Abstract:One important resource,genomic sequence of Indica '93-11'(the pollen provider of super-hybrid-rice),has been ready for the research of crop genetic improvement and comparative genomics among crops.But,there are so many gaps in it.In order to upgrade the p

Abstract:One important resource,genomic sequence of Indica '93-11'(the pollen provider of super-hybrid-rice),has been ready for the research of crop genetic improvement and comparative genomics among crops.But,there are so many gaps in it.In order to upgrade the p

Abstract:Using leaves of Brassica napus cv.'Huyou No.15' as material,RACE-PCR was employed to clone a new cDNA encoding a protein with a calculated molecular weight of about 14 kD from B.napus(BnCOR14,AY456378).The full-length cDNA of BnCOR14 was 564 bp and contai

Abstract:Based on the reported amino acid sequence of buckwheat trypsin inhibitor(BTI) and the partial gene sequence obtained by our lab,special primers were designed,buckwheat trypsin inhibitor gene was amplified through RT-PCR.The BTI gene was cloned into the ex

Abstract:以大麦、小麦、玉米、高粱和苏丹草的种子为材料提取种子的醇溶蛋白,分析了不同提取剂及不同固液比[种子粉末样品质量(g)与提取剂的体积(mL)的比例]对种子醇溶蛋白提取效果的影响,并对SDS-PAGE检测醇溶蛋白中的不同胶浓度、厚度以及样品上样量等的影响进行了研究.结果表明,60%的正丙醇、乙二醇、异丙醇和叔丁醇分别是小麦、大麦、玉米以及高粱和苏丹草的最佳提取剂,将1∶6比例提取的种子醇溶蛋白以15μL上样,0.5mm厚度的15%分离胶电泳可以得到清晰的电泳检测图谱.

Abstract:Tetraploid plants of non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino) were induced by treating the apical portion of seedlings with different solution of colchicines.Autotetra ploid plants were selected and identified based on morphol

Abstract:Nineteen varieties of radish were used in the experiment to study the effects of different factors on isolated microspore culture of different genotypes of radish.It found that(1) different frequencies of embryos were obtained among 13 genotypes,the induc

Abstract:以芸芥子叶为外植体,诱导芸芥体细胞胚胎发生并建立植株再生体系.结果表明:基因型及植物生长调节剂对芸芥体细胞胚胎发生均有一定的影响,其中以含有1.0mg·L-12,4-D的MS培养基诱导芸芥体细胞胚胎发生的效果最优.在MS 0.2mg·L-12,4-D培养基上,胚性愈伤组织可大量增殖.对芸芥体细胞胚胎成熟的研究表明,体胚在N6培养基上成熟最佳,且45.2%的成熟体胚可在1/2MS 0.1mg·L-1IBA培养基上萌发生长.

Abstract:Based on the field investigations and laboratory examinations,the distribution patterns of sporophytes in the dioecious epiphytic moss Dolichomitriopsis diversiformis(Mitt.) Nog.in Fanjing Mountain of Guizhou in China were studied,the results were describ

Abstract:Salicylic acid is an endogenous signal with general functions in plants. The effect of salicylic acid on stomatal movement in guard ceils of Vicia faba was studied by laser scanning confocal microscopy and the results showed that salicylic acid could indu

Abstract:The impact of dehydration stress on the apoplasts and the microenvironment of cells in leaves in Forsythia suspensa(Thunb.) Vahl and Euonymus japonicus Thunb.were analysed by means of the pressure probe,electrolyte conductivity,and atomic absorption spect

Abstract:分别采用5mW·mm-2He-Ne激光辐照和增强UV-B辐射(10.08kJ·m-2·d-1)及其二者组合对晋麦8号小麦幼苗进行处理,5d后测定各处理幼苗叶片可溶性蛋白、硝酸还原酶和谷氨酸脱氢酶活性的变化以分析He-Ne激光对小麦幼苗受增强UV-B损伤的修复作用.结果显示:He-Ne激光辐照可使UV-B辐射后小麦幼苗叶片的可溶性蛋白含量增加,谷氨酸脱氢酶活性增强,而使硝酸还原酶活性先升高后降低.表明小麦幼苗叶片可溶性蛋白、谷氨酸脱氢酶、硝酸还原酶的变化同小麦幼苗损伤修复的能力相关,一定剂量的He-Ne激光辐照可部分修复增强UV-B对小麦幼苗氮代谢水平的辐射损伤.

