Abstract:采用ELISA-DSM法和半叶枯斑法,测定了壳寡糖(50μg/mL)诱导后普通烟(Nicotiana tabacum)植株体内TMV浓度的变化.ELISA-DSM测定显示,在接种后10 d,仅在接种叶的上位叶和新生叶片中检测到病毒,且病毒浓度仅为不诱导对照的52.7%和38.8%,在下位叶中未检测到病毒;同时,接种叶内病毒增殖严重受抑,接种后10 d,病毒浓度仅为不诱导对照的23.52%.半叶枯斑法检测获得了相同结果,以壳寡糖处理植株的不同叶位的叶片为毒源,产生的枯斑数目都大幅度低于不诱导对照.以上结果证明,壳寡糖处理后TMV的上行和下行长距离移动均明显延迟和减少,下行移动受到的影响更大.透射电镜检查发现,处理植株接种叶的下位叶片韧皮部细胞中没有病毒晶体和病毒粒子,在上位叶片筛管伴胞中仅见少量病毒粒子,两者都未发现任何诱导新生物,也未见其他细胞结构变化.结果表明,壳寡糖处理使烟草对TMV病毒侵染产生了诱导抗病性,系统侵染症状明显减弱;壳寡糖处理对病毒长距离移动的不利影响可能是接种叶片病毒增殖减少所造成的.

Abstract:The Rubiscos(Ribulose-1,5-bisphosphate carboxylase/oxygenases) in different ecotypes of reeds growing in the desert region of Hexi Corridor were extracted and purified and their large and small subunit were separated by SDS-PAGE;and then Rubiscos holoenzy

Abstract:以辣椒(Capsicum annuumL.)幼苗为试材,研究了偏低温弱光(19℃/12℃昼/夜,90μmol?m-2?s-1)和临界低温弱光(15℃/8℃昼/夜,90μmol?m-2?s-1)胁迫10 d后的光合特性与光合作用启动时间的变化.结果表明:无论是在偏低温弱光还是在临界低温弱光下,辣椒幼苗的光补偿点(LCP)、光饱和点(LSP)、光饱和时的光合速率(Amax)和表观量子产额(AOY)下降;CO2补偿点(CCP)升高,而CO2饱和点(CSP)、CO2饱和时的光合速率(Amax)以及羧化效率(CE)下降;温度补偿点(TCP)降低;光合作用启动时间(STP)延长.在偏低温弱光下,辣椒幼苗有着更高的光与CO2利用能力和利用效率,光合作用启动时间较短,但温度补偿点较高.

Abstract:以小麦品种豫麦18为试验材料,研究了冠菌素和茉莉酸甲酯(MeJA)对小麦低温抗性的诱导作用.结果表明,冠菌素和MeJA处理可以显著提高低温胁迫下小麦幼苗细胞内SOD、POD和CAT等抗氧化酶的活性,增加可溶性蛋白的含量,降低相对电导率和MDA含量,从而维持细胞质膜的完整性,增强小麦植株抵抗低温胁迫的能力.冠菌素和MeJA在抗低温胁迫上具有相似的作用,1μmol/L的冠菌素和100μmol/L MeJA的诱导效果较好.

Abstract:One year old seedlings of Zizyphus jujuba with different drought resistances were treated with PEG-6000 at different concentrations to study the activities of antioxidative enzymes and the contents of osmosis-regulating substances in the seedlings of diff

Abstract:Nitraria sphaerocarpa seedlings were placed under different degrees of water stress simulated with PEG-6000 to investigate their variations in plant weights,and K~ ,Na~ ,MDA,free proline and soluble sugar contents.It was shown that under the water stress

Abstract:Two Cucumis melo L.varieties(C.melo var.inodorus and C.melo var.cantaloupsus) with thick fruit skin and one Cucumis melo L.variety(C.melo var.makuwa) with thin fruit skin,were used to study the effects of topping time and runner number on leaf-area enlarg

Abstract:As the explants,the leaf bases of germinating buds of garlic were induced to produce somatic embryos and SOD,POD and CAT activities and soluble sugar and protein contents in somatic embryogenesis were examined.It was shown that in somatic embryogenesis of

Abstract:以山茶属17个组130个物种为材料,通过相对电导率、丙二醛含量及超氧阴离子产生速率等生理指标测定及SPSS统计分析对其耐热性进行了研究.结果表明:持续1周36~38℃的高温胁迫能够有效区分不同山茶物种的耐热性;生理生化相关指标测定结果的系统聚类与主成分分析能够对不同山茶物种的耐热性进行有效评价.耐热性较强的有5个组,即离生雄蕊茶组、秃茶组、毛蕊茶组、茶组和连蕊茶组;耐热性最差为古茶组;部分组与组内物种的耐热性不完全一致,同组内不同物种的耐热性可能具较大差异.

