Abstract:通过对西伯利亚百合不同外植体离体培养、生根培养以及结鳞茎的研究,建立了组织培养快速繁殖技术体系。结果表明:诱导鳞茎不定芽分化的最佳培养基为M S+1.0 BA+0.5 NAA,大于2 cm叶片长的分化率达到135.67%;诱导叶柄不定芽分化的最适培养基为M S+0.5 BA+1.0 NAA,分化率达到28%;诱导叶片不定芽分化的最适培养基为M S+0.5 BA+1.0 NAA+0.1 KT,分化率达到12.50%。最适增殖培养基为M S+0.2 NAA,60 d后增值率达到318%;最佳结鳞茎和生根培养基为M S+9%蔗糖,蔗糖浓度为3%~9%时,对结鳞茎和生根具有促进作用,11%时,对其具有抑制作用。

Abstract:SAP2, an APETALA2 homologous gene, was first isolated from the flower buds of strawberry by homologous cloning. Its full length was 1 820 bp and encoded 435 amino acids. Its sequencing indicated that it had the typical domains of AP2 family. The RT-PCR an

Abstract:A plant expression vector driven by CaMV35s promoter and carrying tomato chitinase gene was constructed and transformed into a strain(Agrobacterium tumefaciens),EHA105,by Freezing & Thawing.The expression vector was transformed into the plants of Zhongyu

Abstract:对Flame　Seedless葡萄胚珠、胚乳及胚的发育和败育过程进行了系统的细胞学观察，并以有核葡萄品种北醇为对照对Flame　Seedless的胚珠重量及纵横径进行了测定。结果表明：（1）Flame　Seedless内、外珠被及珠孔发育异常，20％～30％的子房未出现受精现象，受精的胚珠重量和纵横径随着胚珠的发育，先呈增加趋势，在某一时期达到最高值，随后下降,最终退化为痕迹。（2）大约有30％的胚乳核能够进行正常分裂，形成胚乳组织，胚乳在花后30d开始退化,其余胚乳核分裂异常。合子在盛花后第19天进行第一次分裂，经过二细胞原胚→多细胞原胚→球形胍，此后胚开始退化；也有的合子始终不发生分裂，最终退化。（3）由于胚乳分裂异常和提早退化，胚缺乏营养致使其发育中止和败育。（4）Flame　Seedless　胚挽救的最佳接种时期在花后第37天。

Abstract:用不同浓度配比的生长素和细胞分裂素诱导梨枣叶片和茎段愈伤组织的产生,并研究了不定芽诱导的最佳配方,建立了梨枣叶片和茎段的再生体系.结果表明,梨枣叶片愈伤组织诱导的最佳培养基为MS+2,4-D 1.5 mg·L-1+6-BA 0.5 mg·L-1;茎段为MS+2,4-D 1.0 mg·L-1+6-BA 0.5 mg·L-1.叶片不定芽诱导的最佳培养基为MS+IBA 0.1 mg·L-1+6-BA 1.5 mg·L-1. AgNO3能阻止叶片外植体褐化并有效地促进叶片愈伤组织分化.茎段能在同一培养基上产生愈伤组织并直接分化出不定芽.

Abstract:运用辣椒细胞质雄性不育系21A和保持系21B为试材，比较分析了过氧化物酶（POD）同工酶在幼叶和不同发育时期花蕾中的表达特征。结果表明，辣椒花蕾中的POD同工酶谱带均比叶片中的更丰富，表现出明显的组织特异性。不育系21A和保持系21B间的酶谱在幼叶和小花蕾（纵径0．8～1．5mm）中基本无差异，但从中花蕾（纵径2～3mm）开始，保持系21B的一些酶带（POD4b和POD4c）染色开始减弱。在大花蕾（纵径4．5～5mm）和特大花蕾（纵径6．8～7mm）时期，不育系比保持系分别多2条酶带（POD2c和POD4b）和1条酶带（POD2c），表明辣椒细胞质雄性不育系21A与保持系21B花蕾中的POD同工酶表达与小孢子的发育过程相关，其表达差异发生在细胞学上观察到的败育之前。

