Abstract:AtSTP3 promoter,which expressed specifically in green tissues,was isolated from Arabidopsis thaliana of Colombia type By PCR and its sequencing indicated that the amplified band (1 744 bp) was 99.9% homologous to the reported ones at the correspondent seq

Abstract:GISH and SSR were adopted to genetically analyze 15 offspring plants (lines) of the new wheat germplasm 4844 with large and multiflorous spikes.It was found that 4844-12 was a stable alien additional line and 4844-2 and 4844-8 were stable alien substituti

Abstract:108 F_2 plants in the cross of two cotton varieties,Bar19/1 and Acala 1517-77 were chosen to construct the genetic map,which had a whole length of 814 cM and covered 18.3% of the geneome of cotton,with 85 markers (70 SSR markers and 15 AFLP markers).The m

Abstract:Salt tolerance-related genes in Bruguiera sexangula were transformed into Oryza sativa subsp.japonica cv.Nipponbare,a keng-rice variety.The optimal system of the transformation was found by detecting the callus-transformation rate with GUS gene to be the

Abstract:According to the reported conservative sequences of Pinb gene,a pair of specific primers were designed to amplify,clone and sequence Pinb gene of DNA genome in Aegilops bicornis (SS) and a new allele of Pinb was found.The allele was 360 bp long,encoded 11

Abstract:以小麦条锈菌(Puccinia striiformis f.sp.tritici)野生毒性菌株为转化受体,以含有gus报告基因的质粒(pGUS6L20)和潮霉素抗性基因的质粒(pKLHyg14)为载体,应用基因枪法研究了小麦条锈菌夏孢子遗传转化的瞬时表达特征。结果表明,在金粉直径为0.6μm、射程6cm、载体DNA5μL、可裂膜压力为900Psi或1100Psi时,gus基因和潮霉素抗性基因的瞬时表达率相对较高。

Abstract:The genetic differences of 59 flue-cured tobacco varieties were examined by retrotransposon-based IRAP markers.The results showed that 151 bands were amplified with five primers designed according to the sequences of the retrotransposons Tnt1,Tto1 and Tnd

Abstract:利用RAPD技术,从130个随机引物中筛选出46个引物,对欧洲甜樱桃、欧洲酸樱桃、马哈利樱桃和野生中国樱桃4个类型樱桃种,以及欧洲甜樱桃与中国樱桃的种间杂交种共15个品种的基因组遗传变异进行分析。结果表明,46个随机引物均得到了稳定可重复的RAPD图谱,扩增出的DNA条带大小在100~2625bp之间,多态性位点数517个,多态性位点百分率为98.85%,每个随机引物扩增出的多态性DNA条带数在4~23条。品种间Nei遗传距离在0.166~0.479之间,平均遗传距离0.329;甜樱桃新品种‘秦樱1号’与‘秦岭玛瑙’、‘CDR-1’等10个樱桃砧木之间的遗传距离在0.248~0.376,并且根据遗传距离可以相互区分,所分析的15个樱桃品种均扩增出了特有的DNA条带,每个樱桃特有标记带在2~17个之间,共扩增出149个特有标记,据此可以进行樱桃品种及砧木的RAPD鉴定。研究认为利用RAPD技术可以在分子水平上对甜樱桃品种及其砧木进行快速鉴定。

Abstract:RAPD was adopted to examine genetic diversity of Petrosimonia sibirica in Oasis-desert Transitional Area of Fukang,Xinjiang and ORIGIN application was used to conduct the regression analysis of genetic diversity and soil factors of P.sibirica.It was shown

Abstract:The cotyledons,hypocotyls and roots of \?rpesium macrocephalum\% Franch.et Sav. seedlings were used as the explants to study callus induction and proliferation of \%C.macrocephalum\%.The results indicated that the roots of aseptic \%C.macrocephalum\% seed

Abstract:以苦荞子叶和下胚轴为外植体,进行了不同浓度激素组合的MS和SH固体培养基对胚性愈伤组织诱导及植株再生的研究。结果发现,MS培养基比SH培养基更有利于胚性愈伤组织诱导;2,4-D是诱导愈伤组织的有效激素,KT能有效促进胚状体的形成;下胚轴和子叶都能有效诱导出胚性愈伤组织和再生植株。下胚轴在MS 1.5mg·L-12,4-D 1.5mg·L-1BA培养基,子叶在MS 2mg·L-12,4-D 0.5~1.5mg·L-1BA上能高效诱导出愈伤组织;愈伤组织在MS 2mg·L-12,4-D 0.1mg·L-1KT培养基中继代,能有效诱导胚性愈伤组织;来自下胚轴的胚性愈伤组织在1/2MS 2.0mg·L-1BA 0.5mg·L-1KT 0.1mg·L-1NAA培养基上能够高频再生出芽,来自子叶的胚性愈伤组织在1/2MS 1.0mg·L-1BA 0.1mg·L-1KT 0.1mg·L-1NAA培养基上芽诱导率较高;MS 1mg·L-1NAA是适宜的再生苗生根培养基。

