Abstract:The conventional paraffin section was adopted to study megasporogenesis and gametophyte microsporogensis as well as male and female gametophyte development of Saposhnikovia divaricata( Turez.)Schischk. The results were as follows :(1)In microsporocyte mei

Abstract:Transmission electron microscopy was adopted to study the origins of vesicular inclusions in the vacuoles of Ammopiptanthus mongolicus,which were composed of vesicles differing in size and shape.They originally occurred in cytoplasm and were later swallow

Abstract:Paraffin sectioning was adopted to study anatomic structures of vegetative organs of Thellungiella halophila,a halophyte.The primary structure of its roots showed a layer of big and highly vacuolated cells and a small number of root hair.Its cortex consis

Abstract:Leaf structure of biennial seedlings of Cyclobalanopsis multinervis and Michelia foveolata were observed by using structural botany method with the treatments of full sunlight and shaded. It is shown that long-term shaded treatment causes the epidermis ce

Abstract:Paraffin sectioning with optical microscopy was adopted to examine the stem and leaf morphological structures of 18 Eupatorium adenophorum Spreng. communities and the results demonstrated that all the communities appeared to differ in stem and leaf morpho

Abstract:孢子发育时期与花蕾形态特征、花药颜色具有相关性。本试验目的是确定黄瓜花药的最佳培养时期，并确定对应的选蕾标准。对基因型、预处理等花药培养主要影响因素研究结果表明：不同基因型小孢子单核中后期花蕾长度不同，形态上没有差异；同一基因型的小孢子发育不同时期的花器特征和诱导率均有差异，单核中后期花药诱导率最高；低温预处理有利于保持小孢子的活力；花药培养中以黑暗中4℃、48～72h低温预处理最有助于愈伤发生。实验结果表明，黄瓜花药培养中小孢子最佳培养时期为单核中后期，取蕾标准为：花蕾长度0．90～1．50cm，绿色，瓣尖未张开，花药白绿色或淡绿色。

Abstract:以秦艽的叶和下胚轴为外植体,成功地诱导出愈伤组织和再生植株.诱导愈伤组织最合适的培养基为附加2 m g·L- 1 2 ,4 - D和0 .5 mg·L- 1 6 - BA的MS培养基,诱导率可达到10 0 % .愈伤组织转移到附加2 mg·L- 12 ,4 - D和0 .5 mg·L- 1 KT和5 0 0 m g·L- 1 L H的MS培养基上进行继代培养,增殖后的愈伤组织转移到附加0 .1mg·L- 1 2 ,4 - D和0 .5 m g·L- 1 6 - BA的MS分化培养基上进行分化,其分化率可达到86 .6 7% ,将分化出的芽转接到不加激素的MS上,结果可生长出大量的分化苗.

Abstract:分析了雄性不育系高粱314A、13A与3种苏丹草杂交组合F1代的生育、株高、穗型、分蘖性、产量、光合性能及细胞遗传学等特性.结果表明各杂交种F1植株高大,平均株高343～369 cm,穗型呈双亲中间型,种子活秆成熟,生育期130～137 d,分蘖性强,单株平均分蘖数6.3～9.0个,花粉可育率91%以上,自然结实率63.88%～67.79%;F1鲜草产量比各自父本苏丹草增加16.17%～32.73%,种子产量增加41.40%～52.97%,杂种超亲优势19.29%～48.68%,茎叶比3.05～3.66;F1净光合速率、气孔导度、胞间CO2浓度均明显超过其各自亲本,CO2固定能力强;F1代PMCMⅠ染色体均能正常配对,平均构型为2n=2x=20(10Ⅱ),但棒状二价体频率明显高于其双亲,原亲本间的遗传组成仍存在着一定的差异;试验证明母本314A、13A的雄不育发生在减数分裂后的小孢子形成期.

Abstract:The root tips of ten common buckwheat cultivars (Fagopyrum esculentum) and seven tartary buckwheat cultivars (F.tataricum) planted in China were examined to study and compare the chromosome numbers and karyotypes of the cultivars, and the results showed t

Abstract:Two barnase-cloning methods currently reported,independent cloning and barstar gene-protected cloning of barnase gene were comparatively examined.When four plasmids were respectively used to independently clone barnase gen,a small number of positive clone

Abstract:采用农杆菌介导法将外源三价抗虫基因Bt-CpTI-GNA导入彩色棉中，经分子检测已整合到受体植株体内，并已得到表达。目前已得到纯化株行。外源标记基因NPTII检测在T。代就有分离现象，各代检测淘汰3～4次才能基本清除分离株或非转化株。抗虫性检测表明转三价抗虫基因彩色棉对棉铃虫具有较高抗性，而对棉蚜则作用小大。