Abstract:The Stone cell formation and distribution in the fruits of Kuerler fragrant pear were anatomically examined, which showed that the fruits of Kuerler fragrant pear began to form stone cells fifteen days after full bloom and the diameter and density of the

Abstract:PEG6000 was employed to study the physiological responses of the seedlings of four Alnus formosana provenances and the nursery stocks of two Alnus cremastogyme provenances to water stress and the result of the study revealed that: (1) As the water stress

Abstract:Two varieties with large-size spikes and big kernels,914391 and Lumai 22(L22),and two varieties with medium-size spikes and medium-size kernels,Jinan17(J17) and Lumai 18(L18),were chosen to study the differences between the photosynthetic capabilities of

Abstract:以土垫旱耕人为土为供试土壤进行盆栽试验,在不同施氮水平条件下,以NR9405、9430、偃师9号、小偃6号陕229、西农2208、矮丰3号等7个冬小麦品种为材料，研究不同品种冬小麦对氮肥反应敏感性。结果表明：7个品种中，9430、西农2208、偃师9号和NR9405属于氮肥较敏感品种，小偃6号和矮丰3号属于氮肥敏感性较差品种，陕229居中。小偃6号对氮肥的反应敏感性最差，属于典型的对氮肥反应不敏感型品种。根据对不同品种干物质转移量、干物质转移效率和转移干物质对籽粒贡献率的分析，将供试品种分为两类：一类是在生长后期（开花至成熟）主要靠同化产物的继续大量形成和转移而影响籽粒产量，可称为“后期同化累积型”；另一类在生长后期则主要靠开花前干物质的转移而影响产量，可称为“后期物质转移型”。偃师9号、西农2208和9430属于比较典型的“后期同化累积型”，而小偃6号属于比较典型的“后期物质转移型”。将不同品种地上部分干物质、氮素累积对氮肥反应的敏感性对比分析后发现，“后期同化累积型”品种对氮肥反应相对敏感，“后期物质转移型”对氮肥反应的敏感性差,这种差异性可能与“后期同化累积型”在生长后期仍需继续从介质中吸收大量氮素，以满足同化需要，而“后期物质转移型”在生育后期主要靠花前吸收氮素转移和再分配以满足花后同化需要，从介质中吸收的氮素有限有关。

Abstract:The antibiotic and physiological effects of thiram thiophanate-methyl sulphur,Carbendazin and low temperature storage on the cut flowers and their flowering rates and vase shelf lives of 'Yingsuzi',a cultivar of Chinese peony, were studied.The results rev

Abstract:采用营养液水培,以抗低氧能力不同的2个黄瓜品种为试材,研究了Ca2+对黄瓜幼苗植株生长和根系无氧呼吸酶活性的影响.结果表明,在低氧胁迫下,LDH、PDC和ADH活性提高程度与幼苗的抗低氧性和Ca2+浓度密切相关.与抗低氧性较弱的"中农8号"相比,抗低氧性强的"绿霸春4号"幼苗根系LDH活性增加缓慢,而PDC和ADH活性增加幅度较大,因此增强了植株对低氧胁迫的抗性.低氧胁迫下,营养液中8 mmol·L-1 Ca2+处理能显著提高根系ADH活性,降低LDH和PDC活性,0 mmol·L-1 Ca2+处理表现出相反的规律.由此可以说明,低氧胁迫下,Ca2+能够提高ADH活性,降低LDH和PDC活性,可增强黄瓜植株对低氧胁迫的抗性.