Abstract:用禾谷镰刀菌粗毒素对赤霉病抗性不同的6个小麦品种(系)幼苗进行处理,在1h~10d内测定各品种(系)的MDA含量、SOD及PAL活性增率的变化.结果显示:禾谷镰刀菌粗毒素处理后,抗病和中抗品种(系)幼苗的MDA含量增率低于感病品种(系),且增加速度慢;抗病和中抗品种(系)幼苗的SOD活性在胁迫1h后迅速升高,其活性增率高于感病品种(系);抗、感品种(系)PAL活性均升高,但抗病品种(系)PAL活性增加快,且活性增率高于感病品种(系).研究表明,小麦幼苗的SOD、PAL活性增率与品种抗病性呈正相关,MDA含量增率与品种抗病性呈负相关.

Abstract:The effect of an arbuscular mycorrhizal(AM) fungus,Glomus versiforme,on nutrient uptake in leaves of trifoliate orange [Poncirus trifoliata(L.) Raf.] seedlings was investigated in pot experiments under well-watered and water stress conditions.Mycorrhizal

Abstract:以天然盐生植物碱地肤(Kochia sieversiana)为材料,采用人工生态模拟方法对其施加0~480mmol/L的盐胁迫(摩尔比1∶1的NaCl和Na2SO4)和碱胁迫(摩尔比1∶1的NaHCO3和Na2CO3),测定了盐碱胁迫下的相对生长率(RGR)、叶绿素含量及丙二醛含量.结果表明:低浓度的盐胁迫(80mmol/L)对碱地肤的生长具有刺激作用,其在高达480mmol/L的盐胁迫或400mmol/L的碱胁迫下仍能存活并维持一定的生长,具有强抗盐性和强抗碱性;在相同盐浓度下,碱地肤受碱胁迫的伤害强度和对碱胁迫所做出的胁变反应均大于对盐胁迫.可见,碱地肤的抗盐性强于抗碱性,受碱胁迫的伤害大于盐胁迫,维持高含水量可能是碱地肤适应高强度盐碱胁迫的重要特性.

Abstract:以强筋面包小麦临优145为试验材料,于大田生产条件下研究了施用钾肥对强筋小麦叶片光合、膜脂氧化及产量特性的影响.结果显示,适量施钾能显著降低开花14d以后旗叶中超氧阴离子的产生强度和MDA含量,并显著提高旗叶的叶绿素含量、光合速率和籽粒灌浆速率,显著增加千粒重;在施钾(K2O)37.5~150.0kg/hm2范围内,随施钾量增加,各处理产量呈先上升后下降趋势且均比对照极显著增产14.5%~30.7%,各处理间产量差异显著,以施钾(K2O)112.5kg/hm2时籽粒产量最高.结果表明,中产条件下施用钾肥可显著提高强筋小麦籽粒产量,且以施钾(K2O)103.0~112.5kg/hm2为宜.

Abstract:The growth and water metabolism of three tree species on the Loess Plateau was studied under soils with different water contents.The results showed that water consumption of those species decreased with the increase of drought stress,and water consumption

Abstract:采用PV技术对8个杨树无性系(3、6、7、8、9、10、11、12号)的水分参数ψstlp、ψssat、RWCtlp、ROWCtlp、AWC、ξmax和b值等进行了研究,并采用抗旱性指数(DI)和模糊数学反隶属函数两种方法对各个无性系的抗旱性进行了综合评价.结果显示:8个无性系的ψstlp、ψssat、ROWCtlp、ξmax、b值不同,抗旱性也存在一定的差异.其中,11、7和8号维持膨压和渗透调节的能力比其余无性系强;11号具有很强的保持最大膨压和维持低水势的能力;7~9号无性系具有较强的忍耐水分胁迫和抗脱水的能力;3和6号细胞弹性最大,主要依靠其坚硬厚实的细胞保持体内的水分.结果表明,8个无性系分为3个类群:第一类群为无性系7、8、9、11、12号,抗旱性最强;第二类群为无性系10号,抗旱性居中;第三类群为无性系3号和6号,抗旱性最弱.