Abstract:The total seed germination rates,seedling fresh weights and vigor indexes of four species,Elaeagnus angustifolia L.,Caragana korshinskii Kom.,Periploca sepium Bge and Fraxinus velutina Torr,were studied under droughty circumstances simulated with 1/2 Hoag

Abstract:The study investigated aboveground growths of Caragana korshinskii and Artemisia ordosica planted in four modes,revealing that in different planting modes their densities,heights and canopy width and biomasses remained unchanged or tended to decrease slig

Abstract:采用人工遮荫的方法,设置日光透过率100%、50.0%、25.0%、12.5%、6.3%、3.1%和1.6%7个光照强度处理,对日本矮生沿阶草(Ophiopogon japonicus)植株地上部和根系生长状况、叶片叶绿素含量、蒸腾速率和MDA含量等进行了观测和分析.结果表明,日本矮生沿阶草在25.0%~50.0%日照范围内(光照强度为176.3~352.8μmol?m-2?s-1)叶片叶绿素含量高、分蘖快、根系活力强、MDA含量低、地上部和根系生长良好,光照过低或过高均不利于其生长.

Abstract:采用不同浓度的人工合成甾类激素炔雌醇、炔诺酮和左炔诺孕酮处理拟南芥幼苗,研究3种激素对其根生长和根中内源激素含量的影响.结果表明:(1)30μg?L-1炔雌醇处理显著促进主根的伸长、侧根数及侧根总长的增加,促进主根根毛数和地下部鲜重的增加,增大内源IAA和iPAs含量,以及IAA/iPAs比值;3 000μg?L-1炔雌醇处理则增加主根的弯曲度;(2)炔诺酮活性相对较弱,浓度为300μg?L-1时对主根长、侧根数、侧根总长的促进效果最大,IAA含量增加而iPAs含量降低,IAA/iPAs比值最大;3 000μg?L-1炔诺酮则引起主根弯曲,抑制根毛的生长,但促进侧根数和的地下部鲜重的增加;(3)左炔诺孕酮的活性较高,3μg?L-1时对拟南芥主根长、主根根毛数增加最为显著;300μg?L-1时对侧根数、根毛密度的增幅最大,IAA含量增加,IAA/iPAs比值最大;3 000μg?L-1时显著抑制主根的生长和侧根的生成.

Abstract:There was male sterility Lycium barbarum germplasm YX-1 discovered in the production farm of 'Ningqi 1',a Lycium barbarum variety being dominatingly adopted in Ningxia,and its development and reproduction were further studied.It was shown that YX-1 plants

Abstract:Paraffin sectioning was employed to observe the microsporogenesis of male sterility pepper line 1A and its maintainer 1B and their morphological features at different microsporogenesis stages with light microscope.It was shown that in male sterility line

Abstract:研究了果梅(Prunus mumeSieb.1et Zucc.)花粉在不同培养基组分、花粉不同培养密度和不同温度及培养时间的离体萌发和花粉管生长特性.结果表明:细叶青花粉萌发及花粉管生长最适宜的液体培养基为30mmol/L MES(pH 6.5)缓冲液中含20%蔗糖,0.01%硼酸,20%PEG-4000,0.03?(NO3)2?4H2O,0.02%Mg-SO4?7H2O;萌发率达45.03%,花粉管长度达597.2μm.适宜于果梅花粉萌发和花粉管生长的花粉粒密度为20~80粒/μL.培养温度过高或过低都不利于果梅花粉的萌发和生长,25℃时花粉萌发和花粉管生长最好.细叶青"、月世界"、莺宿"3个品种的平均萌发率为48.6%,平均花粉管长度为762.3μm.果梅花粉在不同培养温度下,萌发及生长不同,在25℃条件下花粉管生长速度最快,集中在0~12 h内,3个品种花粉管平均生长速度为58.5μm/h.

