力在开花时达70％以上，且能维持3d，之后明显下降。锦带花的柱头在开花3h后多数呈白色、二裂、具可授性，柱头的可授期持续2～3d。柱头与花药之间有5．3士0．9mm的空间距离，自然条件下难于自花授粉．开花当天7．00～10．00之间访花者最丰富，开花第2天访花者减少，开花第3天时访花者更少。在锦带花上记录到的访花者隶属于膜翅目、鳞翅目和双翅目，其中，熊蜂和蜜蜂是主要传粉者。

Abstract:选取山西历山山地草甸中的22个优势种，运用χ^2检验、3个关联指数以及Pearson相关和Spearman秩相关系数进行种间关联和种间相关分析．优势种对间大多呈正关联和正相关，但呈显着和极显着的种对不是很多，各种间相互依存，群落稳定发展。相关分析表明优势种对间大多呈正相关，Spearman秩相关要优于Pearson相关。Pearson相关星座图将22个优势种分为4个生态种组，各生态种组内的种有相同的资源利用方式和生态要求，各种组间有互相转化和演替的趋势。

Abstract:In the paper,the edge effect of deciduous broad-leaved forest in the warm-temperate zone were studied by means of using Shannon-Wiener species diversity index (D),Simpson index (C),the strength of the edge effect index,and importance value (IV) of every s

Abstract:Two-dimensional pattern analysis of community is important in understanding community structure,yet the analysis methods are limited.The two-dimensional sampling using two transects meet at right angles was applied to get quadrat data in this study.The de

Abstract:The classification and ordination of Hippophae rhamnoides subsp.sinensis community in the north Shanxi were studied using two-way indicator species analysis (TWINSPAN) and detrended correspondence analysis(DCA).Thirty-there samples collected from the nort

Abstract:采用χ^2检验方法和相关分析等方法对山西天龙山木本植物群落进行了种间关系分析．结果表明木本植物群落的种间关系以正相关为主，但是负关联的比重也较大，而且还有一定的独立格局存在，表明物种间的关系不大稳定，群落正处于发展演替之中，同时也表明物种对环境资源的竞争并不激烈，物种资源比较丰富。

Abstract:According to the data of field plots,in this paper the author deal with the preliminary characteristics such as the floristic composition,the geographical elements,the physiognomy characteristics and the vertical structure of Forsythia suspensa community

Abstract:采用典范对应分析 CCA 对全国73个自然保护区的种子植物多样性的分析表明:在纬度低于25°N的自然保护区中,物种多样性主要受热量因子和水分因子的影响,并具有较高的种子植物科数和属数值;纬度范围介于25°N和30°N之间的自然保护区,其物种多样性主要受海拔的影响,并且种子植物种数较多,同时有较高的种子植物种属比;在更高纬度范围内,地理及地形因子对物种多样性影响显著,并且随着纬度的增高,纬度因子的作用有增强趋势.

Abstract:There are 281 species of bryophyta,10 varieties and 2 forms belonging to 128 genera and 53 families,among which there are 37 species of liverworts,2 varieties and 1 form belonging to 22 genera and 19 families,244 species,8 varieties and 1 form of true mos

Abstract:The difference of ecological dominance of meadow population on different tourism disturbance was studied by important value and frequency.The result shows that the ecological dominance of different population reflects their response to tourism disturbance

Abstract:Both the receptors and sources of risk that related to the compound biome's ecological risk must account their obviously spatial beterogeneity.As a result of comparative outstanding social economic factor,it shows even more complicated and variety.Shanxi

Abstract:The plankton composition and distribution were investigated in Miyun reservoir from April to October in 2002.122 species of phytoplankton and 36 species of zooplankton were observed in the reservoir.Phytoplankton was predominated numerically by Bacillario

Abstract:报道山西省的淡水红藻植物，共计有15种，隶属于6目，7科，9属，即紫球藻Porphyridium purpureum (Bory) Drew et Ross，暗紫红毛菜Bangia atropurpurea (Roth) Agardh，细弱弯枝藻Compsopogon tenellus Ling et Xie，弯枝藻C．coeruleus (Balbis ex C．Agardh)Momagne，灌木状拟弯枝藻Copsopogonopsis fruticosa (Jao) Seto，异孢奥杜藻Audouinella heterospora Xie et Ling，硬枝奥杜藻A．chalybea (Roth) Bory，矮小奥杜藻A．pygmaea (Ktitzing) Weber-van Bosse，棘刺红索藻Thorea hispida (Thore) Desvaux，鸭形串珠藻Batrachospermum anatinum Sirodot，胶串珠藻B．gelatinosum (Linnaeus) De Candolle，弧形串珠藻B．arcuatum Kylin，绞扭串珠藻B．intortum Jao，细连珠藻Sirodotia tenuissima (Collins) Skuja ex Flint和胭脂藻Hildenbrandia rivularis (Leibmann)Agardh．

Abstract:The comparative study on the DHPDH (Diurnal High Photosynthesis Duration Hours) of different wheat genotypes was carried out for the first time as a important photosynthesis parameter.DHPDH and diurnal photosynthetic capacity (DPC) of flag leaves in diffe

Abstract:Five species crop seeds were cultured separably by extracting liquor of five species bryophytes.The crop seeds were Triticum aestivum,Zea mays,Glycine max,Arachis hypogaea and Phaseolus vulgaris.The bryophytes were Porella platiphylla,Herpetipheuron tocco

Abstract:In this paper,the tissue culture and rapid propagation of Caragana microphylla Lam. segments were studied.The results showed that MS+6-BA 0.5mg/L+IAA 0.01 mg/L is the best fitting optimum medium for inducing bud,1/2 MS+IAA 0.5 mg/L best fitting for rootin

Abstract:以西藏红景天嫩叶和幼茎为材料，研究西藏红景天不同外植体的离体培养技术。结果表明：西藏红景天嫩叶是诱导愈伤组织和芽的理想外植体，诱导松散型愈伤组织有两种培养基，分别是黑暗培养下MS 6-BA2．0 mg／L NAA0．2mg／L和MS 6-BA3．0mg／L NAA0．3mg／L；生芽培养基为MS 6-BA2．0mg／L IAA0．25mg／L；生根培养基为MS IAA0/5～1．0mg／L。

Abstract:Mitochondria or chloroplast polypeptides at differential stages (leaf,bolt,anther) of tuber mustard (Brassica juncea Coss.var.tumida Tsen et Lee) cytoplasmic male sterile line (CMS) and maintainer line (MF) were analyzed on sodium dodycyl sulphate polyacr

Abstract:Wild Arabidopsis thaliana and two salt-tolerance mutants were used for RAPD analysis.Three out of 150 radom primers could detect DNA polymorphism between the two mutants and the result showed that they were a series of near isogenic lines because of their

Abstract:Euonymus chloranthoides Yang is one of the state second class protected species and endemic to China.It distributes in Jinyun Mountain,Beibei and in East Hot Spring Park,Tongjing,Chongqing,China.E.chloranthoides is as a companion species under arborous la

Abstract:通过对大螺旋藻的6种营养成分进行分析表明，大螺旋藻含有61％～66％蛋白质，4．5％～4．7％核酸，6％～8％碳水化合物，4％～5％粗纤维，1％～2％粗脂肪和13～14mg／100g维生素C，这些成分与极大螺旋藻及钝顶螺旋藻的成分很相似。因此大螺旋藻与钝顶螺旋藻、极大螺旋藻同样可被开发利用。

Abstract:Chemical constituents of the stems and leaves of Sambucus williamsii were pretested.It is found that the stems and leaves of Sambucus williamsii contained alkaloids,coumarins,terpenes,triterpenoid saponins,flavonoids,anthraquinones,tannins,etc.The rat kil

Abstract:The ubiquitin/proteasome pathway is regarded as a major system of proteolysis in eukaryotic cells involving in a variety of basic pathway during cell life and death.The present paper reviewed the members of the ubiquitin/proteasome pathway and their bioch

Abstract:Phospholipase D (PLD) is an important phospholipid hydrolase.It is widespread in various plants,animals and microorganism (bacteria and epiphyte).The recent study shows that it has many important functions such as plant cellular signal transduction,membra

Abstract:Molecular mechanism and present improvements of higher plant cytoplasmic male sterility were expounded in mitochondria DNA,chloroplast DNA and plasmid-like DNA.The relationship between nuclear DNA and cytoplasmic DNA was discussed in this paper.

Abstract:DNA molecular markers that were developed by molecular biology is a kind of important genetic markers in recent years.DNA molecular markers have been widely applied to various aspects of research in fruit tree.In this paper,the principles of several main

Abstract:In this paper,the bryophyte's application in medicine,ecological and environment protection,horticulture and agriculture were analysed,the actuality of the bryophyte resource of China was discussed,and at present the protection countermeasures was provide

Abstract:Arnebia euchroma (Royle.) Johnst. is a plant with wide uses.Shikonin derivatives,extracted from Arnebia euchroma (Royle.) Johnst.,were extensively applied in medical treatment,food and other fields.The amount of natural Arnebia euchroma (Royle.) Johnst. h