Abstract:CMO(choline monooxygenase)and BADH(betaine aldehyde dehydrogenase),the two genes encoding the key enzymes of betaine synthesis in Suaeda salsa,were cloned by PCR according to the homologous sequence of which in Suaeda liaotungensis.The nucleotide sequence

Abstract:以草莓栽培品种哈尼"为材料,利用根癌农杆菌介导的叶盘法将拟南芥冷诱导转录激活因子CBF1(C-re-peat binding factor)导入草莓中.转化植株经25 mg/L Km筛选后进行PCR和Southern杂交鉴定,结果显示CBF1基因整合进草莓基因组中.采用电解质渗漏法和生长恢复法对转基因株系进行抗寒生理鉴定,结果显示,转基因株系的电导率普遍低于对照;将转基因株系和对照于-2-0℃放置7 d,在转基因株系83中有65%的植株出现了萎蔫,而对照植株有91%出现了萎蔫,经22-25℃下进行恢复生长,转基因株系83有74%完全恢复,而对照株系只有18%恢复.结果表明CBF1基因导入草莓中可以提高草莓对低温胁迫的抵抗力.

Abstract:An Arabidopsis mutant(line 155) induced by T-DNA insertion was studied carefully in phenotypes,microstructures of shoot apical meristem(SAM) and histo-chemistrical localization of GUS gene through comparison with wild type.The results showed that(1) the m

Abstract:We used cDNA-AFLP technique to analyze gene expression profiles during somatic embryogenesis of hypocotyls section(explant),callus after forty days induction on medium of MS and somatic embryo in cotton(Gossypium hirsutum L.) TM-1.cDNA-AFLP was analyzed i

Abstract:以南丰蜜橘[Citrus reticulata Blanco var.kinokuni(Tanaka)H.H.Hu]基因组DNA为模板,根据已报道的柑橘β-胡萝卜素羟化酶基因保守序列设计4对引物,进行PCR扩增,得到4条长度为470、761、294和991 bp的片段.将这些片段克隆到pMD18-T载体,并进行测序.测序结果拼接成1条2 326 bp的序列.分析发现该序列含6个内含子,7个外显子.内含子两侧有典型的GT-AG保守序列.该序列中预测的编码序列与温州蜜柑、拟南芥等植物的β-胡萝卜素羟化酶基因序列保守性达80%以上,表明该序列确实为β-胡萝卜素羟化酶基因.该基因编码了1个含311个氨基酸的蛋白.将该序列递交到GenBank数据库,序列号为AM408552.

Abstract:Genetic diversity and relationship of 48 melon(Cucumis melo L.)germplasms were studied by molecular marker technology AFLPs(Amplified fragment length polymorphisms).Ten selective primer pairs were screened from total 64 primer pairs and 172 polymorphic ba

Abstract:采用过氧化物酶同工酶(POD)和酯酶同工酶(EST)对菊属8个种植物的27份材料(不同地理居群或品种)的遗传多样性进行了分析.结果表明:(1)27份材料共获得12个同工酶位点,以及13种POD条带和15种EST条带;(2)27份材料用UPGMA聚类为两组,第Ⅰ组为野生菊、药用菊,第Ⅱ组为观赏菊,前者品种较为原始,后者品种较为进化;(3)野菊12个不同居群的平均观察杂合度(Ho)为0.166 7-0.750 0,其中南京野菊遗传变异最大,河南野菊最小;(4)菊属8个种的遗传一致度为0.461 6-0.860 5,平均遗传距离为0.451 7,其中栽培菊与野菊的亲缘关系最近(遗传一致度为0.860 5),野菊可能是栽培菊的一个主要起源种.

Abstract:以黄花补血草(Limonium aureum(L.)Hill.)无菌苗为材料,研究了不同激素配比条件下不同外植体愈伤组织的诱导及植株再生.结果表明,黄花补血草无菌苗叶片、叶柄和幼根均可作为离体培养的外植体,但叶片和叶柄的诱导率明显高于幼根.将外植体接种于分别添加0.5 mg/L NAA或1.0 mg/L 2,4-D或0.3-0.5 mg/L 6-BA 0.5-2.0 mg/L NAA的MS培养基上,经过14-28 d培养后,可脱分化产生乳白色、红色或浅绿色颗粒状或致密愈伤组织,频率达到70%以上.在MS 0.3 mg/L 6-BA 1.0 mg/L NAA培养基上,红色和绿色颗粒状愈伤组织经过1-2次继代后,均可分化产生不定芽,进而形成丛生芽,分化率达到100%.将高约3 cm的丛生芽切下,接种于分别添加0.5 mg/L IAA或0.5 mg/L IBA的1/2 MS培养基上可产生不定根,获得完整的再生植株.

Abstract:With tomato seedling leaves,variety 'Gailiang America 908' and 'Hezuo 906' as material,polyphenol oxidase(PPO) and peroxidase(POD) activity were measured after sprayed with salicylic acid and acetylsalicylic acid within 120 h.The results indicated that th

Abstract:以津春3号黄瓜为试材,对其喷施0.05、0.1和0.5 mmol/L的水杨酸(SA)溶液,并进行了昼(40±1)℃/夜(30±1)℃高温胁迫处理,研究了黄瓜幼苗四叶期叶片叶绿素荧光参数变化.结果表明,高温胁迫下幼苗叶绿素荧光参数Fo、NPQ等显著升高,Fv/Fm、Fv′/Fm′等明显降低,嫁接苗的变化趋势弱于自根苗;不同浓度的SA溶液可缓解高温胁迫对黄瓜幼苗叶绿素荧光参数的影响,且以0.1 mmol/L的SA处理效果最优.

Abstract:Sodium nitroprusside(SNP),used as the donor of nitric oxide,was added to the suspension cells of Ginkgo biloba L.The aim is to investigate the effects of exogenous nitric oxide on flavonoids production,PAL and CAT activity and the growth of suspension cel

Abstract:The response of leaf photosynthetic characteristics and antioxidation were studied with Pot experiment under drought and rewatering condition in six wheat genotypes.Compared with drought treatment after rewatering wheat at erect seedling stage,the photosy

Abstract:Studies on the behavior of seed germination and plant growth and development of Xinjiang ephemeral plant Arabidopsis pumila under salt-stress were carried out.The results showed that(1) seed germination was inhibited during NaCl stress,but partial seeds w

Abstract:Effects of enhanced UV-B radiation on physiological and biochemical characters of tobacco were investigated.The results showed that the photosynthetic pigments(chlorophyll a,chlorophyll b and carotenoids) increased clearly in tobacco leaves under enhanced

Abstract:采用5种不同的水分处理,于室内生长箱内盆栽条件下对引种禾草柳枝稷(Panicum virgatum)、乡土禾草白羊草(Bothriochloa ischaemum)和栽培禾草谷子(Setaria italica)等苗期根冠生长和水分利用特征进行了比较分析.结果显示:(1)充分供水下3种禾草的苗期生物量和蒸腾效率均显著高于其它4种水分处理,而高水与低水处理下各自的根冠比无显著差异;(2)各水分处理下谷子苗期总生物量、蒸腾效率和耗水量均显著大于白羊草和柳枝稷;(3)在5种水分处理下,苗期根冠比谷子均最小,柳枝稷最大;(4)低降复水后,3种禾草生物量和蒸腾效率较低水处理分别显著提高16.7%-98.7%和28.2%-118.2%,显示出补偿效应,以白羊草增幅最大.结果表明,白羊草、谷子和柳枝稷在不同土壤水分处理下的苗期生物量、根冠分配比例以及水分利用效率差异反映了野生种、栽培种和引进种禾草苗期对半干旱环境条件水分生态适应性的异同.

Abstract:对麦秸翻压还田后隔茬冬小麦根系活力、旗叶叶绿素含量、丙二醛(MDA)含量及光合速率等衰老指标进行了测定分析.结果显示,(1)小麦秸秆还田后可显著促进各时期根系的生长并显著提高小麦根系活力,且根系在30cm处土壤中的分布增加;(2)冬小麦生育后期叶片叶绿素以处理Ⅲ(6 000 kg/hm2)的降解速度(18%)最慢,旗叶光合速率在灌浆期和蜡熟期以处理Ⅲ为最高,旗叶丙二醛含量以处理Ⅲ最低,比对照(Ⅰ)低6.78 mmol·g-1FW,丙二醛含量与光合速率呈显著负相关.研究结果表明,适量秸秆还田对接茬玉米的产量影响不显著,但可显著延缓隔茬冬小麦的衰老并显著提高产量,在黄土高原有灌溉条件的地区以6 000 kg/hm2的小麦秸秆量还田较适宜.

Abstract:Activities of nitrogenase,SOD,POD and CAT of intercropped peanut(IP) and pure-planted peanut(PP) during yield-forming stage were studied with wheat cultivar 'Yannong 23' and peanut cultivar 'Huayu 22' in field.The main results were described as follows th

Abstract:The sap flow rate of Populus euphratica and relative environmental factors have been observed for 3 days at Yingsu in the lower reaches of Tarim river by advanced PHYTALK system.The results showed the following regularities:the diurnal variation of sap fl

Abstract:选择位于水蚀风蚀交错带强烈侵蚀中心的神木六道沟流域,通过分层挖掘法调查了6种典型人工植物细根垂直分布特征.结果表明;(1)各树、草种的细根垂直分布特征具有相似性,即浅层土壤中细根分布较多,深层土壤少;(2)细根密度(Fine root density,FRD)垂直分布存在较大差异,0-2 m土层刺槐(Robinia pseudoacacia)总FRD为1.195 m2·m-2,柠条(Caragana korshinskii)为0.927 m2·m-2,沙柳(Salix psammophila)为0.941 m2·m-2,沙棘(Hippophae rhamnoides)为1.248 m2·m-2,沙蒿(Artemisia ordosica)为0.446 m2·m-2,紫花苜蓿(Medicago sativa)为0.631 m2·m-2;(3)细根垂直分布与土壤水分分布存在显著相关性.

Abstract:Genetic variations and systematic relationships of nine populations of Elymus nutans from Qinghai-Tibetan were determined using allozyme markers.Allozyme data indicated that genetic distance in the nine populations ranged from 0.010 6 to 0.377 8.Genetic d

Abstract:The intraspecific and interspecific competition intensity of Quercus liaotungensis in Malan forest region of Loess Plateau were quantitatively analyzed using Hegyi's single tree competition index model.The results showed:(1) The intensity of the intraspec

Abstract:The species diversities,community-structure dynamics,and soil-water and-nutrient effects of 30-year-old Populus simonii plantations,which were located on north-facing slope,half-north-facing,half-south-facing and south-facing slopes in hilly area of the L

Abstract:在野外调查和查阅文献资料的基础上,研究了七姊妹山自然保护区国家重点保护野生植物的分布情况,并运用濒危系数、遗传价值系数和物种价值系数对其优先保护顺序进行了定量分析.结果表明:(1)七姊妹山自然保护区共有国家重点保护野生植物28种,其中Ⅰ级7种,Ⅱ级21种,分别占湖北省分布的60.9%、87.5%和55.30%;(2)采用优先保护评价体系对28种国家重点保护植物进行排序,其结果与《国家重点保护野生植物名录(第一批)》结果有所不同,属一级保护的有银杏和伯乐树2种,占总数的7.14%;属二级保护的有桢楠、连香树、光叶珙桐、红豆杉、珙桐、黄杉、花榈木、篦子三尖杉、毛红椿、巴山榧树、水青树、红豆树、闽楠、南方红豆杉、莼菜、红椿和川黄檗17种,占总数的60.71%;属三级保护的有喜树、香果树、鹅掌楸、榉树、厚朴和凹叶厚朴6种,占总数的21.43%;属四级保护的有金荞麦、樟树和野大豆3种,占总数的10.71%;(3)该评价体系对28种植物的评价与分级结果更真实地反映了它们在该区域受威胁状态和优先保护顺序,对该区野生重点保护植物的优先保护工作具有一定指导意义.

Abstract:Dashanfeng,located in the south of Zhejiang,is one of the richest area of Zhejiang in woody plant flora.543 woody species(including subspecies,varieties and forms) belong to 237 genera in 84 families.There are abundant ancient,relict,rare and nearly exter

Abstract:从秀雅杜鹃全草的石油醚部分和乙酸乙酯部分中分离出20个化合物,利用现代波谱技术(MS1、HNMR、13CNMR、DEPT、CD)和化学方法对其中17个化合物进行了结构鉴定,分别为2S-4′,5,7-三羟基黄烷酮(1)、(2R,3R)-( )花旗松素(2)、扁蓄苷(3)、槲皮素-3-O--αL-鼠李糖苷(4)、金丝桃苷(5)、槲皮素(6)、16,17-二羟基,11β-贝壳杉烷-19酸(7)、异莨菪亭(8)、喇叭茶醇(9)、豆甾-4-烯-6β-醇-3-酮(10)、β-香树脂醇(11)、α-香树脂醇(12)、28-羟基-β-香树脂醇(13)、山楂醇(14)、齐墩果酸(15)、β-谷甾醇(16)、β-胡萝卜苷(17).所有化合物均为首次从该植物中分得,其中化合物1、7、8、9、10为首次从该属植物中发现.

Abstract:选用春、秋两季的午子绿茶样品,采用固相微萃取法提取、富集其芳香物质,经GC-MS分析,共鉴定出112种化合物,其中春茶102个组分、秋茶96个组分.不同生产季节绿茶中香气物质构成种类基本相同,其含量分布存在差异.苯乙醇、苯甲醇、2,6-二叔丁基对甲苯酚、壬醛、咖啡因、香叶醇、β-芳樟醇、1,2,4a,5,8,8a-六氢-4,7-二甲基-1-异丙基萘等化合物作为午子绿茶中主要的香气物质,对于构成其特征风味具有重要作用.

Abstract:从菊科植物大花金挖耳（Carpesium macrocephalum）的花、茎、叶、果实和根中分离出92株内生菌,其中真菌68株,细菌16株,放线菌8株;对峙法生物活性测定结果表明,其中22株内生菌分别对小麦赤霉病菌、黄瓜灰霉病菌、辣椒疫霉病菌、玉米大斑病菌和苹果炭疽病菌均有较好的拮抗作用;采用生长速率法测定22株内生菌发酵产物的抑菌活性,结果表明,内生细菌H-32的发酵液稀释10倍和内生真菌H-18、Y-12、G-4、G-6的菌丝丙酮提取物在0.5 mg·mL^-1（菌丝干重）的剂量下,均对番茄灰霉病菌和玉米大斑病菌菌丝生长具75%以上的抑制效果;活体组织法测定结果,H-32的发酵液和H-18、J-1、Y-17、G-6的菌丝丙酮提取物在2.0 mg·mL^-1（菌丝干重）的剂量下,对番茄灰霉病的防治效果均在50%以上.综合分析认为,H-32、H-18和G-6等内生菌的抑菌作用值得进一步研究.

Abstract:The polysacchrides are the one of the main bioactives in the roots of Huang-jing(Polygonatum sibiricum Red.).By measuring the dry matter weight,drying ratios,extractable matter contents and sacchride contents,we evaluated the dry matter accumulation and t

Abstract:Saposhnikovia divaricata(Turcz.) Schischk.is chosen as the material to study the distribution of starch in cultures by semithin section and the content of polysaccharides by spectrophotometry in the different stages of somatic embryogenesis.The results sh

Abstract:During the major onset of downy mildew,The PPO and POD activities in leaves were determined in three grapevine varieties(lines),8804,Merlot and Cabernet Franc..It was shown that the PPO and POD activities kept higher for a longer time with variety 8804 an

Abstract:Glomus fasciculatum Gerd.& Trappe emend.Walker and Koske,Glomus constrictum Trappe were separately and/or mixed inoculated on seeding of Forsythia suspensa(Thunb.)Vahl.The effects of arbuscular mycorrhizal fungi on drought resistance were studied in this

Abstract:Phytosulfokine,a sulfated pendapeptide widely existed in plant kingdom,has shown mutiple biological activities besides proliferation of single cells.The discovery of phytosulfokine,the relationship between structure and function,biological activites,biosy

Abstract:The structure and growth characteristics of pollen tubes have been focused on for centuries.As the development of biology,discovery of new material and application of new fluorescent label,great progress has been made in the field of pollen tubes' growth

Abstract:Both the effects of salinity on ecology research of arbuscular mycorrhizal fungi(AMF) and growth effect of AMF on plants were depicted.The progresses in physiology and molecular mechanism of salt tolerance induced by AMF were reviewed in this paper,which

Abstract:Under temperature stress,a series of proteins named low-temperature-induced proteins are produced in plants,which mainly include AFPs,DHNs and HSPs.Once they produced in plants,the plants can have strong tolerance to stresses and adapt to the stress condi