Abstract:佛手是芳香科枸橼类植物,有很好的药用和观赏价值,但其品种较为单一,对其进行遗传改良和品种选育显得十分必要.为了将基因枪转化方法应用于佛手的遗传改良和品种选育,本实验以GUS基因为报告基因,利用基因枪方法将其转入金华佛手外植体内,通过检测GUS基因在佛手叶盘中的瞬时表达情况,分析了外植体的幼嫩程度、射程、氦气压力、真空度、轰击次数等参数对GUS瞬时表达的影响,并研究了最佳检测时间.结果表明,以幼叶为受体,在射程为6 cm、氦气压力为7.584×106Pa,真空度为88.046 kPa条件下,2 d后可检测出GUS基因的最佳表达活性.轰击次数多少对GUS基因瞬时表达影响不明显.

Abstract:Hybrid rapeseed seed purity test system with RAPD technique was established on a simple and rapid method of DNA extraction on rapeseed.2 primers were singled out from 814 primers(10 mer) to distinguish "Qinyou No.7"(CMS hybrid,Brassica napus L.) and its p

Abstract:用RAPD技术进行新疆核桃早实特性的分子标记研究，用180个10-mer随机引物分别扩增早实和晚实近等基因池DNA，筛选出5个多态性引物，结果只有引物OPG15（5’-ACT GGG ACT C-3’）在早实近等基因池及其个体中能重复扩增出一条约710bp的特异片段OPG15 710，而晚实近等基因池及其个体中无此特异片段，将OPG15 710克隆于pUCm-T载体，筛选阳性克隆用限制性内切酶Pst1消化，表明克隆片段大小正确。实步分析认为，OPG15 710可能是与核桃早实特性相关的分子标记，该标记仍在研究之中，该研究将为进一步克隆早实基因，实现早实优质，探索早实机制奠定基础。

Abstract:It is one important approach to clone functional genes.Vprdrf4,a cDNA fragment of the expression gene that differentially displays itself between non-inoculated leaves and the leaves inoculated with powdery mildew,was obtained from Baihe35-1 belonging to

Abstract:With the cDNA in the fruits at the enlarging and maturing stages as the templates and according to the gene sequences of the expansins in other plants,the degenerate primers were constructed to conduct RT-PCR.The product of the amplification were combined

Abstract:本实验旨在建立金钱槭属植物的AFLP反应体系。并应用PstⅠ＋GAA／MseⅠ ＋CAC引物组合对金钱槭属植物进行了选择性扩增，获得了清晰稳定的扩增图谱，表明该反应体系对金钱槭属植物的AFLP分析是可行的。对影响金钱槭属植物基因组DNA的制备、酶切、连接以及扩增反应等因素进行了分析讨论。

Abstract:紫杉醇酰化酶多基因家族是催化红豆杉紫杉醇合成过程中的各酰化酶基因的总和。该基因家族的不同成员之间具有很高的保守性，本实验以该基因家族中的紫杉烷2α-O-苯甲酰转运酶（TBT）基因克隆为例，从引物设计与PCR参数优化角度，总结了针对类似高保守性多基因家族的基因克隆的经验，表明分别在保守性极高部位设计第一次扩增引物及在保守性极低部位设计巢式引物，并采用touchdown PCR程序及高效能的Zaq DNA聚合酶是成功的关键。

Abstract:以东方百合(L ilium orien ta l hybrids)“索邦”作为研究材料,通过高频再生系统的建立和抗生素敏感性实验建立了百合稳定高效试管苗小叶离体基因转化的直接分化受体系统.结果表明:鳞片诱导分化培养基以M S+6-BA 1.0 m g.L-1+NAA 0.1 m g.L-1为宜,试管苗小叶柄分化最佳培养基为M S+6-BA 2.0 m g.L-1+NAA 0.3m g.L-1,试管苗鳞片叶分化最佳培养基为M S+6-BA 1.0 m g.L-1+2,4-D 0.3 m g.L-1;确定了试管苗不同部位适宜的抗生素筛选浓度,卡那霉素筛选浓度小叶柄为80 m g.L-1,鳞片叶为120 m g.L-1,羧苄青霉素筛选浓度为300 m g.L-1.