Abstract:Video Cytological Image Analysis System and micrography were adopted to examine vessel elements in secondary xylem of Phyllanthus emblica.It was shown that vessel elements in secondary xylem of P.emblica have many different forms and most of them have tai

Abstract:Specific primers were designed according to the conserved sequences of orf224 and atp6 genes in GenBank and DNA sequences of orf224 and atp6 genes specific to pol cytoplasmic male sterility were obtained by amplifying genomic DNA of pol cytoplasmic male s

Abstract:以小麦特殊遗传材料———六倍体普通小麦阿勃二体、1A缺体、1B缺体和1D缺体,四倍体硬粒小麦墨西粒卡以及二倍体节节麦的总基因组DNA为模板,对D Ovidio等曾报道的硬粒小麦Glu-B3位点LMW-GS基因特异引物对P1(5-′tcctgagaagtgcatgacatg-3′)和P2(5-′gtaggcaccaactccggtgc-3′)进行了PCR扩增验证.结果表明,该引物对同样能特异扩增普通小麦Glu-B3位点LMW-GS基因.利用这对引物通过AS-PCR方法克隆得到优良小麦品种小偃6号1B染色体1个LMW-GS基因片段.该基因全长为1 089 bp,包含了完整的编码区和其上游318 bp的胚乳特异表达启动子区.该基因被命名为XY-Glu-B3-LMW2(GenBank登录号为DQ630442).XY-Glu-B3-LMW2的推测蛋白含256个氨基酸(包括N-端20个氨基酸的信号肽),其成熟蛋白有8个保守的Cys残基,均分布在C-末端区.XY-Glu-B3-LMW2是从小偃6号克隆到的第2个LMW-GS基因.

Abstract:以中国李叶片基因组为模板,PGIP基因保守序列为引物,扩增到1条全长1 192 bp的目的片段(Genbank登录号:DQ200847).序列分析表明:该片段与杏、桃、马哈利樱桃、梅的PGIP基因序列一致度达96%~99%,包含有1个完整的开放阅读框和1个内含子,其编码的氨基酸序列中含有一段典型的亮氨酸重复序列;分子进化分析表明,在分子进化树中,该序列与杏、桃、马哈利樱桃、梅的PGIP序列位于同一类.为植物分子抗病育种提供了1条基因资源.

Abstract:Oligo-capping法是构建全长cDNA文库的重要方法之一.以名贵中药手掌参(Gymnadenia conopseaR.Br.)的幼芽组织为材料,通过减少mRNA的用量,用pUC18作载体,设计特异引物对第1链cDNA进行PCR扩增,按片段大小分级回收cDNA,形成了一种以Oligo-capping法为基础,构建珍稀材料全长cDNA文库的快速、简单的技术体系.利用该方法,首次成功地构建了手掌参的全长cDNA文库.经综合评价,文库容量达到了8×105个/μg cDNA,全长cDNA比例达到68%,重组率超过96%,说明本实验的改进非常成功.该文库的建成为手掌参的遗传资源保护和功能基因发掘等理论与应用研究奠定了基础.

Abstract:Wheat was transformed with soybean DNA through particle beam mediation and its high-protein offspring plants were obtained through field selections and protein determinations in the four consecutive generations.34 random primers was employed to amplify ge

Abstract:用ISSR标记技术对10个大豆品种进行遗传多样性分析.从44条随机引物中筛选出8个多态性引物,共扩增出89条带,其中有55条为多态性条带,多态性比率为61.8%,条带大小为220~1 500 bp,平均每个引物可扩增出11条带.Shannon多样性指数评价结果表明,平均多样性指数为0.286 5,观察等位基因数和有效等位基因数分别为1.618和1.300 8;统计分析结果表明,10个品种间的相似系数为0.60~0.75,平均相似系数为0.69;聚类分析结果表明,10个大豆品种可聚成2组:第一组包括豫豆15、豫豆11、豫豆24、周豆12和周豆11;第二组包括豫豆22、周豆13、豫豆6、中作98-3和豫豆26;主成分分析结果支持聚类分析结果.本研究为大豆品种鉴定和种质资源利用奠定了基础.

Abstract:对加拿大披碱草×披碱草F1和加拿大披碱草×圆柱披碱草F1的幼穗外植体愈伤组织诱导及植株的再生进行了研究.试验筛选出2个杂交种愈伤组织诱导的最适培养基均为:MS 400 mg/L CH 3.0 mg/L 2,4-D;最适继代培养基为MS 400 mg/L CH 1.0 mg/L 6-BA 0.5 mg/L 2,4-D;最适幼苗分化培养基为:MS 400 mg/LCH 3.0 mg/L 6-BA 0.5 mg/L NAA;最适生根培养基为:MS 0.5 mg/L NAA 0.5 mg/L IBA.这一组培体系的建立,为2个种间杂交种的种质保存及利用奠定了基础.

