Abstract:The germination and pollen tube growth of pear distant pollens on the stigma in 'Housui' and 'Osa-nijisseiki' were observed by fluorescence microscopy,which showed that(1) pear distant pollens could germinate on the stigmas but the germination rates diffe

Abstract:The anther development of the newly discovered male sterility line Sh-B in Salvia miltiorrhiza was anatomically examined on microscopic level and the pollen vigor and seed-setting percentage of the line were tested.It was shown that according to the morph

Abstract:The vegetative organ structures of Helleborus thibetanus Franch.were observed by anatomical method,which showed that the primary structure of H.thibetanus roots fell into the categories of triarch,tetrarch and hexarch.In the vegetative organs the vascular

Abstract:Light microscope and scanning electron microscope were used to examine micro-morphological features of the seeds of ten species belonging to six genera of tribe Anthemideae comparatively.It was shown that the seeds of the ten species were richly diverse i

Abstract:150,300 and 350 mmol/L NaCl and Na_2SO_4 were used to treat the seed-originated seedlings of Amygdalus communis L.and A.communis var.persiooides in greenhouse.Then the chlorophyll contents,net photosynthetic rates and stomatal conductance and intercellula

Abstract:The chloroplast numbers,morphologies and ultra-structures in the leaves of a xantha wheat mutants etiolating naturally and its parent(Xinong1718) were comparatively examined by transmission electron microscopy.It was shown that the mutant plants with thre

Abstract:在温室水培条件下对通辽杨(Populus simoniiCarr.×P.nigraL.‘Tongliao’)花粉母细胞减数分裂进程及其染色体行为进行了研究。结果表明:(1)通辽杨花粉母细胞减数分裂与其雄花芽/花序外部特征和花药颜色有着密切关系,前期Ⅰ作为减数分裂过程中最关键而复杂的一个阶段,大约占整个减数分裂进程90%的时间;中期Ⅰ存在单价体,后期Ⅰ有落后染色体出现,表现出较强的遗传杂合性,而且中期Ⅱ平行纺锤体的出现与天然花粉中大花粉的存在之间可能有着一定的联系;(2)在通辽杨减数分裂过程中核仁数目存在1~8个的动态变化,这种现象可能与杨属植物古多倍性起源有关,并推测通辽杨染色体组内至少存在8对带有次缢痕的染色体;(3)在同一个花芽,同一个小花,乃至同一个花药中,往往能同时观察到5~9个不同的分裂相,这种减数分裂不同步性是通辽杨适应环境的一种进化表现,对其种群繁殖具有重要意义。

Abstract:The growths of different ginkgo calluses were studied by determining the protein and flavonoids in three ginkgo calluses of one same origin,analyzing their protein in the three calluses by SDS-PAGE and observing the ultra-structures of the three calluses,

Abstract:Plant expression vector pCAMBIA1300-PaAFP was constructed by cloning PaAFP gene of P.americana to pCAMBIA1300,and then the engineered strain LBA4404 is constructed by transforming plant expression vector pCAMBIA1300-PaAFP into A.tumefaciens LBA4404,which

Abstract:TaNHX_2 was recombined to the downstream of CaMV35S promotor in plasmid pBIN438 to form binary phyto-expression vector pBIN438-TaNHX_2.pBIN438-TaNHX_2 was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated vacuum filtration and th

Abstract:‘长选3S’是由‘培矮64S’通过辐射诱变选育出的长穗颈温敏核不育水稻．以‘K选3s’二核花粉期穗颈节问作为目标群体，‘培矮64S’二核花粉期穗颈节间作为对照群体，进行抑制差减杂交，川经过‘培矮64S’cDNA差减的‘长选3S’cDNA构建了一个含有大约130个独立克隆的差减文库；采用差减前的‘培矮64S’cDNA和‘长选3S’cDNA以及正向／反向差减杂交后的cDNA为模板标记探针，对随机挑取的96个承组质粒进行羞示筛选，获得了20个阳性候选克隆。从这些阳性候选克隆中随机挑取了8个进行Northern blot分析．证实其巾中1个候选克隆代表了在‘长选3S’穗颈节间中特异表达的基因．序列分析和同源性比较表明它与信号传导有关。这一在‘长选3S’穗颈节间特异表达的cDNA片段有助于进一步揭示其穗颈节间伸长的分子机制．并为水稻不育系的遗传改良提供有用的素材。

Abstract:采用AFLP(Amplified fragment length polymorphism)技术对黄瓜(Cucumis sativusL.)品种‘津绿4号’的单倍体、二倍体和四倍体进行基因组DNA多态性比较。结果表明:(1)从35对引物扩增获得2 188条60~500 bp的条带,多态性位点仅有20个,占0.92%,其中22对引物组合扩增的不同倍性材料的AFLP指纹没有明显差异;(2)在多态性位点表现中,以二倍体的条带存在,单倍体和(或)四倍体的条带丢失为主,在四倍体中扩增出1条特异带;(3)与相应的二倍体相比,单倍体和四倍体有特异片段的消失和增加。