Abstract:Six heterogeneous wheat lines carrying six different types of Aegilops cytoplasm and belong to Chris were used to conduct the direct and reciprocal crosses with nine wheat varieties,which were widely adopted in wheat production,for systematically studying

Abstract:以十字花科植物芸芥 Eruca sativa 、白菜型油菜 Brassica rapa 和甘蓝型油菜 Brassica napus :武39- 1、武4 3- 1、武5 0 - 2为试材,在蔗糖衬垫法分离纯化m t DNA方法优点的基础上,通过4组不同离心力配比,采用差速离心法、改变缓冲液A的成分以及增加缓冲液的用量,建立了一种简便提取十字花科植物mt DNA方法.研究结果表明:分离细胞破碎组织的离心力、分离线粒体的离心力以及纯化线粒体时的离心力分别为10 0 0 g、2 0 0 0 0 g、180 0 0 g时可分离到高产量的线粒体,且在缓冲液A 蔗糖0 .5 m ol·L- 1 ,Tris- Cl5 0 mm ol·L- 1 ,EDTA5 m mol·L- 1 ,BSA 0 .1% ,PVP 0 .5 % ,0 .1%β-巯基乙醇,p H=7.5 中加入0 .5 % PVP可提高mt DNA的产量,经DNA电泳检测、RAPD扩增证明此方法得到的mt DNA可完全达到RAPD标记和其它分子生物学研究的要求.

Abstract:以亚洲百合品种Pollyanna和东方百合品种Sorbonne为试材,在比较异硫氰酸胍法、SDS/酚法与CTAB-L i Cl法提取总RNA效果的基础上,针对百合组织中富含多糖的特点,在Sorbonne提取RNA中加入特殊除多糖步骤,改进了CTAB- L i Cl法.结果表明,改进CTAB- L i Cl法能有效去除多糖,提取到的RNA2 8S r RNA亮度约为18S r RNA的2倍,A2 6 0 / 2 80介于1.8～2 .0之间,A2 6 0 / 2 30为2 .0 ,Pollyanna RNA产率为36 .3μL·g- 1 ,Sor-bonne RNA产率为10 .2 μL·g- 1 ,经RT- PCR获得了特异性条带,说明用改进CTAB- L i Cl法从百合花瓣中提取到的RNA质量好、产率高、完整性强,完全适合于进一步的分子生物学研究.

Abstract:通过对苹果黑星病菌基因组DNA的SSR反应中一些重要参数进行优化,结果表明,最适反应体系为:2 5μL体系中,10×Buffer Mg Cl2 2 0 mm ol/ L 2 .5μL ,d NTP 10 0μmol·L- 1 、引物0 .5μmol·L- 1 、Taq DNA聚合酶1.5 U ,DNA模板2 m g·L- 1 ,dd H2 O 19.5μL .PCR扩增程序为93℃,2 min,5 7℃,30 s,1个循环;72℃,1m in,93℃,30 s,5 7℃,30 s,4 0个循环;72℃,10 min.

Abstract:The antibacterial activities of twenty strains of endophytic fungi isolated from Eucommia ulmoides and their secondary metabolites were screened with Staphylococcus aureus,Bacillus subtilis and Escherichia coli. The results showed that there were fifteen

Abstract:对太白米的叶、大鳞茎和小鳞茎进行了化学成分系统预试，从大鳞茎中分离得到4个甾体生物碱苷，通过对其IR、FABMS、SIMS、1^HNMR、13^CNMR、DEPT、HMQC、HMBC和1^H-1^H COSY的综合解析鉴定了其中3个结构，它们分别为茄次碱-3-O-α-L-吡喃鼠李糖-(1→2)-β-D-吡喃葡萄糖苷(Ⅱ)、茄次碱-3-O-α-L-吡喃鼠李糖-(1→2)-[β-D-吡喃葡萄糖(1→4)]-β-D-吡喃葡萄糖苷(Ⅲ)和茄次碱-3-O-α-L-吡喃鼠李糖-(1→2)-[β-D-吡喃葡萄糖-(1→3)-β-D-吡喃葡萄糖(1→4)]-β-D-吡喃葡萄糖苷(IV)。

