Abstract:The stems of eights mosses growing in extreme habits(xeric and hydric habitats),Hedwigia ciliata(Hedw.) P.Beauv.,Andreaea rupestris Hedw.var.fauriei(Besch) Takaki,Grimmia macrotheca Mitt.,Macromitrium japonicum Doz.et Molk and Racomitrium aquaticum(Schrqa

Abstract:用JEM-100CXⅡ透射电子显微镜对宁夏枸杞(Ly cium barbarum L.)花粉发育过程做了超微结构观察.结果表明,在花粉母细胞细线和偶线期,核糖体数量减少、线粒体结构简化;之后核糖体数量逐渐回升,后期Ⅰ线粒体结构恢复正常.小孢子液泡化过程中核糖体再次减少,同时线粒体和质体结构简化;在早期二胞花粉中,核糖体数量增加、线粒体和质体结构再度分化.花粉母细胞和小孢子发育过程中都存在“细胞质改组”现象,且这两次“细胞质改组”与细胞功能的转变密切相关.在次生造孢细胞、花粉母细胞和小孢子后期都有液泡数量明显增加或体积增大的过程.前两次液泡的增加可能参与了其胼胝质壁的构建;而小孢子晚期中的大液泡则创造了一种极性为其不等分裂作好了生理和结构上的准备.

Abstract:用焦锑酸钾沉淀法对45℃和28℃下黄瓜(新泰密刺)授粉后柱头及子房中C a2 分布进行了电镜观察,并采用酶联免疫吸附测定法(EL ISA)测定了叶片中的ABA含量及用等电聚焦聚丙烯酰胺双向电泳(IEF-SDS PAGE)方法对其蛋白合成的变化进行了研究.结果表明,28℃时柱头中的C a2 主要分布在细胞间隙中,经45℃处理后细胞间隙和胞内C a2 水平均显著升高,胞内外C a2 浓度梯度逐步丧失;45℃处理1 h后柱头和子房中内源ABA含量显著提高;高温处理后柱头和子房中产生了一些新的蛋白质,如柱头中的(MW26.0 kD,P I 5.4)、(MW90.0 kD,P I5.1)、(MW54.0 kD,P I 4.6)、(MW41.0 kD,P I 6.2),子房中的(MW90.0 kD,P I5.2)、(MW61.0 kD,P I 5.4)、(MW48.0 kD,P I 6.4)、(MW40.5 kD,P I 4.9);另有一些蛋白质与常温相比表达量大幅上调,如柱头中的(MW67.0kD,P I 5.8)、(MW56.5 kD,P I 5.8),子房中的(MW70.0 kD,P I 5.7)、(MW57.0 kD,P I 5.7).上述结果表明,C a2 和ABA信号系统均参与了授粉后黄瓜雌性器官对高温胁迫的反应调节并最终导致基因表达发生变化.

Abstract:Changchun mici' and 'Beijingjietou',two cucumber varieties remarkably differing in cold tolerance,were used to study the effects of Ca~(2 ),EGTA,a Ca~(2 ) chelator,CPZ,a Calmodulin antagonist,on the activities of three anti-oxidative enzymes and POD isoen

Abstract:The study probed the relations of the net photosynthetic rates(Pn),apparent quantum yields(AQY),stomatal conductance(Gs),transpiration rates(Tr) and intercellular CO_2 concentrations(Ci) with the photosynthetic flux densities(PFD) of grafted and own-root

Abstract:Potaninia mongolica Maxim, a species of the monotypic genus Potaninia, belongs to the family Rosaceae. It is well known for its tripetalous flowers distinct in the Rose Family, with sepals 3,petals 3 and stamens 3. Stems branched, with persistent ,hard an