Abstract:The microsporogenesis processes of genetic male sterility line and its allele fertile line in \%Brassica napus\% L.were anatomically observed with Olympus BH\-2 microscope and it was found that in the regular microspore development,the tapetum disintegrat

Abstract:The pollen morphologies of one variety and 18 species belonging to 11 genera of Lactucinae Subtribe in Shandong were examined by light microscopy and scanning electron microscopy.The results showed that the pollens of Lactucinae Subtribe appeared globular

Abstract:The contents of major soluble sugars and the activities of sugar metabolism-related enzymes at different developmental stages in Cistanche deserticola and its host Haloxylon ammodendron were determined to study sugar metabolisms and their relations of C.d

Abstract:对6个不结球白菜品种的光合作用特性进行了研究。结果表明,在800μmol·m-2·s-1的光强下,不结球白菜的净光合速率以‘正大抗热青3号’最高,达16.37μmol CO2·m-2·s-1,其光合作用表观量子效率、羧化效率和水分利用效率也最高,分别为0.0442、0.0854mol·m-2·s-1和6.20μmol CO2·mmol-1H2O;暗呼吸速率以‘绿星’最低,为2.24μmol CO2·m-2·s-1;Pn-PFD响应曲线显示,在光强300μmol·m-2·s-1以下,各品种的净光合速率差异较小,在光强为300~1000μmol·m-2·s-1区段时,净光合速率随着光照强度增加而迅速增加。‘正大抗热青3号’光饱和点最高,达1910.3μmol·m-2·s-1,其光饱和点的净光合速率也最高,达20.2μmol CO2·m-2·s-1;不结球白菜不同品种净光合速率日变化进程相似,不论数值高低均呈双峰曲线型,有明显的“午休”现象。

Abstract:Three spring wheat varieties were hydroponically cultured to study morphological and physiological characters of the roots of different spring wheat varieties under low-potassium stress and the results showed that (1) different spring wheat varieties sign

Abstract:The effects of NaCl stress on the contents of the substances regulating membrane lipid oxidation and osmosis and photosynthetic characteristics of grafted and scion-root plants and scion-root plants of cucumber were studied with King shintosa,a salt-toler

Abstract:通过4个水平的砂培试验研究了外源氯对库拉索芦荟(Aloe vera)生长和品质的影响。结果表明:(1)根系相对干质量的含水量、植株干质量、根冠比分别在施氯浓度≥25mmol·L-1、≥50mmol·L-1、≥100mmol·L-1时显著下降(P<0.05);(2)外源氯胁迫下,Cl-主要在芦荟根部积累,且外源氯浓度越高,根系Cl-含量越多而叶片的Cl-含量越少;(3)硝酸盐主要分布在根系和功能叶(5叶)上,施氯≤50mmol·L-1时叶片和根系的硝酸盐含量都显著低于对照;(4)随氯浓度水平的升高,蒽醌和可溶性蛋白含量均显著增加,游离氨基酸含量变化不大,芦荟甙含量在施氯≤50mmol·L时显著下降。

Abstract:以2个耐盐性不同的番茄品种为材料,研究了外源NO供体硝普钠(SNP)处理对100mmol·L-1NaCl胁迫下番茄幼苗生长和光合作用的影响。结果表明:外源NO能使盐胁迫下的番茄幼苗叶片叶绿素含量、净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)升高,胞间CO2浓度(Ci)下降,叶绿素荧光参数Fv/Fm、Fv/Fo和T1/2增高,脯氨酸和可溶性糖含量升高。可见,外源NO有利于番茄幼苗对光能的捕获和转换,促进番茄的生长,降低盐胁迫对番茄的抑制作用。

Abstract:Corn pollens were collected at the pollinating stage of corn,then treated with enhanced UV-B radiation and used in artificial pollination.The results showed that as the UV-B radiation prolonged,the SOD,POD and CAT activities in the pollens appeared to dec

Abstract:A hydroponic experiment was conducted to study the effects of sulfur and organic fertilizer fertilization on carbon and nitrogen transport in winter wheat under copper and cadmium stresses.The results showed that under copper and cadmium stresses sulfur a

Abstract:采用微量点滴法和载毒叶片饲喂法测定30种药用植物乙醇提取物对粘虫3龄幼虫的触杀、胃毒以及拒食活性。结果表明,供试样品在50mg·mL-1浓度下均无明显的触杀或胃毒作用,但卡瓦胡椒和川陈皮对试虫有一定的拒食活性,其中卡瓦胡椒24h拒食中浓(AFC50)为4.12mg·mL-1,48h为9.12mg·mL-1。采用抑制菌丝生长速率法和抑制孢子萌发法测定离体杀菌活性的结果表明,供试样品在2mg·mL-1浓度下,卡瓦胡椒和厚朴对供试病原菌均有较强抑制作用。毒力测定结果表明,卡瓦胡椒对玉米大斑病菌、番茄灰霉病菌、小麦根腐病菌、油菜菌核病菌4种病原真菌菌丝生长抑制的有效中浓(EC50)分别为108、258、290、205mg·L-1,厚朴分别为208、331、345、408mg·L-1;卡瓦胡椒对玉米大斑病菌、玉米小斑病菌、烟草赤星病菌3种病原真菌抑制孢子萌发的有效中浓(EC50)分别为155、195、268mg·L-1,厚朴则分别为151、242、241mg·L-1。根据以上结果,我们认为卡瓦胡椒和厚朴的乙醇提取物具有较高的杀菌活性,其有效成分值得进一步研究。