Abstract:采用盆栽土培法,对31个甘蓝品种苗期生长对铜胁迫的耐性及铜吸收和积累的差异进行了研究。结果表明,400 m g.kg-1Cu2+处理下,不同基因型甘蓝的耐性及吸收、积累铜的能力具有显著差异。地上部干重的抑制率变幅为-25.0%~95.7%,铜对地上部的抑制作用大于根系。根系Cu2+含量变幅为212~893 m g.k-g 1,地上部变幅为32~87 m g.kg-1,根系Cu2+含量显著高于地上部;根系Cu2+积累量变幅为5~78μg/盒,地上部变幅为8~116μg/盒。地上部Cu2+含量与地上部抑制率呈极显著的正相关。根据聚类结果,31个甘蓝品种分成3类,平均地上部干重抑制率分别为76.0%、40.3%和-2.1%,表明其分别属于铜敏感型、中间型和耐性型。

Abstract:The effects of long term intensified UV-B radiation on the photosynthetic rates and pigments,and antioxidative systems of three typical plants, Kobresia humilis, Elymus nutans and Potentilla saundersiana, in alpine meadows were studied in the field. The r

Abstract:The overlapped zones of forests are highly rich in biological diversity.Shannon-Weiner's diversity index,Simpson's dominance index,Margalef's richness index and Pielou's evenness index were used to study the dynamic diversities of the community species in

Abstract:Taking the rehabilitation of the mangrove forest in Qi'ao Island lying near the outlet of Pearl River as an example and resorting to the growth models for large-size wetland plants,the paper quantitatively analyzes the competitive relation between Sonnera

Abstract:Indoor and field experiments were conducted in Minqin Psammophyte Garden to investigate the phenophases, germination temperatures and growths of Ceratoide. lanata, which were introduced from the desert region and grassland region of the United States, as

Abstract:连续2年地不同退化程度天然干旱荒漠草原围封后群落的土壤种子库进行研究。结果显示，土壤种子库物种数、种子密度和植物生活型在年际间、封育与放牧样地间及退化梯度问均有明显差异。种子库物种数为封育高于放牧样地；退化梯度相比，物种数除2003年封育样地随退化程度加剧呈减少趋势外，放牧样地及2004年各样地均随退化程度加剧而增加。种子岸密度结果显示．2003年种子库密度明显高于2004年；各封育样地2年中土壤种子库密度显著或极显著（P＜0．01）高于放牧样地；退化梯度相比种子库密度除2003年封育样地随退化程度加剧而急剧增加外，其余各样地种均随退化程度加剧而明显下降。植被生活型分析结果为，2003年以一年生草本植物种出现频率最高，各样地分别达94．0％～98．4％．多年生草本及灌木出现频率则很低，分别为1．59％～5．15％和0．03％～0．4％，2004年则为多年小草本植物种比例上升．且显示出封育高于放牧、退化程度愈轻增幅愈大的趋势。2年的结果显示种子库物种Shannon－Wiener多样性指数和Peilow均匀性系数均以封育低于放牧样地。种子库密度与地上植被盖度相关性分析表．2年结果中仅一年生草本和2003年灌木的土壤种子库密度与相应地上植被盖度之间具相关趋势和显著（P〈0．01）相关。

Abstract:Two meadow plants, Elymus nutans and Potentilla anserina of Kobresia humilis meadow were studied al different grazing intensity in consecutive five years. The results indicated that E. nutans appeared the highest in its planl height, tiller numbers, above

Abstract:73 soil samples of algae taken from biologically crusted soil of 9 quadrats located in the Gurbantonggut Desert were used to culture and identify their algae on purpose of analyzing the composition and ecological distribution of the algae in the Gurbanton