Abstract:在100 mm o l/L N aC l胁迫下,研究了外源多胺-腐胺(Pu t)、尸胺(C ad)、亚精胺(Spd)和精胺(Spm)对玉米幼苗生长、光合速率和PSⅡ光化学效率(Fv/Fm)、叶绿体结合多胺和叶片丙二醛(M DA)含量以及抗氧化酶活性的影响.结果表明,叶面喷施1 mm o l/L的Pu t、Spd和Spm可显著增加盐胁迫下玉米幼苗干物质重、叶绿体内结合态多胺的含量、叶片净光合速率和PSⅡ光化学效率(Fv/Fm),并降低了叶片中M DA含量.外源Spd和Spm明显增加盐胁迫下玉米幼苗的抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)和过氧化氢酶(CAT)活性;外源Pu t可增强APX和POD活性,对CAT活性的影响不明显.这些结果表明多胺对玉米盐害的缓解作用可能是由于提高了叶绿体中结合态多胺的含量和叶片抗氧化酶的活性,从而增强了盐胁迫下的玉米光合能力.

Abstract:用PAM 2000型便携式荧光测定系统测定了不同浓度N arc iclas ine(NCS)处理的离体萝卜子叶的荧光动力学参数:初始荧光(F0)、光化学淬灭(qP)、光系统Ⅱ最大光化学效率(Fv/Fm)、光合电子传递速率(ETR).结果表明,NCS对各种叶绿素荧光参数均显示出明显的抑制作用,而且NCS的抑制作用随其浓度的增加而逐渐增强;利用蓝绿温和胶电泳技术分析萝卜子叶类囊体膜蛋白复合物发现,转绿期间叶绿体中光系统Ⅱ(PSⅡ)和光系统捕光色素蛋白复合体的含量增加均被NCS抑制.表明从水仙鳞茎分泌物中分离提取的生物活性物质NCS能够明显抑制离体萝卜子叶的光下转绿,而且NCS对离体萝卜子叶光下转绿的抑制是多位点的.

Abstract:以2年生三叶漆(T erm inth ia p an icu la ta)幼苗为实验材料,研究了生长于全自然光和遮荫条件(光强相当于自然光强的50%)下幼苗的光合特性和叶绿素荧光参数对不同土壤水分条件的响应,探讨了其对干旱和强光胁迫的生理适应机制.结果表明,叶片相对含水量(RW C)、叶水势(Ψ)、最大光合速率(Pm ax)、表观量子效率(AQY)、暗呼吸速率(Rd)和光合色素含量都随干旱胁迫的加剧而下降.Pm ax、AQY、Rd和比叶重(LM A)随光强的增加而升高,色素含量则随光强升高而降低.Ch la/b、C ar/Ch l随干旱程度和光强的增加有升高的趋势.PSⅡ的最大光能转换效率(Fv/Fm)和光化学量子效率(ФPSⅡ)在日间光较强时明显降低,说明发生了光抑制.电子传递速率(ETR)和非光化学猝灭系数(N PQ)随日间光强的增大而升高,表明三叶漆可能通过增强光呼吸和热耗散抵御光抑制、保护光合机构.二元方差分析表明,水分和光强具有明显的交互作用.全自然光下严重干旱的幼苗仍有较高的Pm ax(9.65μm o l.m-2.-s 1),说明三叶漆对干旱和强光具有极强的适应能力,这也是其成为干热河谷植被优势种的重要原因.

Abstract:用荧光淬灭分析技术研究了断根对冬小麦荧光特性的影响以及不同水分条件下断根对产量的影响.结果表明,在不同生育期中小麦断根与未断根处理相比其最大量子产量(Fv/Fo)和PSⅡ潜在活性(Fv/Fm)无显著差异.通过对不同生育期荧光特性研究表明,断根初期小麦电子传递速率和有效光量子产量降低,而PSⅡ光合电子传递活性增加,孕穗期随着更多的能量向地上部的运转,此时根系补偿作用使ETR、ΦPSⅡ和qP增加,而且断根并显著降低光系统Ⅱ光化学效率.混播处理ΦPSⅡ和ETR值高于单播处理,认为断根降低了小麦的竞争能力,使总体光合活性增强,促进了能量向地上部运转,与混播处理产量增加相一致.小麦单播湿润处理下,断根降低了小麦穗重和地上部生物量,且籽粒产量降低了19.2%.在干旱处理下,断根降低了根冠比,提高了小麦穗重和籽粒产量.

