Abstract:The tracheary elements in secondary xylems of Actaea asiatica root and rhizomes were observered with a scanning electron microscope and found to contain abundant types, which mainly included tracheids, tracheid-like vessels, fibriform vessels,and typical

Abstract:The pollens of 18 plants of Typhonium Schott and its genetically close genera, 8 first reported plants and eleven endemic plants in China were studied with a scanning electron microscope. The results show that the plants of four genera of Tribe Areae have

Abstract:Three interbedded specific primers were designed according to the band (469 bp) of flavonoid 3'-hydroxylase gene amplified from genomic DNA of Nanjinghongxu (Prunus mume) ,and they were combined with a primer bank consisting of six short random degenerate

Abstract:Inbred F1 and crossbred F1 of transgenic paulownia carrying transferred gene shiva-1 were obtained and PCR was adopted to test their progenies. The results indicated that the exogenous gene segregated at the ratio of 3 : 1 in the progenies of the inbred F

Abstract:PCR扩增hrpZ基因片段，克隆到pGEM-T载体中，经EcoRI／Xho1酶切、连接将hrpZ基因插人大肠杆菌表达载体pET32b（＋）硫氧还蛋白下游，构建重组表达质粒pET-hrpZ，再将其转化至E．coli BL21（DE3）中，经筛选得到阳性克隆子，IPTG诱导表达HrpZ蛋白。SDS-PAGE显示，目的蛋白在重组菌株中得到了可溶性高效表达。该重组蛋白分子量为55．8kD，与理论值大小相符。采用叶片穿刺法，融合蛋白具有诱导烟草过敏反应的生物功能。

Abstract:Sixteen crossings with European seedless grape cultivars as the female parents and Shuanyou of Chinese wild Vilis (V. amurensis cv. Shuangyou) ,Beichun from V. vinifera X V. amurensis and 8 European cultivars as the male parents were conducted and then 55

Abstract:The leaf explants of three potato varieties were used as the subject materials to in vitro screen so-matically variant clones of potato by means of crude Phytophthora infestans toxin and the results showed that the leaves and calluses of different varieti

Abstract:利用细胞壁提取法从葡萄采后致病菌Alternaria alternate(Fr．)Keissl中提取出激发子。在采前一周用激发子处理葡萄，研究激发子诱导葡萄采后抗病效果。结果表明：葡萄经激发子处理后，果皮内与抗病有关的过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)活性显著增强，酚类物质和木质素含量也显著提高，贮藏期自然发病率和发病指数降低，其中以125μg·mL-1处理效果最好，果实发病率和发病指数比对照降低500％左右。

Abstract:以对生玉米的雌、雄幼穗为外植体，研究了不同质量浓度激素及其组合对愈伤组织诱导、再分化苗和试管苗生根的影响，建立了对生玉米离体培养再生体系。结果表明：长度为15-17 mm的雌、雄幼穗能够诱导出质量较好的愈伤组织，但只有来自于雄幼穗的愈伤组织才能再生成苗。适合愈伤组织诱导的培养基为Ms+1．5-2．0 mg·L-12，4-D+0．5 mg·L-16-BA+0．5 mg·L-1 NAA+500 mg·L-1脯氨酸+1000 mg·L-1水解酪蛋白+30 g·L-1蔗糖；适合愈伤组织再分化培养基为MS+1．5-2．0 mg·L-1 6-BA+0．1 mg·L-1 NAA+500 mg·L-1水解酪蛋白+30 g·L-1 蔗糖；适合试管苗生根培养基为1／2 MS+0．25-0．5 mg·L-1 IBA+20 g·L-1蔗糖。

Abstract:基因组DNA甲基化对基因表达起着重要的调控作用.本研究采用MSAP方法,对2个玉米杂交种及其相应亲本DNA 5'-CCG G位点胞嘧啶的甲基化水平进行分析,比较了2个玉米杂交种与其相应亲本的甲基化类型与差异.研究发现,2个玉米杂交种Mo17×Suwan 5和Suwan 1×太3221的F1代的甲基化敏感扩增多态性(MASP)比率分别为39.1%和40.1%,均略低于其相应的双亲(39.8%和39.7%、44.6%和43.2%).2个杂交种的全甲基化(双链CmCGG)率分别为24.4%和23.3%,而其相应亲本Mo17、Suwan 5、Suwan 1和太3221分别为17.1%、24.4%、24.6%和21.6%.4个玉米亲本的MASP比率变化范围为39.7%～44.6%,平均为41.8%,全甲基化比率为17.1%～24.6%,平均为21.9%.杂交种与其相应亲本比较有4种类型的变化A型,F1与其亲本甲基化模式相同;B型,去甲基化;C型,超甲基化;D型,次甲基化.杂交种F1代DNA的甲基化模式与其双亲比较,发生了较大的改变与调整.F1代基因组的杂合性与基因组DNA甲基化模式的重新调整有关.