Abstract:The effects of different photoperiods on in vitro plantlet regenerations from leaves of Paulownia tomentosa,P.fortunei and P.elongata on the optimal MS and 1/2 MS media for their in vitro organogenesis respectively were studied.The results indicated that

Abstract:The Plant community from out-door pot experiment was constructed to study the relationship of the diversity,productivity and invisibility,as well as the possible mechanisms with 8 annual species.The results indicated that monocultures varied greatly in th

Abstract:The symbiosis of plants with VA mycorrhizal fungi in saline-alkali soil of Gansu was studied and this showed that the VA mycorrhizal fungi colonized all 17 species of 10 families but Suaeda glauca Beg. and the colonization rate was 94. 1% ;there was high

Abstract:新疆乌恰县具有独特的地理环境与气候条件,蕴藏着丰富的结瘤豆科植物资源.本研究从乌恰县豆科植物根瘤中分离到40株根瘤菌,并进行唯一碳源利用、唯一氮源利用、对抗生素和染料的抗性、耐盐性、初始pH生长、生长温度范围及石蕊牛奶反应、氧化酶、过氧化氢酶、脲酶共118项生理生化性状测定.结果表明:乌恰县豆科植物根瘤菌在碳氮源利用、抗生素敏感性、对染料抗性程度等方面存在着差异.供试根瘤菌能耐受较低温度,并具有较强的耐盐、碱能力,均能在pH 6~12的YMA培养基上生长,有60%的菌株能耐受0.86mol/L的NaCl.数值分类结果表明,在84.2%的相似水平上40株供试菌株构成了4个新的表观群.第Ⅰ、Ⅱ、Ⅲ和Ⅳ类群分别有8、9、3和3株菌,中心菌株分别为NWWQ73-2、NWWQ13-1、NWWQ1-2、NWWQ6-2.此外,乌恰县豆科植物根瘤菌与模式菌株的相似性较低,它们所形成的4个独立群可能有新种存在.

Abstract:Research was conducted to investigate sod culture and sod laying of three kinds of turfgrasses by using municipal solid waste compost as medium and 5 waste materials(fibrous woven sack,plastic window gauze,plastic film,bamboo mat and straw mat) as netting

Abstract:在黄龙山林区以4种不同白桦林群落类型为研究对象,共设置20 m×20 m的样地15块,从林分特征、物种组成、物种多样性、灌木和草本生物量及乔木蓄积量等方面,对不同白桦林群落类型结构特征进行调查分析.结果显示：（1）群落B林（白桦＋茶条槭昆交）中白桦林分状况为最好,其次为群落A（油松＋白桦混交林）,群落D（自桦纯林）最差;（2）不同群落中植物种类分别为群落A（51种）＞群落B（49种）＞群落D（48种）＞群落C（38种）;（3）物种多样性指数为群落A＞群落B＞群落C＞群落D;（4）不同群落内草本和灌木总生物量排序为群落D（73.19t·hm^-2）＞群落B（65.07 t·hm^-2）＞群落C（59.93 t·hm^-2）＞群落A（44.82 t·hm^-2）,乔木蓄积量为群落A（123.50 m^3·hm^-2）＞群落D（69.50 m^3·hm^-2）＞群落B（53.25 m^3·hm^-2）＞群落C（50.25 m^3·hm^-2）.研究结果表明,黄龙山不同白桦林群落中混交林在生长状况、物种组成、物种多样性和生物量方面均优于纯白桦林.表明白桦混交林对保持黄龙山森林群落稳定性,提高林分质量,实现白桦林向松栎林的逐渐过渡有重要意义.

Abstract:马峦山地区位于广东深圳东北部,地理位置为北纬22°37′~22°39′,东经114°17′~114°22′,周围林地面积达上百平方千米.本研究对马峦山地区种子植物区系131科482属907种进行分析,结果表明:(1)表征科主要为樟科、芸香科、鼠刺科、山茶科、含羞草科、桃金娘科、五加科、桑科、藤黄科和五列木科、壳斗科、省沽油科、山矾科、安息香科、金缕梅科、杜鹃花科、买麻藤科等.(2)属的地理成分多样,以热带亚热带成分占绝对优势,热带分布、热带至亚热带分布的科、属分别占总科数的85.72%、总属数的86.67%.(3)区域特有现象明显:中国特有属5属,含6种;亚洲热带-亚热带特有属11属,含16种;东亚特有属14属.(4)马峦山种子植物区系与邻近的尖峰岭、内伶仃岛、南岭、香港、黑石顶区系比较,科的相似性指数分别是95.19%、70.19%、89.42%、95.19%和92.31%;属的相似性指数分别为87.55%、57.17%、69.20%、94.13%和72.78%.