Abstract:在6种不同浓度的铈(C e3 )对0.1 m g.L-1的Cu2 毒害下,研究了菹草叶片中保护酶SOD、POD、CAT的活性,活性氧H2O2,膜脂过氧化产物M DA含量及叶绿素含量等的变化及影响.结果表明,在9~12 d之内,7.5 m g.L-1以下的C e3 可以增强SOD、CAT、POD活性,降低M DA的含量,提高叶片中叶绿素和可溶性蛋白含量,从而减轻Cu2 对菹草植物体的伤害.而随着C e3 作用时间的延长和浓度的增大,C e3 的缓解作用不断减弱,C e3 和Cu2 产生协同效应,加重毒害.本实验结果认为,5~7.5 m g.L-1的C e3 缓解菹草叶片Cu2 毒害效果最好.

Abstract:Lignin contents and the activities of the enzymes related to lignin biosynthesis in the stocks and scions of watermelon after grafting were studied.The results showed that lignin biosynthesis accelerated in the stock and scions after grafting and POD,CAT,

Abstract:The progenies of the crosses of Cucumis species with different ploidities [allotetraploid C.hytivus(2n=3x=26,HHCC),allotriploid(2n=3x=26,HCC) and reciprocal F_1 hybirds(2n=2x=19,HC/CH)] and their parental materials were comparatively examined to study the

Abstract:F_1 plants of the direct cross of 'Qinguan'(white flesh) and 'Shuguang'(yellow flesh),two nectarine varieties(Prunus persica L.var.nectarina),was used at the materials to screen by RAPD and BSA(Bulk segregate analysis) the molecular markers closely linkin

Abstract:Narcissus monosomes with middle centromeres were isolated with glass needles with the help of micromanipulator and then put into 0. 2 mL Eppendorf tubes;next ,the monosomes were deproteinised and digested with Sau3A;afterwards,the DNA fragments of the mon

Abstract:为进一步研究干旱胁迫下小麦硫转运蛋白基因(ST)的表达,选用小麦-αT ubu lin基因(T a)为内参照,提取小麦根系总RNA,反转录cDNA,同批异管对2个基因片段进行PCR扩增.通过对PCR体系中M g2 浓度、退火温度及循环次数的优化和对体系重复性、准确性的分析,最终建立了一个稳定、方便的半定量RT-PCR体系.该体系可用于比较不同小麦样品间硫转运蛋白基因的表达,且因为两对引物均垮内含子,其稳定RT-PCR体系,也为实验室建立小麦mRNA反转录体系提供了参考依据.

Abstract:Extracting high-quality RNA from plant tissue is the prerequisite and key in carrying out researches in molecular biology.Because different tissues of same plant species differ in component composition,RNA extraction methods have different nodi.A method o

Abstract:Different Arctium lappa L.explants and the compounding proportions of different hormones were comparatively studied in tissue culture and thereafter a high-efficiency plant-regeneration system by in vitro A.lappa culture.On MS mediums containing 2.0 mg/L