Abstract:The study screened 9 pairs of highly polymorphic primers from 32 pairs of primers for tomato by SSR and analyzed the genetic polymorphisms of 24 tomato germplasms. It totally identified sixty-four bands with the nine pair of primers,and 3-12 bands or 7.1

Abstract:The study employed 34 primers to analyze the RAPD polymorphisms among eleven Prunus avium and Prunus pseudocerasus varieties of European and Chinese origins. All the primers but Sl090 could tell between the varieties of European origin and the varieties o

Abstract:The genetic polymorphisms of the isozymes of 40 Haloxylon ammodendron plants collected in the oasis to desert transitional zone of Fukang of Xinjiang were preliminarily studied by discontinuous SDSPAGE. 24 enzymatic systems of Haloxylon ammodendron were e

Abstract:The HMW-GS compositions of the sampled F_3 kernels and parental kernels of each plant in the F_2 populations formed by crossing five parents were assayed by SDS-PAGE.The relationships of the HMW-GS compositions of the F_3 kernels of each F_2 plants with t

Abstract:The attributes of Robinia pseudoacacia,Pinus tabulaeformis plantations and natural Populus davidiana,Betula platyphylla and Quercus liaotungensis forests in Ziwuling Mountain of the Loess Plateau were comparatively studied by standard quadrat method.The r

Abstract:Population competition is one of the ecological processes affecting ecosystem evolutions.Based on Xu Cai-ling and Tilman's research works,the paper establishes the mathematical model of the competition among metapopulations by integrating competition coef

Abstract:选取中条山中段植物群落的24个优势种,采用2×2列联表的χ2检验、Ochiai关联度指数、Pearson相关和Spearman秩相关分析进行种间关系研究.结果表明:1在所有种对中,呈极显著和显著相关的种对数所占比率较低,大部分种对呈负联结趋势;2χ2检验只有与Ochiai关联度指数结合,结论才比较合理;3Spearman秩相关分析灵敏度较高,优于Pearson相关分析;424个优势种可划分为5个生态种组,同一生态种组内,各种间具有较强的正联结,表明其资源利用方式和生态要求相似,而不同组间联结较为松散.槲栎、槲树、榛子、连翘等呈独立分布.

Abstract:The bryoflora in Tianhuashan Mountain Nature Reserve of Shaanxi were preliminarily studied through field investigations and literature references,and this shows that the reserve has abundant and diverse bryoflora,which number 180 species,95 genera and 46

Abstract:以辣椒CapsicumannuumL.幼苗为材料,研究了辣椒幼苗叶片中活性氧清除系统对低温弱光的响应.结果表明:随着低温弱光胁迫程度和时间的增加,辣椒幼苗叶片中POD活性提高,但SOD和CAT活性下降,MDA含量增加,细胞膜透性增大;就温度而言,临界低温15℃/8℃比偏低温19℃/12℃对植株的影响更为显著;在偏低温19℃/12℃下,弱光90μmol·m-2·s-1使POD活性上升更大,而SOD和CAT活性的下降、MDA含量的增加和细胞膜透性的增大更小,而在临界低温15℃/8℃下则相反;陇椒2号耐低温弱光能力强,各项指标均优于七寸红.

Abstract:One-year-old in vitro apple seedlings were laid under the treatment of half-rhizospere drought resulting from the addition of polyethylene glycol (PEG-6000,20% w/v) to its modified Hoagland's nutrient solution and then compared with those under the treatm

Abstract:The rhizosphere pH value of Pinus yunnanensis Franch. in the P. yunnanensis forests at different ages of the stands located in the red-soil regions of central Yunnan province,and the rhizosphere pH value of P. yunnanensis seedlings treated with different

Abstract:The culture matrixes,leaf osmotic potentials,relative leaf conductivities,GST activities and O2^- producing rates of Puccinellia tenuiflora seedlings were studied at different Na2CO3 concentrations to probe the patterns of GST activity in the chloroplasts

Abstract:The soluble sugar and proline contents,and the MDA ,SOD,POD activities in the leaves of several perennial alpine plants ,and the physiological characteristics of the plants during their growth were studied. The results showed that Kobresia humilis,Elymus

Abstract:以2个优选的甜仁山杏的幼果为试材．以生产中主栽大扁杏品种“优一”和野生山杏（山苦1、山苦2）为对照，通过冷冻处理，以胚褐变率、电导率和MDA值为指标，研究了甜仁山杏优株的抗寒性。结果表明。供试2个优选的甜仁山杏品系胚褐变率低，半致死温度（LT50）较对照低0．51～0．94℃。供试5个品系抗寒性的顺序依次为山甜1〉山甜2〉山苦1、山苦2〉“优一”。

