Abstract:To investigate the expression of foreign fusion gene of hepatitis B surface antigen and the activation of the translated protein,three different fusion HBsAg genes,LP PreS1 PreS2 S,PreS1 PreS2 S and PreS2 S,were cloned into a potato virus X(PVX) expressio

Abstract:According to the reported sequence of CBF1 gene(GenBank Accession No.AF376136) in wheat(T.aestivum),the primers specific to the gene were designed and constructed to clone the gene from 'Jinhua No.1'(T.aestivum) and sequence it by PCR.Expression vector pB

Abstract:以‘条中32’接种前后的小麦抗条锈病种质‘陕麦139’幼苗叶片为材料,利用抑制消减杂交技术,构建了条锈菌接种48 h小麦抗病品种叶片的抑制表达SSH-cDNA文库,通过测序以了解条锈菌侵染后被抑制表达的相关基因。从构建的文库中随机选取48个阳性克隆,进行测序,经过序列拼接等,获得高质量EST序列34条(Gen-Bank序列号为EL930132-EL930165),序列比对分析表明其中31条EST序列与已知功能的基因序列同源性较高,主要涉及植物的能量代谢途径、膜转运、信号传导及核酸加工等方面的基因,包括光合系统Ⅱ抗性蛋白D1、碳酸酵素、线粒体磷酸盐转运子、苹果酸脱氢酶、过敏性诱导反应蛋白和rRNA核酸内切酶等相关基因。此外,获得未知功能的EST序列3条。研究表明,小麦在条锈病菌侵染初期,许多基因的正常表达调控受到影响,如能量代谢和寄主细胞结构木质化,阻止小麦抗病过敏性反应等。

Abstract:以非单性结实的全雌黄瓜为实验材料,利用cDNA-AFLP技术比较了授粉前后黄瓜幼果的基因表达差异。以ASE-AT/TAQ-CAG为引物对从授粉后黄瓜幼果组织中分离到1条特异片段,该片段仅在授粉后黄瓜幼果组织中表达,将该片段回收测序并翻译成氨基酸序列,用blastp程序在NCBI GenBank数据库中进行同源性检索和相似性比对,结果发现该片段推导的氨基酸序列与黄瓜扩张蛋白CsEXP1~9的相似性依次分别为71%、58%、63%、75%、85%、82%、67%、68%和85%,可能是一个新的黄瓜扩张蛋白基因,命名为CsEXP10,表明扩张蛋白基因可能与黄瓜果实膨大生长有密切关系。

Abstract:The selection effects of the growth and regeneration of barley callus and the effects of barley seed of 'B1202','U202' and 'Yunyin 1' treated with hygromycin in different concentration were studied.The results showed that the optimal concentrations of hyg

Abstract:以桂花(Osmanthus fragrans Lour.)的休眠芽?萌动芽和嫩枝的茎尖为外殖体,进行离体培养研究,筛选其最适培养基。结果表明:3种外殖体的适宜初代培养基不同,其中:休眠芽为B5 7.0 mg.L-16-BA 0.05 mg.L-1NAA 3%蔗糖;萌动芽为B5 5.0 mg.L-16-BA 0.05 mg.L-1NAA 3%蔗糖;嫩枝为B5 3.0 mg.L-16-BA 0.05 mg.L-1NAA 3%蔗糖;但适宜的继代培养基(B5 2.0 mg.L-16-BA 0.05 mg.L-1NAA 3%蔗糖)和生根培养基(B5 2.0 mg.L-1NAA 3%蔗糖)相同。

Abstract:The genetic diversity and relationship of 58 accessions of Zoysia Willd.belonging to 9 species and 1 variety were analyzed using SSR markers.267 bands were amplified from 9 species and 1 variety by 14 pairs reliable SSR primers.A high level of genetic div

Abstract:The somatic chromosome numbers and karyotypes of 4 species embracing 6 populations from China were investigated by conventional pressed disc method.The results indicated that the chromosome numbers of these species were 2n=22 or 2n=44 and karyotypes of al

Abstract:The different methods of the pretreatments and dissociations in chromosome sectioning of Schisandra sphenanthera Rehd.et Wils.were compared,and different parts,including buds,top of stems,and young leaves,were used to chromosome sectioning,500 cells were

Abstract:The floral morphogenesis of the Aristolochia contorta Bge.was observed with a scanning electron microscopy(SEM).In A.contorta,the initiation of the sepal resembles to that of bract,four of the six stamens initiate firstly and the other two slightly later,

Abstract:A comparable study on macro-and micro-morphology of Astragalus leansanicus and A.limprichtii was carried out.The similar morphology of tubular calyxes tube and linear pods in both species indicates the two should be attributed to the same section,supporti