Abstract:为了在小麦品质育种中充分利用品种资源,以引进的57份小麦品种(系)为试验材料,采用SDS-PAGE和单籽粒硬度仪(SKCS)分析了这些品种(系)的高分子量谷蛋白亚基(HMW-G S)组成及其籽粒硬度.共检测到13种亚基和21种亚基组合,30份材料具有5 10亚基,10份2*,9份17 18,1份13 16.5 10和2*在硬质麦中出现的频率较混合麦高,在软质麦中的频率最低,17 18在混合麦中的频率较高.HMW-G S组合中,N u ll、7 9、2 12和1、7 8、2 12的频率较高,分别为17.5%和14.0%,个别品种还同时聚合有1A、1B、1D上的优质亚基.参试品种(系)含硬质麦32份(1级20份、2级12份),混合麦15份(2级3份,3级12份),软质麦10份(4级6份,5级4份),籽粒硬度的分布范围为12~74.春小麦和冬小麦材料N e i s平均遗传变异系数分别为0.550 8和0.573 3,表明春小麦的高分子量谷蛋白位点的遗传变异略低于冬小麦;春小麦和冬小麦A、B和D基因组的N e i s平均遗传变异系数分别为0.497 5、0.648 7和0.540 3,说明G lu-B 1位点的遗传多样性最高,其次是G lu-D 1位点,G lu-A 1位点最低.

Abstract:慢白粉品种‘百农64’与感病品种‘京双16’杂交,F1自交并分别与两亲本回交,获得包括亲本在内的6个世代,分析了‘百农64’慢白粉性的基因数目、遗传力和遗传模型.结果表明,‘百农64’慢白粉性受3对基因控制,其中2对基因的显性作用较强,另1对基因的显性作用较弱;广义遗传力为0.673 0±0.015 8,狭义遗传力为0.299 8±0.132 2;遗传方式符合加性-显性模型.

Abstract:

Abstract:A field experiment was carried out with eight winter wheat varieties,NR 9405,9430, Yanshi 9, Xiaoyan 6,Xinong 2208 and Shaan 229,Aifeng 3 and Shang 188,and Eum-Orthic anthrosols for studying the contributions of the leaves of wheat at different positions

Abstract:在盆栽条件下,研究了3种染色体倍性小麦的叶绿素荧光参数受水分亏缺的影响.结果表明,水分亏缺下3种小麦灌浆期旗叶的荧光参数表现为F v/Fm(可变荧光与最大荧光比)、qP(光化学猝灭系数)、ETR(表观光电子传递速率)下降,qN P(非光化学猝灭系数)上升,导致3种小麦的光合速率下降,其中四倍体小麦的这些光合参数受影响最大.试验还发现正常供水下小麦随倍性增大光合速率降低,而qP升高,qN P下降,由此推断小麦从二倍体向四倍体、六倍体进化的过程中,PSⅡ(光系统Ⅱ)天线色素吸收的光能用于光化学反应的份额变大,PSⅡ反应中心非辐射能量耗散能力降低,导致用于光化学反应的光能更容易过量,造成光合机构损伤,引发光抑制,这有可能是小麦在进化中光合速率降低的一个原因.

Abstract:With the data collected by nearly 80 meteorogical stations and the data of major summer and autumn grain crops during their growth of Gansu province in 1961~2000,the paper combines Penman-Monteith formulae to calculate the water requirements of major summ

Abstract:The sap-flows of main stems and primary and secondary shoots of Hedysarum scoparium Fisch.et Mey.were examined by Stem heat balance(SHB),which showed that the rates of the sap flows daily varied without following a certain pattern during the whole growing

Abstract:Since 1998,more than samples of 36 species,14 genera and 4 families of herbages had been introduced one after another to enrich the germplasms for the hilly and gully regions of the Loess Plateau and provide technical supports to the transformation of far

Abstract:Young liquorice(Glycyrrhiza uralensis) seedlings in five different habitats were used for investigating their POD,EST,LDH and MDH isoenzymes by SDS-PAGE and conducting their water stress experiment with PEG 6000;In the meantime,aboveground morphologies of

Abstract:The study probed the changes of alpine meadows in vegetation composition,diversity,functional group and productivity.The results indicated that as the intensity of sward cleavage increased,Elymus nutans and other plants that had high requirements in soil

Abstract:通过多年野外考查,在山西运城盐池湖区采集到蓝藻共17属35种(含变种),其中色球藻科9属19种,胶须藻科1属1种,颤藻科4属12种,念珠藻科3属3种.该区域的水体根据其含盐量的不同可分为4种类型:淡水水体(含盐量0.001%~0.05%)、混盐水体(含盐量0.05%~3%)、真盐水体(含盐量3%~4%)和高盐水体(含盐量4%~34.7%).受含盐量的影响,蓝藻在各类水体中的分布有明显差异,随含盐量增加,种类数减少,在高盐水体未见分布.与国内其它3个盐湖藻类的分布相比较,蓝藻在混盐水体比淡水水体具有更高的相似性.