Abstract:研究了垂穗披碱草(E lym us nutans)在不同光照强度、不同土壤养分和不同密度下的生长和繁殖特性。结果表明增加施肥量使垂穗披碱草的新增分蘖百分数、分蘖大小、营养繁殖和有性繁殖输出及其效力明显增加,贮藏生长效力相对减少;提高光照强度增加新增分蘖百分数、营养繁殖输出、有性繁殖输出和效力以及贮藏生长效力,减少营养繁殖效力;低密度下新增分蘖百分数、分蘖大小和营养繁殖效力显著增加,高密度下营养繁殖输出、有性繁殖输出和效力以及贮藏生长效力较高。在高施肥×高光照、高施肥×高密度和高光照×高密度处理下,植物有性繁殖输出和营养繁殖输出显著高于其它处理。低密度条件下新增分蘖百分数较高,高密度条件下有性繁殖效力较高。三因子互作对性状无显著作用。营养繁殖效力与有性繁殖效力无显著相关,这两者与贮藏生长效力为负相关。以上结果说明丰富的资源(高施肥、高光照)有利于植物生长和繁殖,种内竞争水平对体内的资源分配会产生很大影响。这些特性既与环境因子有关,又与植物性状间的内在联系有关,共同决定该种群在群落中的动态和稳定性。

Abstract:The subalpine meadows in Luyashan Mountain are one of the typical meadows in north China and play an important role in water and soil conservation, eco-tourism resource exploitation and bio-diversity protection and so on. The meadows in west Luyashan Moun

Abstract:A bioactive compound(A)was isolated from Chimonanthus praecox seeds by silica gel column chromatography and antibiotic activity tracking,and identified as d-calycanthine by such techniques as mass spectrometry and nuclear magnetic resonance spectrum techn

Abstract:选择7月份的华山松球果。用GC-MS分析了健康果和受害果萜类挥发物成分。结果表明，华山松球果挥发成分25种，其中单萜17种。倍半萜8种，7种主要成分均为单萜组分，它们是a-蒎烯、β-非兰烯／4-侧柏烯、β-蒎烯、β-香叶烯、D-柠檬烯和异松油烯。球果受害后，单萜、倍半萜种类没有变化，除β-蒎烯含量减少外，单萜总量和各组分的含量明显增加。其中β-非兰烯／4-侧柏烯含量增加最多；而倍半萜各组分相对含量变化很小。同时，比较了水蒸气蒸馏和二氯甲烷浸提的分析结果。认为水蒸气蒸馏法不适合华山松球果挥发成分的研究。

Abstract:Weight method and Gas chromatography-Mass spectrometry were adopted to determine the fat and fatty acid contents of the seeds of three wild plants, S. glauca, S. corniculata and S. salsa, which grew on saline-alkaline land of west Jilin Province. The resu

Abstract:对山楂叶总黄酬生长积累动态及提取工艺进行了研究，结果表明：（1）年生长周期中，山楂叶总黄酮含量有两个高峰期．其中第一个生长高峰期（6月15日～8月1日）峰值较高，持续时间较长；（2）各因素对山楂叶总黄酮的提取率影响是不均等的，影响最大的是料液比，其次是温度和时间。最佳提取工艺为：80Ｃ下，用碱度为0．25％的氢氧化钠乙醇溶液（50％乙醇）回流提取2次，每次lh．料液比为l：20。

Abstract:Polygonatum qinghaiense Z. L. Wu et Y. C. Yang,a new species from Qinghai, China, is described and illustrated. The new species is closed to P. hookeri Baker, but differs from it by oblong or angusti-elliptic leaves, yellowish or white flowers, 3-nerved p

Abstract:It is not only economic but also capable of meeting the requirements of RT-PCR to extract the total RNA from the in vitro differentiated shoots of banana by the modified Guanidine Thiocyanate method. A specific band of 739 bp could be amplified form the R

Abstract:Drop method, conical flask fumigation and leaf disc method were adopted to study the contactpoisoning, fumigating and food-refusing bioactivities of Ligusticum sinense Oily. Extracts to Mythimna separata Walker and Plutella xylostella L. as well as prelim

Abstract:The growth and mineral nutrient balance of black locust clones were studied with one-year pot-planted black locust clones,BH_(327) and BH_(56).The results showed that BH_(56) was not remarkably inhibited by salt stress and its tissues,maintained high K~+,