Abstract:The leaf epidermis structures of 27 species,which belong to Osmanthus have been examined under LM and SEM.The stomatal size,stomatal density,stomatal index and gland density,shape and pattern of anticlinal walls of epidermis cells were measured and counte

Abstract:The development of perispore in the fern of Dryoathyrium coreanum(Christ) Tagawa is studied by means of light microcope(LM),scanning electron microscope(SEM) and transmission electron microscope(TEM).The results as follow:the mature spores of D.coreanum a

Abstract:Gravity slide method,bagged and TTC pollination were adopted to study the pollen-shedding rule,breeding system,pollen vitality,pollination countermeasure of Paris verticillata and its flower characteristic versus conform to anemophilous speciality.The res

Abstract:以发菜为材料,比较了提取液类型和饱和硫酸铵浓度对藻蓝蛋白提取的影响,并对藻蓝蛋白的提取程序和部分特性进行了研究。结果表明:50 mmol/L KP缓冲液(pH值7.2)是合适的提取液,体积分数为40%~50%饱和硫酸铵盐析效果优于其它浓度。经过DEAE-Toyopeal 650 S离子交换层析和SuperdexTM200凝胶过滤层析后,藻蓝蛋白纯度达6.2,最大吸收峰位于615 nm,荧光发射峰位于649 nm,由α和β2个亚基组成,其分子质量分别为18 051.17和19 142.27 Da。因此,发菜藻蓝蛋白分离纯化较为理想的程序为:藻粉→50 mmol/L KP缓冲液(pH值7.2)浸泡→French pressure(1 500 kg/cm2)破碎细胞→40%~50%饱和硫酸铵盐析→DEAE-Toyopeal 650 S离子交换层析→SuperdexTM200凝胶过滤层析→较纯的藻蓝蛋白。

Abstract:The leaves of Rabdosia rubescens(Hemsl.) Hara were used as the explants to study the callus induction and plant regeneration on the MS media containing different hormone combinations.On the basis of it,leaves and stems of R.rubescens were used as explants

Abstract:从除虫菊(Pyrethrumcinerariifolium Trev.)的根、茎、叶和花中分离出128株内生真菌。生长速率法测定结果表明,有56株内生真菌发酵液10倍稀释液至少对供试的番茄灰霉病菌(Botrytis cinerea)、辣椒疫霉病菌(Phytophthora capsici)、苹果炭疽病菌(Colletotrichum gloeosporioides)、玉米大斑病菌(Exserohilumturcicum)、小麦纹枯病菌(Rhizoctonia cerealis)和小麦赤霉病菌(Fusarium graminearum)等6种病原菌中的一种抑菌活性达75%以上;活体组织法测定结果,Y1、Y2、H2共3株菌的发酵液对番茄灰霉病的防效达到50%以上;盆栽试验结果表明,Y1、Y2、Y7、H2、G13共5株菌株发酵液对辣椒疫霉病的防治效果达到50%以上。综合分析认为,Y1、Y2、H2、G13等内生真菌的抑菌作用值得进一步研究。

Abstract:以不同浓度的大蒜秸秆水浸液对西瓜枯萎病菌、黄瓜枯萎病菌、辣椒疫霉病菌进行抑菌圈试验,并对3种不同的大蒜秸秆萃取液中的可挥发抑菌物质进行鉴定,以了解大蒜秸秆水浸液的抑菌作用。结果表明,大蒜秸秆水浸液对3种靶标菌均有抑菌活性,其最有效的抑菌浓度为100 g.L-1,对辣椒疫霉病菌的抑菌活性最强。大蒜秸秆水浸液的石油醚、三氯甲烷和乙酸乙酯3种有机溶剂萃取相对3种靶标菌均有抑菌活性,以乙酸乙酯相抑菌活性最强,在50 g.L-1浓度下对辣椒疫霉菌抑菌率达100%,在100 g.L-1浓度下对西瓜枯萎病菌和黄瓜枯萎病菌的抑菌率也达100%;三氯甲烷相的抑菌活性稍高于石油醚相;抑菌活性基本随浓度增加而增大。经GC-MS分析,初步确定大蒜秸秆水浸液中的可挥发抑菌物质主要有2-甲氧基-1,6-己二酸甲酯、邻苯二甲酸二丁酯等有机酯类,3-甲氧基-4-羟基-苯甲酸、对羟基苯甲酸等有机酸,以及对甲氧基苯酚等酚类化合物。