Abstract:采用GC-MS联用技术检测了阴干的大花金挖耳花蕾中精油的化学组成及相对含量,并测定了该精油对14种常见作物病原菌的抗菌活性.结果表明,大花金挖耳花蕾精油得率为2.9%,从中鉴定出53种成分,占色谱峰总面积的86.01%,主要化学成分为（E,E）-3,7,11,15-四甲基-1,6,10,14-十六烷四烯（15.33%）、甲苯（11.32%）、（3α,5α）-3,5,14,19-四羟基强心甾-20（22）-烯（6.00%）、2-乙氧基四氢呋喃（5.44%）、亚油酸（3.51%）等.杀菌实验结果表明,该精油对小麦全蚀病菌（Gaeumannomyces gramims）和小麦纹枯病菌（Rhizoctonia cerealis）抑制作用最强,在0.5 mg/mL浓度下,5 d后对这2种病菌菌丝生长的抑制率均大于97%;对小麦赤霉病菌（Fusarium graminearum）和番茄叶霉病菌（Fulvia fulva）等也有较强的抑制效果.大花金挖耳花蕾精油的化学组成及其杀菌活性研究均为首次报道.

Abstract:为研究砂地柏杀虫化学成分特征,采用活性示踪法,从砂地柏果实乙醇提取物中分离鉴定出6种萜烯类化合物:4-表-松香醛(1)、7,13-松香二烯-3-酮(2)、4-表-松香醇(3)、日本扁柏酮(4)、β-谷甾醇(5)和豆甾醇(6),其中日本扁柏酮是从砂地柏中首次分离得到.以砂地柏中另2种主要杀虫活性成分鬼臼毒素和脱氧鬼臼毒素为对照品,采用小叶碟添加法和浸叶法分别测试了上述化合物对粘虫(Mythimna separata)幼虫的拒食活性和对菜青虫(Pieris rapae)的毒杀活性.结果表明,2种木脂素类化合物鬼臼毒素和脱氧鬼臼毒素的活性高于本研究得到的6种化合物,其中4种二萜类化合物的活性高于2种甾醇三萜类化合物;4种二萜类化合物的活性差异较大,其中4-表-松香醇和7,13-松香二烯-3-酮对粘虫幼虫的拒食活性较高,其48 h AFC50分别为2.92和3.02mg·mL-1,7,13-松香二烯-3-酮对菜青虫毒杀活性较高,其48h LC50为3.40mg·mL-1.所得到的4-表-松香醇和日本扁柏酮2种化合物的杀虫活性为首次报道.

Abstract:描述了产于中国新疆的黄耆属（Astragalus）丁字毛类群2新种,即额尔齐斯黄耆（Astragalus eerqisiensis Z.Y.Chang,L. R. Xu ＆ D.Podlech）和沙地黄耆（A.shadiensis L. R. Xu,Z.Y.Chang ＆ D.Podlech）.其中,额尔齐斯黄耆仅见于新疆布尔津县的额尔齐斯河流域,与哈巴河黄耆（A.habaheensis）近缘,区别在于前者小叶菱形、倒卵形或椭圆形,长15～25（30）mm,宽（3）7～12（15）mm,旗瓣长20～22（25）mm,翼瓣顶端2裂;沙地黄耆产于新疆托克逊县东北部,散生于沙地荒漠,形态上与变异黄耆（A. variabilis Bunge ex Maxim.）接近,区别在于小叶5～7（9）枚而非11～19枚,旗瓣在中部收缩,植株全体被灰色毛而呈灰色.2新种均系中国特有种,其中额尔齐斯黄耆隶属于乌拉尔组（A. Sect.Helmia Bunge）,沙地黄耆隶属于旱生组[A. Sect.Craccina（Stev.）Bunge].