Abstract:选用9个辣椒(Capsicum annuumL.)品种(系),研究了不同激素组合、基因型、外植体类型、苗龄和Ag-NO3等因素对外植体不定芽分化和伸长的影响.结果表明,在6-BA/IAA为10∶1配比下,有利于辣椒外植体的分化再生,而6-BA/IAA为3∶1配比下适合于再生芽的伸长;不同品种辣椒的再生能力差别较大,分化率在13.3%~90.0%之间;辣椒子叶再生能力比下胚轴强,是较好的外植体材料;12~16 d苗龄的外植体分化频率较高;添加4mg?L-1AgNO3可使芽分化率平均提高16.9%.通过比较,筛选出了适合于辣椒芽分化的培养基为MB5(MS无机盐 B5有机成分) 5 mg?L-16-BA 0.5 mg?L-1IAA 4 mg?L-1AgNO3,芽伸长培养基为MB5 3 mg?L-16-BA 1 mg?L-1IAA 2 mg?L-1GA3 4 mg?L-1AgNO3,生根培养基为1/2 MS 0.2 mg?L-1IAA 0.1 mg?L-1NAA.

Abstract:Two year old Cunninghamia lanceolata seedlings,growing in Shangnan County,were inoculated with three ecotomycorrhizal fungi,Suillus bovines,Suillus luteus and Boletus luridus.It was shown that the ectomycorrhizal fungi remarkably raised the survival rate,

Abstract:Andrographolide was acetylated with acetic anhydride to form acetyl andrographolide and then the anti-microbial activities of acetyl andrographolide and andrographolide to some bacteria,mildews and yeasts and phyto-pathogens and their allelopathic effects

Abstract:Eucommia ulmoides Oliv.was planted in the forms of foliage and arbor forests and secondary metabolite contents in its bark in the planting modes were determined.It was shown that secondary metabolite contents in different planting modes differed.Eucommiol

Abstract:运用静态吸附试验对8种大孔树脂进行筛选,通过单因素试验、正交试验和方差分析确定了菊苣菊粉脱色的最佳操作条件.结果表明,LSD-263树脂对菊苣菊粉色素的吸附性能较好;最佳脱色条件为:上柱温度45℃,色素浓度(以吸光度计)0.773 Abs,上柱速度3 BV/h.在此条件下,LSD-263树脂对色素的吸附量可达14.48△AV/mL,菊粉损失率仅为2.38%.

Abstract:采用路线调查法和样方法研究了陇东黄土高原丘陵沟壑区天然草地植物群落组成的特征.结果表明,该研究区地带性植被的主要类型为草甸草原和典型草原,草甸草原以蒿类、白羊草和白草为代表群落;典型草原以长芒草、蒿类、百里香和毛沙芦草等为代表群落.草地群落物种组成较为简单,优势种的优势度显著,其中长芒草、山蒿、冷蒿、牛枝子、糙隐子草、铁杆蒿、阿尔泰狗娃花、赖草、猪毛蒿、百里香等是群落物种组成中相对重要的物种;天然草地群落数量特征的平均值为:种数17.1、多度163.5、高度49.2 cm、盖度52.8%、鲜重284.9 g?m-2、干重151.8g?m-2;草甸草原群落的高度、盖度、生物量和物种丰富度值均大于典型草原.

Abstract:于1990~2005年对大巴山进行了野外考察,并对所采集的蕨类植物标本进行鉴定及资料统计分析.结果表明,大巴山具有蕨类植物34科76属247种(包含变种和变型).鳞毛蕨科、水龙骨科和蹄盖蕨科为大巴山蕨类植物区系中占优势的3大科,共有113种,占总种数的45.75%.属的地理成分以热带、亚热带类型为主,约占总属数的65.62%,温带类型占34.38%.在种的水平上,热带、亚热带成分仅占总种数的10.29%,温带成分处于优势地位,为89.71%.在温带成分中,东亚成分与中国特有成分显著,共占总种数的84.36%,表明大巴山应属于中国-日本蕨类植物亚区.

Abstract:Thirteen species newly recorded fern species in Guangzhou are presented,which belong to 11 genera and 10 families.In which Hypolepidaceae is the newly recorded family from Guangzhou.