Abstract:Based on the known HMW-GS 1Bx14 promoter and coding region,the specific primers for 1Bx14 were designed and synthesized.Using the genomic DNA from 8 wheat cultivars,a 400 bp or so fragment was amplified in 4 materials carrying the 1Bx14 subunit,while othe

Abstract:根据苎麻组织自身含有较多的本分类，粘状物及黄酮类等次生代谢物质的特点，对CTAB法进行优化后用于提取苎麻组织总DNA。在研磨过程中加入抗氧化剂PVP，提取缓冲液加入终浓度6%的PVP和4%的β-巯基乙醇，可防止整个提取过程中溶液变褐；在第2次用氯仿/异戊醇抽提时，加入10%的CTAB和4%的NaCl高盐溶液，以除去多糖。经几种改良方案提取总DNA的ISSR-PCR扩增结果验证，以同时加入抗氧化剂和高盐溶液的CTAB-4法效果最好。

Abstract:The development of Bupleurum chinense DC.roots was studied by observing the tips and central parts of one-year-old and two-year-old Bupleurum chinense DC.taproots transversely and longitudinally by paraffin sectioning,and the central structures of the one

Abstract:The uhrastructures ,especially stomatal arrays ,frequencies ,rumules ,large strumaes and cork-cell rumules of leaf epidermises of two wild rice species of Chinese origins and two rice varieties were comparatively studied with the scanning electron microsc

Abstract:In vitro Euphorbia pulcherrima seedlings were domesticated after their transplantation and the anatomical changes of their leaf structures were under study at the same time. The results indicated that they presented a surviwd rate of 98%, on perlite matri

Abstract:Secondary metabolite biosynthesis is regulated by development and inducement on and then this study explored the effects of tissue differentiation and induction on taxol synthesis in Taxus chinensis and resorted to quantitative PCR to analyze the dynamic

Abstract:为了探讨低剂量微波对增强UV-B辐射损伤菘蓝(Isatis indigotica Fort.)的修复作用,将经过10.08 kJ·m-2·d-1辐射损伤(PAR=220 μmol·m-2·s-1)的菘蓝幼苗分别经0、3、6、9和12 s等不同时间的微波辐照(126 mW·cm-2,2 450 MHz),然后测定其幼苗MDA含量、紫外吸收物质含量、抗坏血酸含量以及3种抗氧化酶SOD、CAT和POD活性.结果表明,增强UV-B辐射损伤菘蓝在微波的作用下其菘蓝幼苗中SOD、CAT和POD活性及紫外吸收物质含量、抗坏血酸含量提高,MDA含量得到显著的降低,说明微波对增强UV-B辐射伤害菘蓝幼苗具有修复作用.但是,随着微波剂量的增加,这种修复效应减弱,甚至消失.上述参数的变化说明适量时间的微波处理可以提高菘蓝对增强UV-B辐射的抵抗能力,并在此基础上初步探讨了微波的修复机理.

Abstract:采用L I-COR 6400便携式光合测定系统测定并研究了乌兰布和沙漠白刺叶不同水分状况下光合速率及其叶绿素荧光参数的变化特征.结果表明,随着白刺水势降低其净光合速率和气孔导度快速下降,当水势降低到一定值时,净光合速率、气孔导度几乎不变;而叶绿素荧光指标与叶水势关系在高水势时随着叶水势下降叶绿素荧光指标值近似不变,当水势下降到一定值时近似呈直线下降.白刺水饱和状况下的光补偿点为(43.1±4.8)μm o l.m-2.-s 1,光饱和点为(676±150)μm o l.m-2.-s 1;叶生长初期和叶成熟期净光合速率水势补偿点(净光合速率为0时的水势)分别为-3.65 M Pa和-5.76 M Pa,NPQ初始水分胁迫水势分别为-2.20 M Pa和-6.63 M Pa.研究指出运用净光合速率水势补偿点和非光化学猝灭初始水分胁迫水势可评价白刺对干旱环境的适应性.

Abstract:用不同浓度(0、0.1、0.2、0.4 m o l.L-1)的N aC l处理BG 110培养的发菜细胞,结果显示,发菜光合速率与叶绿素荧光强度随N aC l浓度的升高先增加后降低,当N aC l浓度为0.1 m o l.L-1时光合速率与叶绿素荧光具有最大值,表明发菜细胞培养物能耐受一定浓度的盐胁迫.以BG 110+0.4 m o l.L-1N aC l为对照,在BG 11+0.4m o l.L-1N aC l的胁迫实验中,光合速率与叶绿素荧光强度下降较慢;丙二醛、脯氨酸含量较低;类胡萝卜素含量较高,表明在培养液中添加外源硝酸盐后可以缓解N aC l对发菜细胞培养物的生理胁迫效应,增强其抗盐性.