Abstract:Nine different cotton varieties with different maturities were arranged by partial diallel crossing to form 46 combinations and then the F_1 and F_2 of them were planted under different ecological environments to study seven traits relating to early matur

Abstract:Mature embryos of wheat as the explants were in vitro cultured in different pretreatments,at different 2,4-D and sucrose concentrations and in different KT combinations with 2,4-D to study the effects of these factors on callus induction and differentiati

Abstract:Hydroponics was adopted to study K~ ,Na~ and Cl~-contents in different parts of cucumber seedlings at different NaCl concentrations.It was shown that under NaCl stress cucumber plants decreased the K~ content and increased the Cl~-content and the decrem

Abstract:在温室采用砂培试验,比较研究了NaCl胁迫对2个菊芋(Helianthus tuberosusL.)品种‘南芋8号’(Ht 1,耐盐性较强)和‘南芋1号’(Ht 2,耐盐性较弱)幼苗生长及其离子吸收运输的影响。结果表明:(1)与对照相比,低浓度NaCl处理(255、0 mmol.L-1)促进了Ht 1根干物质积累和根冠比增加,有利于其养分吸收;较高浓度NaCl处理(1002、00 mmol.L-1)均显著降低2个品种的总干物质重以及叶片、茎、根干物质重,且Ht 2降幅较Ht 1大;(2)NaCl处理对菊芋幼苗根部相对含水量(RWC)没有显著影响,但对叶片和茎部RWC的影响较大,而且高浓度盐胁迫(200 mmol.L-1)下,耐盐品种Ht 1较Ht 2叶片能够保持较高的RWC;(3)NaCl处理下,Ht 1比Ht 2根部对K 的选择性较强,且由根部吸收的大量Na 和Cl-在运输过程中被区域化在茎部,因而向代谢活性较高的叶片运输量相对较少,这是Ht 1耐盐能力较强的一个重要原因。

Abstract:以秋冬季塑料大棚中盆栽西瓜幼苗为材料,研究了50~200 mg/L 5-氨基乙酰丙酸(ALA)处理对低温弱光条件下西瓜植株光合特性、碳水化合物积累、抗氧化酶活性和丙二醛(MDA)含量的影响。结果表明,外源ALA处理显著提高了西瓜幼苗叶片净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)。ALA处理还显著提高了叶片SOD和POD等抗氧化酶活性,但对CAT及APX活性影响不大,暗示ALA处理可能主要通过提高植株SOD和POD活性来促进西瓜幼苗活性氧代谢,从而保护细胞光合性能。处理后30 d分析表明,ALA处理植株干物质重量比对照高出42%~54%,淀粉含量增加62.2%~207.0%,可溶性糖含量也增加32.0%~87.1%。以上结果说明,ALA处理可以增强低温弱光条件下西瓜幼苗抗氧化系统活性,促进光合作用与光合产物积累,并促进植株生长。

Abstract:Wheat was hydroponically planted to study the characteristics of Cl~-uptake and accumulation of wheat and soil Cl~-movements in different soil water moistures at the seedling stage by Cl~-tracing.It was shown that(1) Cl~-accumulation had a close relation

Abstract:The water extracts of the aboveground parts of 31 plants belonging to 13 families were used in the seed germination experiment of the major weed Ligularia sagitata and the herbage Elymus nutans in alpine and cold grassland in order to screen the plants th

Abstract:在实验室通过模拟野外自然条件,研究了土壤水分、沙埋厚度和温度对黄柳种子发芽与幼苗生长的影响。结果表明:(1)黄柳种子对水分条件很敏感,发芽的适宜土壤含水量为3%~20%,低于3%或高于20%发芽都会受到抑制;(2)种子萌发需要沙土覆盖,适宜覆沙厚度为0.1~0.4 cm,此条件下第5天种子发芽率达80%以上;但当沙埋厚度超过0.6 cm时,种子出苗就会受到抑制;当沙埋厚度超过1.2 cm时,将不能出苗;(3)种子发芽的适宜温度为15~35℃,当温度高于45℃时种子将不能萌发并最终死亡;(4)幼苗的耐热性较强,40℃处理下幼苗出现热害症状,并且热害症状发展缓慢,60℃处理下90 min后幼苗干枯死亡;(5)种子的寿命为120 d左右。