Abstract:采用反复柱色谱方法进行分离，通过理化性质和波谱分析鉴定结构。从土贝母(Bolbostemma paniculatum)分离并鉴定了8个化合物，分别为：麦芽酚(Ⅰ)、大黄素(Ⅱ)、△^7，22，25-豆甾三烯-3-醇(Ⅲ)、△^7，22，25-豆甾三烯醇-3-O-β-D-吡喃葡萄糖苷(Ⅳ)、α-羟基丙酬葡萄糖苷(V)、β-D-葡萄糖2→1 β-D-葡萄糖苷(Ⅵ)、胡萝卜苷(Ⅶ)、正三十一烷(Ⅷ)。化合物V～Ⅷ为首次从该属中分离得到。

Abstract:Mulberry red pigment is a safe nontoxic natural pigment.Eight types of macro-porous polymeric adsorbent were screened and then single-factor and orthogonal experiments and variance analysis were adopted to establish the optimal conditions for the adsorpti

Abstract:Lathyrus sativus L. seedlings were fully sprayed with Eu 3+ solution at certain concentrations and their physiological indexes concerned were measured after they had absorbed the solution.The results showed that in the roots of Eu 3+ solution sp

Abstract:利用连续25a在无石灰性潮土的定位施肥试验，研究了长期施肥对冬小麦品质及产量构成因素的影响，结果表明，高量有机肥配施高量氮肥、氮磷钾配施能提高冬小麦面粉吸水率、沉降值，延长面团形成时间、稳定时间和断裂时间，提高氨基酸总量和必需氨基酸含量。高量有机肥配施高量氮肥可提高冬小麦籽粒中谷氨酸、丝氨酸、脯氨酸、丙氨酸和苯丙氨酸的含量．增加37．21％～54．62％．与对照比较均达极显著水平。长期施肥对作物籽粒氨基酸含量的影响也不相同，冬小麦籽粒中氨基酸含量受施肥影响最大的是谷氨酸，变异系数(CV)为14％，最小的是苏氨酸和赖氨酸，CV为7．67％及5．27％。

Abstract:对不同水分条件下小麦叶片叶绿素含量、叶绿素荧光参数及光合速率的变化进行了研究．结果表明，随着胁迫的加剧．其叶绿素含量降低，且叶绿素a的含量下降比叶绿素b的含量要快。住不同水分条件下，叶片叶绿素含量及光合速率随生育进程的变化趋势基本一致．拔节期到抽穗期迅速上升，灌浆期后迅速下降，且叶绿素含量与光合速率呈显著的正相关。在水分胁迫下旗叶的T12值减少．旗叶光系统Ⅱ(PSⅡ)原初光能转化效率(Fv／Fm)和潜在活性(Fv／Fo)降低．胁迫程度越大其值降低幅度越大，轻度胁迫与对照相比其值降低幅度小。而重度胁迫其值急剧下降，这些值的降低，导致了光合作用的潜在活力降低，影响了光合电子的传递和CO2同化的正常进行，最终导致产量降低。

Abstract:利用砂培试验研究了不同氮素形态对向日葵生长和光合功能的影响。结果表明：NO^3-N(NN)处理的株高、叶面积、干物质重显著高于NH4^ -N(AA)处理，施用NO3^--N的植株有较高的净光合速率(Pn)和较低的气孔导度(C8)、细胞间隙CO2浓度(Ci)。不同处理之间Fv／Fm没有显著差异，NN处理的植株PSⅡ线性电子传递量子效率ФPSⅡ、光化学荧光猝灭系数qP和表观光合电子传递效率ETR都显著高于AA处理的植株；而非光化学荧光猝灭系数qN则明显低于AA处理的植株。碳同化受到抑制是NH4^ -N条件下净光合速率下降的重要原因。

Abstract:Folin procedure and ninhydrin method were adopted to determine the contents of soluble proteins and free amino acids in the cellular extravasations of Spirulina (Arthrospira) platensis (S_1) only found in the natron lakes of the Ordos Plateau,introduced S

Abstract:The fruits of the persimmon variety Jianshi (Diospyros kaki L. f. cv. Fuping Jianshi),planted in the Fuping Xian County,were used to study the firmness and the cell-wall components and their catabolic enzyme activities of post-harvest persimmon fruits at

Abstract:Under climatic conditions dry and hot river-valley regions of southwest China,which are typical of the south subtropical climate,four grapevine varieties,Merlot,Fox,Chardonnay and Gamay,began their physiological dormancy in early October,middle October,ea

Abstract:Anthesis period,scape length,perianth tube length,ovary length,fruit length,and seed and embryo length and width of G. elata Bl .f. elata,G. elata Bk.f.viridis Makino and G. elata Bl. f. glauca S. Chow were investigated in the flowering period and it was