Abstract:运用开顶式气室(OTC-1)研究了O3胁迫对原位条件下冬小麦叶片的光合色素、脂质过氧化和抗氧化系统的影响.结果表明:随着O3浓度的升高,各个生育期小麦叶片中叶绿素含量下降,叶绿素组成发生改变,且在灌浆期变化明显;叶片相对电导率(REC)增大,丙二醛(M DA)含量增加;过氧化氢酶(CAT)、过氧化物酶(POD)活性和类胡萝卜素(C ar)含量降低,超氧化物歧化酶(SOD)活性在O3浓度低于1×10-7(φO3)时逐渐升高,而后急剧降低.可见O3促进了小麦叶片老化,加剧了膜脂过氧化,破坏了抗氧化系统功能,影响了叶片的正常生理代谢.

Abstract:研究了土壤干早胁迫下小麦（Triticum aestivum L.）旗叶和穗光合作用的差异及其与耐旱性的关系。结果表明，穗光合活性对干旱胁迫的敏感性低于旗叶叶片。干旱胁迫下。穗光合速率下降幅度远小于叶片，类胡萝卜素含世较高，色素含量、PSI活性下降幅度均小于叶片。且PEPC活性诱导增强，暗示穗器官较叶片有较强的耐逆性。

Abstract:在温室中通过严格控水的方法，对4个不同水分处理下，12个黑杨杂交无性系间长期水分利用效率（WUEL）、δ^13C和光合参数（Gs、Tr、Pn、WUEL，Cj、Pmax、LSP、Ce）的差异进行了研究，并分析了无性系间WUEL和δ^13C差异与光合参数差异的相互关系。结果表明：不同水分处理下，δ^13C和Gs、Tr、Pn、WUEL、Cj差异极显著。随水分胁迫的加剧，δ^13C和WUEL增加，而Gs、Tr、Pn、Cj减少。同一水分处理下无性系间WUEL和δ^13C差异显著，Gs、Tr、Pn、WUEj、Cj差异极显著。无性系问WUEL和δ^13C的差异在4个水分处理下表现出一致性．即WUEL最优的是J2，其次为J6、J7、J8、J9，同时这些无性系的δ^13C较高。Gs是影响WUEj的关键因子，Gs低的无性系WUEj高。J2、J6、J7、J8、J9的净光合速率（Ps）和瞬时水分利用效率（WUEj）虽不是最高，但其最大净光合速率（Pmax）大，光饱和点（LSP）高，羧化效率（Ce）大，光合能力强。无性系间δ^13C和WUEL差异的主要影响因子是Gs和光合能力，J2、J6、J7、J8、J9都具有Gs低和光合能力强的特点。各水分处理下δ^13C与WUEL，均呈正相关，但由于水分引起的δ^13C差异远大于无性系间的差异，因此二者的相关性仅在充分供水条件下表现较好．相关系数为0．7077。

Abstract:The sap flux in the trunks of planted Tamarix elongata trees was continuously monitored with Greenspan SF200,a thermal-pulse sap-flow sensor,and the soil water content in Tamarix elongata rhizosphere at the depth of 3 meter was measured with a tube-time d

Abstract:The distributions of wild-fruit forests in Yili River Valley of the Tianshan Mountain were investigated and the relationships of the occurrence and evolutions of the forests with climatic factors in the valley were accordingly analyzed in light of the mul

Abstract:Physiological and biochemical indexes of the leaves of seven species of Caragana,including the contents of free proline,MDA,protecting enzymes(POD and CAT),were measured,and different species introduced from same regions as well as same species inhabiting

Abstract:The leaf characteristics and their relationship of Cotinus coggygria in arid river valley located in the upper reaches of Minjiang River with environments factor in terms of its altitude gradients were studied.The parameters measured in this study were pa

Abstract:There are 122 species,80 genera and 30 families in the lower reaches of Tarim River. They are dominantly composed of dicotyledons and their majority is perennial herbaceous plants. This reveals that in the region,the species succession is the type of yo

Abstract:It is shown by field survey and data analysis that Dashanfeng Mountain has 98 species and 2 variants, 58 genera and 33 families of ferns. Its dominant families are Dryopteridaceae, Polypodiaceae and Athyriaceae, and its dominant genera are Dryopteris, Sel

Abstract:The field investigation and statistical data processing show that there are 809 species, 395 genera and 101 families of wild seed plants in Jingpohu Lake Nature Reserve,of which gymnosperms covers 12 species, 7 genera and 3 families. The reserve consists