Abstract:以乌兰布和沙漠东北部的甘蒙柽柳、沙木蓼、霸王、沙冬青、花棒、白刺、梭梭和柠条8种沙生灌木为材料,对沙生灌木生长季末期的光合生理特性进行了研究。结果表明:(1)8种沙生灌木秋季的气孔导度日变化有明显的峰谷;气孔导度日均值最大的为梭梭(0.71 mol.m-2.s-1),最小的为柠条(0.08 mol.m-2.s-1);(2)8种沙生灌木秋季的光合速率日变化均呈现单峰曲线,影响光合速率变化的因素以非气孔限制因素为主;光合速率峰值出现时间以柠条最早(11:30),甘蒙柽柳和沙木蓼最晚(15:30);光合速率日均值最大的为沙木蓼(7.99μmol.m-2.s-1),最小的为柠条(3.40μmol.m-2.s-1);(3)蒸腾速率的日变化除梭梭和柠条呈现单峰曲线外,其余6种灌木呈现出准双峰曲线或双峰曲线;蒸腾速率日均值最大的为梭梭(4.42 mmol.m-2.s-1),最小的为柠条(0.47mmol.m-2.s-1);气孔运动因素对蒸腾作用的影响占据优势;(4)8种沙生灌木的光合水分利用效率在白昼普遍高于早晚,且以柠条的光合水分利用效率日均值最大(7.18μmol.mmol-1),梭梭最小(1.23μmol.mmol-1);蒸腾速率日均值高的沙生灌木光合水分利用效率日均值低,反之亦然。

Abstract:应用2005年5~10月份EOS/MODIS资料,采用光能利用率估算方法,对中国西北荒漠绿洲区甘肃省安西县荒漠草原区的月净第一生产力、年净第一生产力进行估算,并结合地面观测,分析了不同牧草类型NPP的时间变化和空间分布特征。结果表明,安西县荒漠草原区2005年牧草生长期内NPP的变化基本是6、7月份最大,其它月份较小,5~6月份是牧草叶面积变化比较大的时期,也是NPP变化比较显著的时期;荒漠牧草对光的利用能力和对CO2同化能力有很大差异,白刺、红砂、黑果枸杞是荒漠草场净生产力相对比较大的牧草类型;荒漠牧草区年净第一生产力的空间分布有很大的差异,禁牧区牧草的年NPP相对非禁牧区的NPP较大,这一结论与地面观测结果相一致,NPP估算值平均误差为12.6%。

Abstract:Soil physical properties and leaf photosynthetic characteristics in three stands,Betula platyphylla forest,Quercus liaotungensis forest,and Betula platyphylla-Quercus liaotungensis mixed forest,which were located in the secondary zone of Ziwuling Forest o

Abstract:The structures and species diversity and their affecting factors of desert communities in Liangucheng Nature Reserve of Minqin County were studied in terms of species diversity index,forestland bearing capacity and soil water.It was shown that(1) in the c

Abstract:Mabian Dafengding National Nature Reserve is located in the limits of Mabian County,Sichuan and on the basis of field investigations and literatures references; the seed plant florae are studied. It is shown that there 1 240 species,446 genera and 120 fam

Abstract:Field investigations and data references indicates that there are totally 446 species(including varieties and forms),118 genera and 48 families of pteridophytes in Mt.Ailaoshan Nature Reserve.The dominant families are Dyopteridaceae,Polypodiaceae and Athy

Abstract:The paper lists 11 species and 4 varieties of Ranunculus plants in Qinling Mountains,which include four newly recorded species and three varieties,R.pulchellus C.A.Mey.,R.dielsianus var.suprasericeus Handel-Mazzetti,R.tanguticus var.dasycarpus(Maxim.) L.L

Abstract:The study investigated the yield-compositions of winter wheat and chlorophyll contents,MDA and SOD contents in the flag leaves and water use efficiencies of winter wheat at the kernel-filling stage under the farming practices adopting five modes of moistu

Abstract:Six genotypes in three generations,cabbage F_1 and F_2 and inbred S_3 were used in anther culture to study the factors affecting embryoid induction and formation of cabbage anthers in culture.It was shown that(1) high sucrose concentration exerted signifi

Abstract:The content of aloe-emodin,rhein,emodin,chrysophanoland physcion in 3 and 4 year old cultivated Rheum tanguticum ,which grew in Qinghai in different months of it growth season,were determined by high performance liquid chromatography. It was shown that in

Abstract:采用纤维素酶法提取杜仲叶中的主要药效成分———苯丙素类的绿原酸(CHA),通过单因素试验、正交试验和方差分析确定了纤维素酶法提取杜仲叶中绿原酸的最佳操作条件。结果表明,加入550 U/g纤维素酶0.50%,pH 4.5,温度40℃,提取率最高可达到51.84 mg/g。

Abstract:In plants,plasmalemma H~ -ATPase,participating in substance trans-membrane transport and elongation growth of the cells,the opening and closedown of the stomata and the responses to environmental stress,is a master enzyme.The regulation of its activity in

Abstract:Binary Ti vectors are the most important plant-transforming vector in plant genetic engineering.In the recent years,there a quick development in binary Ti vectors,which continuously results in new binary Ti vectors.The newly developed binary Ti vectors ha

Abstract:K~ plays an important role in life of plants.K~ transporters are generally composed of high-affinity and low-affinity two part.On molecular level,high-affinity uptake are mainly carried out by K~ transporters belonging to KUP/HAK/KT and HKT families.K~