Abstract:通过水培试验,研究了模拟酸雨(pH 3.5)与Al(3、30 mg/L)、Pb(25、250 mg/L)、Cd(0.11、mg/L)单独或复合胁迫对小麦幼苗生长及抗氧化酶活性的影响。结果表明,模拟酸雨与Al、Pb、Cd单独或复合处理12 d后,小麦幼苗根长降低54.01%~94.81%,苗高降低18.88%~77.95%,单粒萌根数降低21.87%~80.37%,其生长发育受到显著抑制,抑制效应随处理浓度增大和作用时间延长而增强,且复合处理组抑制效应更强;幼苗根部超氧化物歧化酶(SOD)活性在模拟酸雨与单一金属离子处理下显著升高,而在3种金属离子复合处理下显著降低,但酸性条件下无论金属离子单独及复合处理后,过氧化物酶(POD)活性均显著升高,过氧化氢酶(CAT)活性均明显下降。研究发现,酸性条件下3种金属离子对植物细胞均产生氧化胁迫作用,且它们复合胁迫的毒性效应大于其单独胁迫。

Abstract:以‘大西洋’、‘夏波蒂’2个品种的脱毒微型种薯为试验材料,分别在冷藏(4~6℃)、窖藏(8~12℃)和常温(18~22℃)条件下贮藏110 d,研究不同贮藏条件和温度对马铃薯微型种薯活力及生理特性的影响。结果表明:(1)低温冷藏能显著提高微型种薯的活力,2个品种的活力指数分别比窖藏和常温贮藏高20.44%、20.28%和33.51%、35.46%。(2)冷藏与窖藏和常温贮藏相比,CAT活性高35.37%~48.25%、91.68%~125.23%,SOD活性高5.79%~6.60%、10.53%~12.81%,而MDA含量却降低30.47%~47.79%、85.54%~87.09%。(3)随着微型种薯的重量增加其活力指数显著提高,CAT和SOD活性也增加,而MDA含量逐渐降低。(4)经不同条件贮藏后,微型种薯的含水量呈下降趋势,以冷藏条件的含水量降低最少;可溶性蛋白含量显著增加,冷藏比窖藏和常温贮藏分别高14.63%~24.67%和25.5%~35.04%;淀粉含量表现为不同程度降低,以窖藏降低最少;可溶性糖含量虽有明显降低,但其含量变化无显著差异。说明低温贮藏可以使马铃薯微型种薯保持较高的活力,适合贮藏条件的顺序依次为:冷藏>窖藏>常温贮藏。

Abstract:采用Li-6400光合分析仪,在自然条件下对不同坡位胡枝子叶片净光合速率(Pn)、蒸腾速率(Tr)及其相关环境因子的日进程进行了观测。结果表明:在5月份晴天条件下,不同坡位胡枝子叶片Pn、Tr日变化均呈单峰曲线,而水分利用效率(WUE)表现出早晚高、中午低的变化趋势。它们的叶片Pn峰值不同坡位均出现在11:00,而且上坡位峰值和日平均值略高于下坡位和平地(P>0.05);Tr峰值上坡位出现在11:00,下坡位和平地均出现在13:00,且上坡位峰值和日平均值显著低于下坡位和平地(P<0.01);上坡和平地WUE均在15:00达到最大值,但下坡位推迟到17:00,而且上坡位峰值和日平均值略高于下坡和平地(P>0.05)。胡枝子叶片Pn与PAR、Ca、RH等生态因子间的拟合方程分别为:Y上pn=-26.148 0.002 3xPAR 0.11xCa-0.84xRH,Y平pn=-19.148 0.001 9xPAR 0.106xCa 0.86xRH,Y下pn=-27.148 0.002xPAR-1.16xRH,其主要生态影响因子及其大小顺序为XPAR>XRH>XCa。研究发现,不同坡位胡枝子叶片主要光合指标日变化趋势相似,但其峰值出现时间和高低有别,其主要生态影响因子为光合有效辐射、大气相对湿度和大气二氧化碳浓度。

Abstract:对青海省民和、西宁和湟源3个地区的药用植物薄荷(Mentha haplocalyx Briq.)的净光合速率和叶绿素荧光参数的变化进行分析比较。结果显示:(1)最大净光合速率(Pn)湟源>西宁>民和;(2)PSⅡ反应中心最大光化学效率(Fv/Fm)以及PSⅡ反应中心活性参数(1/Fo-1/Fm)和实际光化学量子效率(ΦPSⅡ)的变化趋势相同,均以西宁最高,而相应的光合功能的相对限制(LPDF)西宁最低;(3)西宁薄荷的光化学猝灭系数(qP)最高(0.875),而非光化学猝灭(NPQ)则最低(1.114)。研究表明,青海薄荷有光抑制现象发生,但并没有造成PSⅡ反应中心的不可逆破坏;西宁薄荷光化学能力较高而湟源薄荷对于过剩光能的耗散能力较高。