Abstract:以GB18406．1-2001无公害蔬菜标准为评价依据．对陕西省8个蔬菜主产区的127个蔬菜样品中汞、砷、铅、镉和铬5种重金属污染状况进行分析评价。结果表明，陕西省主要蔬菜产区蔬菜重金属污染以铅污染为主。茄果类和瓜菜类蔬菜铅污染严重，绿叶类蔬菜铅污染相对较轻．其它4种重金属污染未超过国家标准，其中各产区均未检出镉。铅污染程度的大小顺序足成阳秦都〉宝鸡岐山蔡家坡〉汉中汉台〉渭南临渭。成阳泾阳、宝鸡太白、汉中城固、渭南大荔蔬菜产区5种蓖金瞒均未超标．具备发展无公害蔬菜生产基地条件。

Abstract:The paper reports seven species distributing in Guangdong Province,Pteris splendida Ching ex Ching et S.H.Wu var.longlinensis Ching et S.H.Wu,Pteris oshimensis Hieron.var.paraemeiensis Ching ex Ching et S.H.Wu,Adianthum juxtapositum Ching,Asplenium leiboe

Abstract:本试验对91（260）3-3-8对白粉病的抗性再次进行鉴定，进一步确定该材料对白粉病完全免疫。为了研究其抗白粉病基因的遗传规律，用感病材料陕225、小偃6、邯6172、豫麦49与该材料杂交得到F1、F2后代群体，发现F1抗白粉病；陕225／91（260）3-3-8F2、小偃6／91（260）3-3-8 F2、邯6172／91（260）3-3—8 F2群体抗感比例为3：1，表明“91（260）3—3—8”与感病材料陕225、小偃6、邯6172相比有1对抗白粉病基因的差异；豫麦49／91（260）3-3—8 F2群体抗感比例不完全符合3；1，其原因有待于进一步探明。

Abstract:Four genotpyes of spring wheat were pot planted at different P levels and their acid phosphatase activities,root lengths,root radiuses ,root dry weights ,root/shoot ratios ,shoot dry weights and yields were measured at different stages of wheat growth to

Abstract:The traits and distributions of 34 local jujube varieties of fresh use in north Shaanxi province were investigated and the effects of such meteorological factors as rainfall, sunshine and temperature on early-maturity,medium-maturity and late-maturity juj

Abstract:Young Lippia triphylla stems with buds were employed as the explants to conduct the rapid propagation of Lippia triphylla,which showed that the optimal medium for axillary bud induction was MS+0.2~0.5 mg/L 6-BA+0.1~0.3 mg/L,and the buds of the explants co

Abstract:为了研究大孔吸附树脂纯化山茱萸总皂苷的动态吸附工艺,运用静态吸附与解吸试验对大孔吸附树脂进行筛选,然后通过单因素试验、正交试验和方差分析确定了大孔吸附树脂吸附山茱萸总皂苷的最佳操作条件.结果表明,HPD-300树脂对山茱萸总皂苷的吸附和解吸性能较好.确定的最佳吸附条件为料液浓度3.5 m g.mL-1,上柱速度3.5 BV.h-1,pH值为7.0.HPD-300大孔吸附树脂可较好地纯化山茱萸总皂苷.

Abstract:The paper briefly introduces allele loci and their expression characteristics of rice waxy gene,and expatiates the expression regulation of rice waxy gene in terms of the first nucleotide of waxy gene intron 1 ,waxy gene splicing mode,cis-acting member an

Abstract:The paper deals with research advances of sucrose phosphate synthase(SPS) in terms of its basic properties and its relations with fruit-sugar accumulation,relation of its related enzymes with exogenous stimulations,ethylene relation with respiration and f

Abstract:Diosgenin is an important pharmaceutical raw material. The paper expatiates the effects of the duration and temperature and enzymatic pretreatment of the fermentation and the acid concentration,pressure and duration of the hydrolysis in diosgenin producti

Abstract:Seed dormancy mechanisms mainly focus on seed permeability,inhibitor action and photofrin transformation.Testa inhibition probably results from physical or chemical properties of testa,and can lead to the penetrability changes of water,light,air and solut

Abstract:As the important part of cytoskeleton, microfilament skeleton is composed of actin and actinbinding proteins,and widely exists in eukaryotic cells. In recent years,great numbers of researches show that there exist abundant microfilaments in the pollen and