Abstract:Descurainia sophia seeds were defatted with petroleum ether and then their glucosinolates were extracted with boiling water.The glucosinolates extract were purified with the column filled with DEAE sephadex A-25 and the molecular structures of its major g

Abstract:Based on the classification of desert vegetations in Xinjiang,the paper employs the Holdridge life zone model to simulate the desert vegetation types in Xinjiang and adopts Kappa coefficient to verify the simulation finding that it performs poorly (Kappa

Abstract:陕西省青木川自然保护区有种子植物905种,隶属于126科501属,其中裸子植物5科11属15种,被子植物121科490属890种(双子叶植物108科401属762种,单子叶植物13科89属128种)。中国特有属20属,中国特有种261种。该区系的基本特征是:植物种类丰富,特有程度高;区系成分复杂,表现出亚热带向温带过渡的特点,并以温带区系成分占优势;在中国植物区系上处于中国-日本森林植物亚区的华中地区和华北地区的黄土高原地区与中国-喜马拉雅森林植物亚区的横断山脉地区的交汇地带,多种区系成分汇集,与周邻联系广泛;植物区系起源古老,有较多的残遗植物;珍稀保护物种丰富,共计有《国家重点保护野生植物名录》级保护植物4种,级25种。

Abstract:Two newly recorded fern species from Qinling,Shaanxi,are reported,namely Polystichum melanostipes and Dryopteris pycnopteroides.Based on the specimens,we elaborated the morphology of the two species and discussed their geographical distribution.

Abstract:In this paper,three newly recorded species of the genus Lepraria from China are reported,which are Lepraria lobificans Nyl.,L.eburnea J.R.Laundon and L.caesioalba (B.de Lesd.) J.R.Laundon.The general data on habitats,distinguishable characteristics and ge

Abstract:Based on study of herbarium specimens and published materials on Hedysarum,present paper confirms the occurrence of Hedysarum austrosibiricum B.Fedtsch.in China and H.fruticosum Pall.in Xinjiang Province of China.In addition,cultivation reality of H.coron

Abstract:Steam distillation was adopted to extract the essential oil from Hypericum perforatum L.(whole plants),a Chinese medicinal herb.75 chemical compounds of the essential oil were identified and determined by GC/MS and of the compounds thymol,eucalyptol and e

Abstract:Polysaccharides are one major active ingredient.This study determined the root polysaccharides contents of six varieties of \%Astragalus membranaceus\% and \%A.membranaceus\% var.\%mongolicus\% at different growth stages and their root polysaccharide and

Abstract:以南芋2号为材料,设计1/2Hoagland(H)、1/2H 10mmol·L-1CaCl2、1/2H 30%海水、1/2H 30%海水 10mmol·L-1CaCl2、1/2H 30%海水 5mmol·L-1EGTA5个处理水平,研究了Ca2 对海水胁迫下菊芋鲜重以及菊芋叶片中MDA含量、相对电导率、叶绿素含量和净光合速率(Pn)的影响,以探索Ca2 对缓解植物海水胁迫作用机制。结果表明:正常生长条件下外施10mmol·L-1Ca2 对菊芋的生长没有显著影响;在1/2H 30%海水处理下,菊芋的正常生理代谢明显受到抑制;在1/2H 30%海水 10mmol·L-1CaCl2处理下,与1/2H处理相比菊芋生物产量、叶绿素含量和Pn显著增加,与1/2H 30%海水相比菊芋生物产量、叶绿素含量和Pn显著增加,MDA含量和相对电导率显著降低。由此证明外源Ca2 可有效缓解海水胁迫所致的氧化损伤,抑制脂质过氧化作用,增加叶绿素含量,维持较高的光合速率,促进干物质积累,从而使生物产量增加。

Abstract:RAPD was adopted to examine interspecific and intraspecific genetic diversities of 4 \%Emmenopterys\% populations with their twenty eight individual plants and 71 amplification sites were detected with 11 primers,which included 39 polymorphic sites.The an

Abstract:DNA is the carrier of genetic information and sequencing and comparison of DNA nucleotides are the thorough and ideal method for bryophyte molecular phylogenesis.The paper summarizes the applications of DNA sequences (cpDNA,nrDNA and mtDNA) in bryophyte p

Abstract:Polyamines are a group of physiologically active substances in plants and their metabolic changes have a close relation to flower bud formation and development in high plants.The paper summarized polyamine forms and distribution in plants and their roles

Abstract:The paper summarizes the applications of different molecular markers in the construction of genetic linkage maps,the locations of the genes related to important traits,the analysis of germplasm diversities,the identification of genetic relations,molecular

Abstract:Plant responses to abiotic stresses involve many genes and biochemical molecular mechanisms and stress-associated genes, proteins and metabolites form a complicated regulation network in which transcription control plays a balance-holding role.The paper m