Abstract:A new form(S.oppositifolia Linn.form.albiflora Y.H.Wu)of Saxifraga oppositifolia Linn.is described from Xinjiang,China.The type was collected from Nuryamei,Qira County by Qinghai-Xizang Comprehensive Scientific Exploring Party during their exploring on th

Abstract:Tibetia himalaica(Baker) Tsui and Oxytropis xinglongshanica C.W.Chang are reported as new records to Ningxia.

Abstract:Lectotypes are here designated for Betula jiulungensis Hu ex P.C.Li and Carpinus tientaiensis W.C.Cheng(Betulaceae),because two specimens were simultaneously designated as types,but no holotype was indicated in the protologue.

Abstract:The present paper supplemented the flora of Guizhou Province.of these,fourteen species,two varieties and two genera are new records to Guizhou Province,namely:Clematis gratopsis W.T.Wang,Hepatica henryi(Oliv.) Steward,Thalictrum reticulatum Franch.,Yinsha

Abstract:以‘秦光2号＇油桃为材料,研究了5个采收成熟度油桃果实在冷藏过程中呼吸速率、硬度、可溶性固形物含量（SSC）、可滴定酸含量（TA）的变化.结果表明：（1）冷藏过程中,完熟果实（第V成熟度）在贮藏15 d后最先到达呼吸高峰,未成熟果实（第Ⅰ、Ⅱ、Ⅲ、Ⅳ成熟度）则成熟度越低呼吸高峰出现的越早,且峰值越高.（2）冷藏过程中,果实硬度和TA逐渐下降,不同成熟度果实硬度变化除第Ⅲ成熟度和第Ⅳ成熟度之间差异不显著外,其余成熟度间均有显著差异（P＜0.05）;不同成熟度果实在贮藏前期TA差异显著,但后期差异不显著;SSC在贮藏过程中表现出先升高后下降趋势.（3）在冷藏条件下,成熟度越低的果实对低温的敏感性越高,越易发生冷害.（4）第Ⅳ成熟度果实（果实底色呈乳白色,SSC 11.4%,硬度7.3 kg·cm^-2）在冷藏期间呼吸高峰出现时间晚,能保持相对较高的果实硬度和可溶性固形物含量,贮藏后货架期好果率高,贮藏效果佳,为最适宜的采收成熟度.

Abstract:采用2个BAU-9403剂量,4种处理方式对4个基因型小麦的主茎叶片进行处理,通过检测各处理分蘖穗的雄性不育性及其叶片的光合特性,探讨BAU-9403的吸收和转运效果.结果表明:(1)小麦主茎叶涂施BAU-9403后,不仅诱导主茎穗雄性不育,且可吸收转运到分蘖穗产生雄性不育;(2)分蘖叶叶色变淡,叶绿素含量较CK低,较主茎叶高,差异均显著;(3)主茎叶和分蘖叶的净光合速率分别为7.89和8.87μmol CO2·m-2·s-1,均比CK(10.45μmol CO2·m-2·s-1)低,主茎叶与CK差异显著(T=2.447);(4)分蘖穗不育度因基因型敏感性、处理方式和剂量的不同而异.说明BAU-9403处理小麦后可吸收转运到未处理部位产生效果.对反应敏感的基因型,使用较大剂量和增大处理面积利于BAU-9403的吸收转运,但对植株会有一定程度的损伤,对光合性能产生不利影响.

Abstract:以转hGM-CSF基因鱼腥藻7120为对象,分别探讨了温度、pH、光强等环境因子和外加碳、氮等基本营养源对转基因藻生长与光合活性的影响.结果表明:当基本环境因子为30℃、pH9.5和90μmol·m-2·s-1光强时,转基因藻生长最快.添加10mmol·L-1的NaHCO3或5g·L-1的葡萄糖对转基因藻的光合活性促进最大;但外加氮源却抑制了转基因藻的光合活性.

Abstract:The content of lignin and cellulose of Neosinocalamus affinis and Bambusa rigida with the typical thick-growing characteristics was studied with samples from different regions in Sichuan province.The results showed that the cellulose content was different

Abstract:Sunflower(Helianthus annuus L.)is one of the major oil crops in the world.In recent years,mone research works have been done on sunflower,among which the study on tissue culture of sunflower has been paid more and more attention and some progress has been

Abstract:Plant somatic hybridization provides the possibility of genetic material recombination of sexual cross-incompatibility plants,results in novel plant cultivars and even new species.It is a preponderant technique,especially for improving agronomic traits co