Abstract:以青藏高原药用植物麻花艽为材料，研究了西宁和海北两个地区麻花艽叶片的净光合速率和叶绿素荧光参数的日变化进程。结果表明：在中午太阳辐射较强时两地麻花艽叶片的净光合速率（Pn）均下降，下午随日间光强的减弱逐渐上升，形成双峰曲线；海北麻花艽叶片的净光合速率（Pn）及其日变幅均低于西宁。随日间光强的增加麻花艽叶片的PSⅡ最大光化学效率（Fv／Fm）、PSⅡ的潜在活性（Fv/Fo）下降，非光化学猝灭系数（NPQ）则上升，黄昏各参数都恢复到接近早晨的水平，表明未发生光合机构的破坏；一天中海北麻花艽叶片的Pn、Fv／Fm、Fv／Fo均低于西宁，表明随海拔的升高、光强的增加，海北麻花艽热耗散增多，午间光抑制加重。

Abstract:The photosynthetic characteristics of the leaves of Leontopodium leontopodioides growing at three different altitudes (2 300,2 700 and 3 800 m above sea level) in the northeastern part of Qinghai-Tibet Plateau were comparatively studied.As a result,it was

Abstract:应用柱层析、薄层层析及重结晶等方法，从木贼科问荆属植物节节草（Equisetum ramosissimum）的全草中分离得到17个化合物，经现代波谱技术鉴定了它们的结构，分别为：5α，6α-环氧-β-紫罗兰酮-3-O-β-D-葡萄糖甙（1）、loliolide（2）、环阿尔屯烷～24（30）-烯-3β-醇（3）、环阿尔屯烷-22（23）-烯-3β醇（4）、麦角甾-6，22-二烯-3β，5α，8α-三醇（5）、木栓醇（6）、芹菜素（7）、芫花素（8）、芫花素-5-O-β-D-葡萄糖甙（9）、芹菜素-5-O-β-D-葡萄糖甙（10）、木犀草素（11）、槲皮素-3-O-β-D-葡萄糖甙（12）、山奈酚-3-O-β-D-葡萄糖甙（13）、山奈酚-3-O-β-D-葡萄糖-7-O-β-D-葡萄糖甙（14）、腺嘌呤核苷（15）、β-谷甾醇（16）和β-胡萝卜甙（17）。其中化合物1～5、15为首次从该属植物中分离得到。

Abstract:Fiher-paper diffusion method was adopted in lab to test the anti-microbial activities of nine extracts,four solvent-extracted extracts and five eluates obtained with DIAION HP20 at different ethanol concentrations from Smilax scobinicaulis roots ,to six c

Abstract:In the course of studying the genus Astragalus from China,some new synonyms are reported here for the first time. Of them,A, dulanensis Y. H. Wu is reduced to the synonymy of A. variabilis Bunge;A. heqingensis Liou f. and A. mongutensis Lipsky are reduced

Abstract:用3种不同保鲜液对郁金香品种“摩力”的切花保鲜效果及其花瓣中蛋白质、丙二醛含量及膜相对透性进行了研究，结果表明，不同保鲜液均能维持切花瓶插期间较高的保水能力，增大花径，使花茎挺直、延长花朵每天开放时间和盛开期天数。进一步试验表明，在切花瓶插期间，6-BA和PP333减缓花瓣中蛋白质的降解速度，降低花瓣膜相对透性和丙二醛上升的幅度，从而延长切花瓶插寿命。

Abstract:The volatile oil in the leaves of the Chinese medicinal herb Pistacia chinensis Bunge was extracted by steam distillation. It was assayed by Gas chromatography/mass chromatography to contain 99 chemical compounds,which mainly included many kinds of long-c

Abstract:The leaves,flowers and fruits of Isodon henryi (Hemsl.) Kudo was used to extract their essential oils by steam distillation,respectively.The components of their essential oils were separated and structurally identified by gas chromatography/mass spectrome

Abstract:The active constituents in the stems and leaves of ten Caragana plants collected in Northwest China were systematically pre-tested by round filter paper chromatography and test tube method. The results showed that all the tested samples contained esters ,

Abstract:miRNAs are small-molecule non-encoding RNAs which widely exist in plant genomes and they functed as a group of negative regulators,mainly inhibiting gene expressions on posttranscriptional level by guiding the cleavages of target genes or attenuating the

Abstract:As an effective evolution strategy of angiosperms,double fertilization can enhance the survival of the embryos,seeds and seedlings in which endosperms play a key role in the development of seeds and embryos.However,a small minority of angiosperms that per

Abstract:Medicinal polyploid plants attract great attention because of their gigantic organs,high yields and important medicinal and economic values. The paper mainly deals with the chromosome-doubling method and mutagen types for medicinal plants and other factor

Abstract:It is an important field of plant genetics to explore the variation patterns of quantitative traits for genetically manipulating them. The developments of molecular DNA markers,and QTL mapping and the complete sequencings of Arabidopsis and rice genomes g