Abstract:分别应用基于二元属性数据的群落相异性系数、Cody指数、W ilson和Shm ida指数及Sorenson指数和基于数量数据的B ray-Curtis指数对五台山亚高山草甸的β多样性沿海拔梯度的变化规律进行了研究.结果表明:群落相异性系数、Cody指数、W ilson和Shm ida指数总体上随海拔升高呈现相似的变化趋势,但波动幅度有一定差异.B ray-Curtis指数对群落沿海拔梯度的变化更为敏感,它所反映的结果更准确可靠.同时,除海拔梯度外,人为活动干扰也是引起β多样性发生变化的一个不可忽视的重要因素.

Abstract:The paper discusses the vegetation characteristics of the forests in Yinnashan Mountain Nature Reserve putting emphasis on their vertical distribution patterns and the results show that the vegetations present a clear vertical structural distribution with

Abstract:The broth of Cordyceps militaris fermentation in an oscillating manner were extracted with EtOAa and n-BuOH and the anti-microbial activity of the extracts were tested. The results showed that the extract by EtOAc presented a significant effect on Staphyl

Abstract:An open-top chamber were employed to treat two-year-old saplings of ten landscape tree species with sulfur dioxide at different concentrations and the gas-exchanging parameters of the leaves of the saplings were measured with TSP-1 Infra-red CO_2 analytic

Abstract:从细穗玄参(S crof ella ch inensisM ax im.)全草的正丁醇提取物中分离并鉴定了6个化合物,利用现代波谱技术(M S1、H NM R1、3C NM R、DEPT、HM BC、HM QC)和化学方法进行结构鉴定,分别为6-O-(2″-对羟基反式肉桂酸-α-L-鼠李糖)梓醇(1)、山栀子苷(2)、桃叶珊瑚苷(3)、玉叶金花苷酸(4)、熊果苷(5)、肌氨酸乙酯(6).6个化合物均为首次从该植物中发现.

Abstract:5月中旬选取典型晴天用L I-6400便携式光合仪对藜从第一片完全展开叶倒数第3~5片功能叶进行有关光合参数的测定.结果表明:藜的光合日变化呈双峰型,有明显的“午休”现象,第1个峰值出现在8:30左右,P n达到9.34μm o l CO2.m-2.s-1;第2个峰值出现在16:00左右,P n为4.26μm o l CO2.m-2.-s 1.造成光合午休的主要原因是受气孔调节.在400μm o l.m o-l 1CO2浓度下藜的光饱和点(LSP)为1 350μm o l CO2.m-2.-s 1左右,光补偿点(LCP)为22.5μm o l CO2.m-2.-s 1左右;表观量子效率(AQY)为0.035μm o l CO2.m o-l 1photons.二氧化碳饱和点(CSP)在976μm o l CO2.m o-l 1左右,二氧化碳补偿点(CCP)在40μm o l CO2.m o-l 1左右,羧化效率(CE)为0.113 2μm o l CO2.m-2.-s 1.说明藜是一种具有较强耐荫能力的C3型阳性植物.

Abstract:Antimicrobial peptides of plant origins is a type of low molecular weight peptides that plants synthesize to defend against the invasion of environmental microbes and most of them are cationic peptides and has good thermal stability.According to their act

Abstract:More and more evidences showed that H_2O_2 as signal molecule plays an important role inside plant bodies.It is the key factor regulating programmed cell death in pathogen,evocator and hormone responses.H_2O_2 has also been proved to play the signal role