Abstract:The mycorrhizal structure type and species of arbuscular mycorrhiza fungi(AMF) in rhizosphere of 4 rare medicinal plants,Syringa reticulata var.amurensis,Cercidiphyllum japonicum,Taxus chinensis var.mairei and Euptelea pleiosperma in Lishan Nature Reserve

Abstract:Based on remote sensing images,spatial gradient variations of vegetation landscape heterogeneity was analyzed from east to west,north to south,and northwest to southeast in Jialing River basin by using software ArcView GIS and FRAGSTATS.The results showed

Abstract:Calligonum L.is an endemic and dominant plant in the desert areas of the central Asia.Which characters of drought-resistance,salt resistance and sand-loving,it is a good species for wind-break and sand-fix.The variability of characters of fruit is rich.Th

Abstract:The pteridophyte flora in Ruoliaoxian Nature Reserve are composed of 153 species(including varieties and subspecise),69 genera,38 families.Comparing with nine florae,the integratiye coefficient of this flora(0.161) is the fifth.The representative families

Abstract:This paper preliminarily investigated the floristic geographical features of the ferns in Emei Mountain.The results are as follows:there are 425 species belonging to 110 genera of 46 families,and the dominant families are Athyriaceae,Polypodiaceae and Dry

Abstract:In type specimens in Scrophulariaceae housed at the Herbarium(PE),Institute of Botany,the Chinese Academy of Sciences,the types of fourteen names of Chinese taxa are found to be syntypes under Article 9.4 and 37 Note 1 of the International Code of Botanic

Abstract:通过对Amana anhuiensis主模式的存放地中国科学院植物研究所标本馆（PE）的查找及向原作者证实,确认该种的模式已不存在。在对模式产地的野外调查过程中,发现仅有这一种老鸦瓣分布,且其花期及果实成熟期植株的一些重要形态性状与原描述不符或在原描述中没有提及,这些性状不仅在个体间比较稳定,而且在种的划分上具有重要价值。它们是：鳞茎皮黄褐色,薄纸质,内侧被绵毛;第1叶自基部向上3/4处最宽;苞片狭披针形;花被片内侧基部具黄绿色斑块;内轮3枚雄蕊比外轮3枚长,花粉黄色,雌蕊花柱与子房近等长。朔果近球形,果喙长10 mm左右。为此,我们指定了该种的新模式,并对其形态特征进行了补充描述。

Abstract:Roegneria zaquensis Y.H.Wu et S.L.Lu,a new species of Gramineae from Zadoi County,Qinghai Province,is described and illustrated.The new species is related to Roegneria yushuensis L.B.Cai.However,it has auricles on top at the both sides of sheath.The spike

Abstract:报道了前胡属（Peucedanum）1新变型——白花滨海前胡（Peucedanum japonicum f.album）,新变型与原变型的区别在于：花白色;而原变型花色为紫色。

Abstract:Lindera praecox f.pubescens of the Lauraceae,is reported as a new record to China and illustrated with a line drawing.This form differs from the typical one in having leaf costa sparsely pubescent on upper surface,costa and lateral veins densely pubescent

Abstract:Androsace erecta is reported as new record in Shaanxi.

Abstract:The genus Ceropegia Linn.(Asclepiadaceae) and the species Ceropegia paoshingensis Tsiang et P.T.Li are reported as new records of Shaanxi Province.

Abstract:Two newly recorded liverwort species from Qinling Mountain,Shaanxi,are reported,namely Cephaloziella rubella(Nees) Warnst.and Cephaloziella spinicaulis Douin..Based on the specimens,we elaborated the morphology of the two species and discussed their geogr

Abstract:14 species,subordinating to 14 genera and 12 families,are reported in this paper.Those newly reported species are found distributing in Zhongtiao Mountain area,Shanxi Province for the first time,which are Selaginella vaginata Spring,Leptolepidium kuhnii(M

Abstract:The fiber initiation,which decides the yield and quality of fiber directly,is the early stage of cotton fiber development.This paper reviews and discusses the recent achievement of cotton fiber differentiation and initiation through the temporal and spati

Abstract:Ⅰ型酪蛋白激酶(CKI)属丝氨酸/苏氨酸蛋白激酶,在真核生物中广泛存在,结构高度保守。在生物生长发育过程中CKI参与多种细胞功能及发育过程的调节,具有非常重要的作用。本文从CKI的结构、生理功能、调节机制以及近年来CKI在哺乳动物、酵母和植物中的分类与功能等方面进行综述,为进一步研究其在代谢及信号转导中的作用,明确其在生长发育等调控网络中的地位提供理论指导。

Abstract: