Abstract:

Abstract:According to problem existing in the traditional paraffin section preparation,we made some improvements about the Usnea's manufacture of paraffin section.With Usnea betulina Mot.from high elevation areas as the subject,based on the research of material se

Abstract:以银腺杨、毛新杨和银毛杨为材料,采用定期授粉观察以及联苯胺-过氧化氢染色等方法,对白杨派树种雌蕊柱头可授性进行了研究,结果表明:在温室水培条件下,白杨树种雌蕊柱头的可授期一般在1~3 d内,其中毛新杨雌蕊柱头可授期比银腺杨和银毛杨相对短些.但3个树种进入最佳授粉时期时的柱头开裂角度约为180°,此时柱头发亮且表面有大量分泌物存在;在适宜授粉时期内,用联苯胺-过氧化氢染色后柱头变蓝,并有气泡产生。在育种实践中,可根据柱头形态以及联苯胺-过氧化氢染色后柱头颜色变化和气泡产生情况及时判别有关白杨派树种最佳授粉时期。

Abstract:By means of paraffin section and microscopic observation of alder cuttings,the secondary structure of stems and the process of adventitious root development was dealt with in this paper,in order to understand the factors affecting adventitious root events

Abstract:根据对中国野生葡萄华东葡萄株系白河-35-1在白粉病诱导下构建的cDNA文库中获得的1条EST序列设计特异引物,以总RNA逆转录产物为模板,进行SMART-RACE扩增。结合生物信息学方法对所获的序列进行开放阅读框、序列同源性分析,并预测了蛋白质的理化性质、信号肽、酶与非酶分析、亚细胞定位以及蛋白质二级结构。结果表明,获得的中国野生葡萄新基因的全长序列为854 bp,其开放阅读框为585 bp,5′非编码区为107 bp,3′非编码区为162 bp;同源性比对表明该新基因推导的氨基酸序列与拟南芥RNA结合蛋白和水稻推定的半乳糖基转移酶的同源性分别为55%和63%,第8~82位氨基酸序列与RNA识别基序相类似,是一个典型的结构功能域;推导该基因表达产物为相对分子质量为21 801.27、等电点为6.86的不稳定蛋白,定位于细胞核,不包含信号肽,二级结构主要是无规则卷曲。

Abstract:AGPase gene isolated from Escherichia coli BL21 was constructed into a plant expression vector named pCB-C336 and thus it was made to express itself in transgenic tobacco plants.The results indicated that the construction of the AGPase gene plant expressi

Abstract:Using specific primers based on common wheat cultivar Chinese spring ESTs,which are homologous to CEN,TFL1 in Antirrhinum and Arabidopsis respectively,we obtained a fragment of wheat CEN/TFL1 like gene from total RNA of young inflorescence of 'Chinese Spr

Abstract:Based on the leaf cDNA library of Leymus chinensis,we used the M13 universal primers to screen positive clones to sequence.The sequences were submitted to NCBI for estimating their homogeneity,and we got a Rubisco large subunit gene and a gene fragment of

Abstract:根据已获得的盐生植物獐茅（Aeluropus littoralisvar.sinensisDebeaux）液泡膜Na^＋/H^＋逆向转运蛋白（Na＋/H＋antiporter）基因部分cDNA片段,设计2条特异引物（GSP1、GSP2）,采用RLM-RACE（RNA ligase-medi-ated rapid amplification of 5′and 3′cDNA ends）法获得了其5-′cDNA末端序列,并找出了转录起始位点。该片段长816 bp,与芦苇液泡膜Na^＋/H^＋逆向转运蛋白基因序列同源性最高达91%,与拟南芥、盐角草、水稻、大麦、小麦的同源性分别为81%、82%、86%、87%和81%,为该基因全长的克隆及启动子的研究奠定了基础。与其它测定基因转录起始位点的方法相比,RLM-RACE方法更快捷、简便、准确。

Abstract:A partial cDNA,encoding a protein homologous to phytoene synthase(PSY),has been isolated from watermelon fruit,using a pair of primers designed from conservative regions of other plant PSY proteins.The sequencing result showed that the length of the cDNA

Abstract:The mature embryos and the immature embryos chosen from wheat cultivars,'Xiaoyan 22' and 'Ningchun 16',were cultured to study the effect of different kind of embryos and various factors on callus induction and differentiation.The results showed that there

Abstract:Six cultivars of strawberry(Fengxiang,Guilugan,Quanmingxiang,Zhangji,Hali,Xingxiang)were investigated for factors affecting the regeneration of adventitious bud.The results indicated that the genotype,hormones and seedling ages were the main factors affec

Abstract:The genetic character of foreign antisense-trxs gene and debranching enzyme activity in transgenic line 01TY34-73-9 were detected by PCR and enzyme activity.The results showed that the anti-trxs gene had been inherited to T4 generation.The results of anal

Abstract:To study the taxonomy of 'Luobuma',3 non-coding regions of DNA sequences(nuclear ribosomal ITS,chloroplast trnL intron,and trnL-F region) of Apocynum venetum,Poacynum pictum and P.hendersonii from China were sequenced and analyzed together with other rela

Abstract:对不同来源的8份白芥材料,采用人工自交法分析其自交亲和性。结果显示:白芥的自交亲和性存在较大幅度的变异,自交亲和指数在0.01~4.10之间,8份参试材料中,自交亲和指数小于1的材料有3个,自交亲和指数大于1的材料有5个。表明白芥中存在自交亲和材料,白芥自交亲和性变异不仅存在于材料间,而且也存在于同一材料内不同个体间。按自交亲和指数的高低,可将参试材料分为3种类型:高自交亲和类型(自交亲和指数大于3.00,如民乐洪水芥麻、04(X)等)、自交亲和类型(自交亲和指数为1.00~2.99)、自交不亲和类型(自交亲和指数0.00~1.00)。

Abstract:The antioxidant activities of the extracts from Fructus lycii under different extraction conditions were measured by the methods of Folin-Ciocalteu,DPPH assay and scavenging action to hydroxyl free radical.The same tendency is observed.The extracts of the

Abstract:The study probed into dynamic variations in the chlorophyll contents,net photosynthetic rates and SOD,CAT and POD activities,and MDA contents of the leaves of two mung bean varieties,Jilu 2 and Tailai at different flower-set caulis nodes from flowering to

Abstract:对热带植物小芸木的种子和胚在整个发育阶段的形态学特征、含水量、萌发率和电导率进行了研究。结果显示:(1)小芸木种子在发育过程中形态学特征、电导率、萌发率和胚的脱水耐性有明显的变化;(2)在55~80 d种子鲜重和干重逐渐增加,随后又稍微降低,整个发育过程未经历明显的成熟脱水阶段;种子萌发率从55~85 d逐渐达到最大,随后又稍微降低;(3)胚的脱水耐性从55~90 d逐渐增加,于85~90 d达到最大,在95~103 d时又有所下降。表明小芸木胚的最大脱水耐性的获得时间与种子干物质积累达到最大的时间一致。

Abstract:采用营养液栽培,以根际低氧耐性不同的2个黄瓜品种为试验材料,研究了Ca2 对根际低氧胁迫下黄瓜幼苗生长和叶片荧光特性的影响。结果表明:(1)根际低氧胁迫下,黄瓜植株根长、根表面积和根尖数减少,但根径有所增大;叶片干重、鲜重和叶面积显著减小,提高营养液钙浓度可使植株叶片干、鲜重和叶面积得到部分恢复。(2)根际低氧胁迫下,叶片光合色素含量降低,提高营养液钙浓度对色素含量无明显影响。(3)根际低氧胁迫下,常钙和高钙处理黄瓜叶片Fv/Fm与通气常钙(CK)无显著差异,但低氧缺钙处理的Fv/Fm显著降低;与通气常钙相比,根际低氧胁迫处理的光化学猝灭(qP)减小、非光化学猝灭(qN)增大、光合功能相对限制值(L(PFD))升高,提高营养液钙浓度可使qP和qN恢复至近对照水平,而使L(PFD)低于对照,且‘绿霸春四号’黄瓜品种表现得更为突出;根际低氧胁迫下,光化学速率(Prate)减小,天线热耗散速率(Drate)都随钙浓度升高而降低。总之,根际低氧胁迫下黄瓜幼苗生长被显著抑制,PSⅡ反应中心受到一定程度的破坏,提高钙浓度可使PSII反应中心恢复至接近甚至高于通气对照的水平,从而有效缓解根际低氧胁迫对黄瓜幼苗造成的伤害。

Abstract:Electrical resistance and reactance of wheat leaves were researched,which are attributed seven varieties such as dryland,irrigated area and dry-fertilizer.The wheat were growed in pots experienced three water conditions:optimization moisture,moderate drou

Abstract:通过伤害处理和外施诱导子——水杨酸(SA)、茉莉酸甲酯(MeJa)和乙烯利(Ethrel)以及伤害和诱导子复合处理,研究不同处理对喜树中喜树碱(camptothein,CPT)含量的影响。结果表明:(1)4种单独诱导处理均能在一定程度上增强喜树碱的合成,其中水杨酸处理和机械伤害诱导效果最明显,较对照组的喜树碱含量分别提高了47.7%和27.2%,而机械伤害和诱导子复合处理均产生拮抗作用,喜树碱含量降低;(2)诱导作用受诱导剂浓度的影响,SA、MeJa和Ethrel的适宜质量浓度分别为50~100 mg.L-1、5~10 mg.L-1和5~10 mg.L-1。

Abstract:用柱层析和高效液相色谱等方法从卫矛科卫矛属植物冬青卫矛(Euonymus japonicusThunb.)的根皮乙酸乙酯提取物中分离到4个β-二氢沉香呋喃大环生物碱,它们的结构经核磁共振波谱分析和熔点测定鉴定为卫矛羰碱(evonine)(A),1-脱乙酰-1-苯甲酰卫矛羰碱(B),ebenifoline E-IV(C)和mayteine(D)。生物活性追踪试验表明,化合物A、B、C、D对粘虫(Mythimna separata)幼虫的毒力LC50分别为23.23、5.44、5.25和4.66 mg.mL-1。

Abstract:The edge effect of plant communities was investigated in a wetlanddry grassland ecosystem at Siertan wetland in Yanchi county,Ningxia.Four transects lines each about 1 km long were set up in July 2005 along the biotope gradient in four directions:east,nor

Abstract:应用主成分分析方法研究了阿拉善荒漠区白沙蒿飞播区土壤因子和白沙蒿生长状况,阐明了土壤性质的主要限制性因子和群落生长最主要的状况指标。结果表明:(1)各个土壤因子中,6月的土壤含水量是各土壤性质中最重要的因子,贡献率达到63.6%。土壤的分形维数也对土壤性质有重要作用,贡献率为8.8%。土壤中N、P、K含量的贡献率分别为5.4%、3.5%、3.5%,也是不容忽视的影响因子。(2)地上生物量最能反映植株的生长状况,其贡献率达到60.1%,而且和几种最主要的土壤因子都有极高的相关性。(3)对土壤因子的第一和第二主成分的排序结果显示,不同白沙蒿种群密度分为3个类型,而种群密度在2.1株/m2时,种群生长状况最佳。

Abstract:Ten correlative indexes such as Shannon-Weiner index of the arbor,shrub and herbaceous,the quantity proportion of seedling,saplings and adults,humus thickness,and so on,which could describe the community stability,were chosen in order to reveal the relati

Abstract:对河西走廊荒漠植物的群落类型、盖度、密度、重要值、地上生物量等进行了调查,收集整理各区域的气候资料,对它们的关系进行了初步研究。结果显示:(1)物种种类组成、总密度与海拔、经度呈正相关,与纬度呈负相关,在走廊的东南→西北方向上呈递减趋势。(2)在活动积温(>10℃)>3 200℃的荒漠地区,灌木(包括半灌木)在群落中占有绝对优势,其重要值可达到100;当活动积温在3 000℃左右时,群落往往是一些过渡类型,或以草本为优势种、以半灌木或灌木为伴生种,或以半灌木为优势种、以多年生草本为伴生种。(3)在年降水量110 mm以上的荒漠地区,植物群落生活型主要为半灌木或多年生草本;在年降水量110 mm以下的地区,生活型主要为小灌木或一年生草本;在年降水量小于50 mm的极端干旱地区,只有在雨季时有一年生草本生长。表明荒漠生态系统植被稀疏,生物量小,对气候因子,特别是降水变化敏感,对其保育显得尤为重要。

Abstract:The genus Viola subgen.Melanium in China was revised and 6 species were recognized.A key to species and descriptions to the three species that haven't been included in the Flora Reipublicae Popularis Sinicae(Vol.51) were given,and the drawings of Viola ta

Abstract:用环境扫描电子显微镜对沉水植物长梗苦草(Vallisneria longipedunculata)的雄花结构进行了观察研究。结果显示,长梗苦草雄花具3枚花被,2枚雄蕊是由3枚雄蕊中的1枚退化而来的,退化雄蕊清晰可见;花药4室,发育过程中通过药室顶端开裂,以及花药室基部的细胞收缩,将花粉粒都推到花药室的裂口处进行发育,成熟花粉粒直接外露;长梗苦草的花粉粒直径约50μm,壁薄,表面几乎没有纹饰,未发现萌发孔或萌发沟,这些是对特殊生境和传粉方式的适应。

Abstract:采用正交实验设计(L934)用MS、White和改良MS培养基,分别加入不同质量浓度的玉米素(ZT)、6-苄基氨基腺嘌呤(6-BA)、异戊烯基腺嘌呤(2-ip)进行苦参组培快繁技术体系研究。结果表明,以苦参种子萌发的幼苗为外植体效果较好。3种基本培养基对诱导芽增殖效果差异不显著;在基本培养基中分别加入6-BA、2-ip和ZT诱导芽增殖,但3种细胞分裂素之间差异不显著。培养基中含2 mg/L 6-BA(或ZT或2ip)增殖效果最好,其次是3mg/L的细胞分裂素;以改良MS 2 mg/L 6-BA是苦参较好的增殖培养基;组培苗在1/2 MS 1.5 mg/L IBA培养基上根诱导效果最好(98%),移栽到珍珠岩 蛭石基质(1∶1)中成活率最高(96%)。

Abstract:采用不同浓度赤霉素(GA3)处理臭椿种子,在不同温度及不同光照条件下,研究了GA3、温度及光照对臭椿种子萌发的影响。结果表明,40 mg.L-1的GA3质量浓度、30℃温度以及自然光暗交替处理为臭椿种子萌发的最适条件,臭椿属于喜高温型种子。

Abstract:The fatty acids from the seeds oil of Decaisnea insignis and Akebia trifoliata were extracted with petroleum ether by Soxlet extraction.The extracted fatty acids were esterified and then analyzed by gas chromatographymass spectrometry.Nine fatty acids amo

Abstract:Some species of the genus Davallia are used as Gusuibu,a famous traditional Chinese Medicine.The chemical constituents and bioactivity of the genus were reviewed and compared with that of Drynaria fortunei,the sole source plant of Gusuibu recorded in Chin

Abstract:Chromosome microdissection and microcloning technology was first used in animal and human.With the development of PCR,they was applied in plant genetic and evolutional researches widely.This article summarized the principle of the technology,its major pro

Abstract:The Qinghai-Tibetan plateau possesses the largest area of natural grasslands in China.The grassland ecosystem on the plateau is hypothesized to play an important role in the carbon balance in both regional and global ecosystems.It is therefore of great si

Abstract:Water stress is one of the major environmental stresses that limit plant growth and development.Plants can respond and adapt to water stress by perceiving the stimulus,generating and transmitting the signals,and initiating various defense mechanisms.Absci