Abstract:Seven species are reported as new records to Shaanxi Province,viz.Smilax vanchingshanensis(Wang et Tang) Wang et Tang(Liliaceae),Berberis zanlanscianensis Pamp.(Berberidaceae),Cardamine violifolia O.E.Schulz(Cruciferae),Camelina microcarpa Andrz.(Crucifer

Abstract:对1年生库拉索芦荟分别用盐(1.8%的NaCl)、低温(10℃)、干旱[25%(w/v)的聚乙二醇-6000]3种胁迫条件处理7d后,对其叶肉细胞超微结构进行观察.结果发现:3种胁迫处理均可使库拉索芦荟细胞膜系统、叶绿体、线粒体、细胞核等结构受到不同程度的破坏,叶绿体周围出现许多小泡,导致细胞内膜系统紊乱,细胞器结构稳定性降低;盐胁迫下高尔基体在细胞质中解体;盐和低温胁迫下均可见线粒体膜与叶绿体膜发生融合、线粒体嵌在叶绿体当中的现象.另外,本研究发现,盐胁迫、低温胁迫比干旱胁迫对库拉索芦荟细胞膜的损伤更严重,而水分胁迫对其的伤害程度较小,表明库拉索芦荟的抗旱性较其抗盐性更强.

Abstract:利用高效液相色谱（HPLC）法对宁夏中部干旱带半野生乌拉尔甘草营养器官中甘草酸含量的动态变化规律进行了研究.结果表明：地下营养器官（根和根状茎）是甘草酸主要积累部位,而地上茎和叶中甘草酸含量很低甚至检测不到.就地下器官而言,甘草酸的积累随生长年限增加而递增,且1～4 a生甘草均表现出主根中甘草酸的含量高于根状茎的规律.分别对3 a、4 a生的半野生甘草根和根状茎进一步研究发现,在一个生长季节中甘草酸含量的变化呈现相似的变化规律.即从7～10月份,甘草根和根状茎中甘草酸含量呈波动性上升趋势,10月份达到最大值.

Abstract:以秦美猕猴桃为材料,对不同施肥处理的果实后熟过程中软化、腐化过程进行观察统计,并在果实采后4个具有代表性的后熟时期(硬度分别为15 kg/cm2左右、8~7 kg/cm26、~5 kg/cm25、~4 kg/cm2)进行可溶性固形物、可滴定酸、Vc、可溶性糖等营养指标测定.结果显示:(1)从后熟过程中软化、腐化统计看,3个施肥处理(A、B、C)的贮藏品质与对照(CK)间差异明显,C处理(氮肥 磷肥 农家肥)的猕猴桃贮藏期最长,软化、腐化出现得最晚,较对照分别延长15 d、27 d,贮藏性最好;(2)3个施肥处理的营养指标与CK间差异明显,C处理与CK和A处理差异显著.表明在猕猴桃生产中采用氮肥 磷肥 农家肥相结合的施肥方式可显著延长猕猴桃果实贮藏期(后熟期),并可显著提高猕猴桃果实的食用和营养品质.

Abstract:Fat-soluble constituents of hemp kernels were extracted by Soxhlet extraction and then identified and determined by GC/MS.There were 29 fat soluble constituents identified of which fatty acid methyl esters made up 99.32%(12.36% saturated fatty acid methyl

Abstract:本文对菊科16个属———刺苞果属(Acanthospermum)、蓍属(Achillea)、胜红蓟属(Ageratum)、蒿属(Arte-misia)、紫苑属(Aster)、Balsamorhiza、雏菊属(Bellis)、鬼针草属(Bidens)、艾纳香属(Blumea)、天明精属(Carpe-sium)、红花属(Carthamus)、矢车菊属(Centaurea)、茼蒿属(Chrysanthemum)、菊苣属(Cichorium)、秋英属(Cos-mos)、大丽花属(Dahlia)中具有杀菌活性的植物按属的顺序进行了综述.表明在16个属中主要杀菌植物集中在蓍属(Achillea)、胜红蓟属(Ageratum)、蒿属(Artemisia)、天明精属(Carpesium)和矢车菊属(Centaurea)等5个属中,为进一步开展菊科植物资源的植物性杀菌剂研究和开发利用提供参考.

Abstract:In plants,somatic embryogenesis can be employed as not only an important propagation measures but also an important model system for embryo development research.Morphological and physiological maturities of somatic cells directly affect plantlet germinati