Abstract:以普通小麦小偃6号为材料,研究从SDS-PAGE中快速回收和累积蛋白亚基的方法。采用制备电泳(17 cm×17 cm×1.5 mm)进行分离,KC l快速染色,将目的蛋白条带切下,置于透析袋中电洗脱回收蛋白亚基。结果表明,在蛋白提取过程中加入四乙烯吡啶与常规方法相比能够更好地分离高分子量麦谷蛋白14和15亚基,回收后的14亚基经电泳检测为单点纯。本方法为高效分离回收小麦中高分子量麦谷蛋白提供了新的技术思路。

Abstract:采用蚕豆根尖微核实验和姊妹染色单体交换实验,研究亚硒酸钠(0.01～10.0 mg·L-1)对蚕豆根尖细胞的遗传损伤效应.结果表明一定浓度的亚硒酸钠(Na2SeO3)可导致蚕豆根尖细胞有丝分裂指数下降,间期细胞微核频率明显增高,且Na2SeO3的上述效应具有一定的剂量效应关系;同时Na2SeO3可诱导细胞姊妹染色单体交换(SCE)频率显著增高.研究表明,亚硒酸钠对蚕豆根尖细胞具有遗传毒害作用,并有可能对人体产生遗传损伤.

Abstract:在营养液栽培条件下，以番茄（合作906）为研究材料，设计不同的CO2及养分浓度处理，采用定期取样的方法研究番茄定植后根重齄的动态变化及CO2与不同养分供应强度的交互作用对根中碳氮含量与碳氮比的影响。结果表明：番茄苗期根干物质在生长前期积累速率较慢，中后期积累速率较快，在育苗后期CO2对根干物质积累的影响大于前期，根干重对CO2的响应随营养液离子浓度的改变而变化，表明对天番茄幼苗根的生长发育，CO2施肥结合高养分浓度的营养液，才能达到最佳效果。定量分析番茄根干鲜重与生长时间的关系，结果表明：生长条件的改变，会改变番茄根系的生长，对于根鲜重．在较高的CO2条件下，1／2山崎番茄营养液与1／4山崎番茄营养液里生长的根系鲜重在拟合方程中以幂函数拟合得到的相关系数最大，其余处理以二次曲线方程拟合得到的相关系数最大；对于根干重，在较高的CO2条件下，1／2山崎番茄营养液里生长的根系干重在所拟合的方程中以幂函数拟合的相关系数最大，其余的处理以二次曲线方程拟合的相关系数最大。CO2降低了在1／2山崎番茄营养液中生长的根系中的N含量，升高其它营养液处理中的根的N含量，降低了在1／2、1／4山崎番茄营养液中生长的番茄根系中Ｃ的百分含量，增加在1／8、1／16山崎番茄营养液中生长的番茄根系中Ｃ的百分含量，增加所有营养液浓度条件下的Ｃ、N总量，降低根系中的Ｃ／N比，在同一CO2条件下C／N比随营养液浓度的降低而升高。

Abstract:Capsicum was pot planted and inoculated with seven strains of actinomycetes to study the root-colonizing capabilities and inducing effects of the strains on PAL and PPO activities in the leaves of capsicum twenty-four days after its inoculation by Petri d

Abstract:Mutation is capable of making crops change their one or more specifictraits and thus the mutation-based improvement of plant quality can greatly help fruit production. Conventional mutation-inducing methods are often limited by explant availability and re

Abstract:N-acylethanolamines are the fatty acid amides that are the products of N-aclyated phoshatidylethanolamine hydrolysis and capable of being further hydrolyzed or oxidized with amidase and lipoxygenase in plant tissues. As a trace component of cellular membr

Abstract:Zinc participates in a wide range of physiological and biochemical processes as an important trace element and thus the paper introduces the ZIP(ZRT/IRT-like proteins) and CDF families of zinc transporters in details.ZIP family (ZRT/IRT-like proteins) of