Abstract:wheat varieties and germplasms from the wheat production regions located in the reaches of Yellow River and the Huaihe River were inoculated with individual races in the seedling period or with mixed races in te adult period to study their resistances to

Abstract:种群的分布格局与空间尺度有密切的关系.本研究将传统方法与点格局分析法结合起来,探讨了不同地区不同尺度下栓皮栎种群的空间分布.结果显示:随着样方面积的增大,种群的聚集度都有逐渐增大的趋势,当样方面积为10 0、2 0 0 m2 时,部分地区的丛生指标It、Cassie指数CA 小于零,当样方面积大于或等于4 0 0 m2 时,丛生指标It>0、Cassie指数CA>0、平均拥挤度m* >>1、L loyd聚块性指标和Morisita指数Iδ>1.用Morisita指数的随机分布偏离度的显著性F分布检验,当样方面积达到4 0 0 m2时,栓皮栎种群表现为聚集分布.同时,对不同地区不同样方面积上的栓皮栎种群进行Taylor和Iwao模型回归,Taylor回归系数b的变化从样方10 0、2 0 0 m2的b<1到4 0 0 m2的b>1,Iwao模型参数α、β值都表现为α>0 ,β>1,说明其分布格局主要表现为聚集分布.当样方面积为6 0 0、80 0 m2时,栓皮栎种群的空间格局呈现出随机分布.

Abstract:The Wawushan Mountain National forest Park is situated in the transforming area between the Qinghai-Tibetan Plateau and the Sichuan Basin.Its seed plants belong to 1 743 species,686 genera and 148 families.Its large families and genera play an important r

Abstract:Sycyos, a naturalized genus of cucurbitaceae in Mainland China, is reported in the paper.

Abstract:采用水蒸（蒸馏法和GC／MS方法对秦岭林区油松针叶挥发成分进行分析测定，鉴定出油松针叶中65种挥发性成分，其化学成分以萜烯类(79．39%)、酯类(9．16％)、醇类(10．36％)、醛类(0．25%)等为主，化合物则以α-蒎烯(5．92％)、β-蒎烯(6．09％)、乙酸龙脯酯(8．69%)、β-石竹烯(28．62%)、α-石竹烯(5．97%)、α-杜松醇(3．27％)、异杜松醇(3．44％)、萜烯醇(2．2j％)、杜松二烯(11．33%)为主。

Abstract:利用柱层析、薄层层析，从苦苣菜(Soncbus oleruceus)丙酮提取物中分离得到6个化合物，经1^HNMR、13^CNMR、IR等现代波谱技术鉴定，分别为洋芹素(1)、木犀草素(2)、槲皮素-3-O-葡萄糖苷(3)、3β-羟基-12-烯-乌苏酸(4)、对-甲氧基苯乙酸(5)、对-羟基苯乙酸(6)，这些化合物在该植物中为首次报道。

Abstract:The distribution of B-chromosome between Allium mairei diploids and tetraploids were investigated,and the activities of oxidation-resisting enzymes and the contents of malondialdebyde (MDA) and proline in the diploids at different altitudes were determine

Abstract:Jinyou 1,a cucumber variety,was used to study the effects of different soil water contents on the growth and physiological characteristics of greenhouse spring cucumber.The results indicated that with the content of soil water at 90% field water-holding c

Abstract:运用室内测定方法，对黄芪野生种子和栽培种子的吸不率及其不同处理下的种子发芽率进行了比较试验，结果表明，采用砂磨方法可使黄芪种子的萌发率明显高于其它方法，黄芪各子种皮的机械抵制是导致发芽率低的直接原因；黄芪种子在23℃/15℃变温度条件一的萌发率；不同采收期也会通过影响种子的成熟度而影响发芽率。

Abstract:The concept frame of phylogeography originated in the early researches of mitochondrial DNA (mt DNA).With the methods for phylogeography having advanced and become integrated with experimental techniques for molecular biology, molecular phylogeography has

Abstract:The ultraviolet (mainly UV-B) radiation enhanced by depleted ozone layer in stratosphere has caused considerable concern. Cyanobacterium use in studying the effects of UV-B on living organisms presents many advantages because of its importance in ecologic

Abstract:Environmental factors are always one of the major reasons why the symbiotic nitrogen-fixing system of rhizobia and legumes cannot come into full play in agricultural production. Now the environmental factors involved with the research include water, nutri

Abstract:Thirty three infrageneric names of Ephedra L. were reviewed.It is found that only 13 names are legitimate among the 33 infrageneric names of Ephedra,other 20 names include 19 invalid names and 1 later homonym.