Abstract:Plant responses to abiotic stress are closely linked to the dynamics of microtubules, and the structure and function of tubulins are regulated by microtubule-associated proteins (MAPs). MAP70-1 is a microtubule binding protein, which plays a vital role in plant growth and development and stress. In order to reveal the molecular mechanism of MAP70-1 response to drought stress, we used the rootstock M26 of apple (Malus domestica Borkh.) as material, cloned the MdMAP70-1 gene (GenBank No. XM_008390949) by PCR and performed bioinformatics analysis. The ‘yellow pearl’ tomato was transformed by Agrobacterium-mediated leaf disc method and its drought resistance was analyzed. The results showed that: (1) MdMAP70-1 was successfully cloned and its open reading frame (ORF) was 1 860 bp and encoded 691 amino acids，the relative molecular weight was 68.77 kD and the isoelectric point (pI) 8.64. Comparison of amino acid sequences and phylogenetic analysis indicated that MdMAP70-1 contained a conserved domain of MAP70 family and had a closest relationship with VvMAP70-1 (Vitis vinifera VIT_201s0011g05790). (2) Wild tomato was transformed by Agrobacterium-mediated leaf disc method, 12 positive plants were obtained by resistance screening, and 8 overexpressed lines containing target bands (1 860 bp and 680 bp) were obtained by PCR, which proved that the MdMAP70-1 gene had been transferred into tomato. qRT-PCR detected and selected three transgenic lines OE-1, OE-2 and OE-3 with significantly higher expression levels than WT (wild type). (3) Analysis of drought stress resistance of T₃ generation transgenic tomatoes showed that there were no differences in the relative water content (RWC), proline (Pro) content, content of soluble protein (SP), malondialdehyde (MDA) content, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities of overexpressed tomato leaves under drought stress 0 and 5 days compared with control (WT). After 10 and 15 days of stress, compared with WT, the RWC, Pro content, SP content and antioxidant enzyme activities of the overexpressed MdMAP70-1 transgenic tomatoes were significantly higher than those of WT, while the relative conductivity (REC) and MDA contents were decreased. The study have shown that overexpression of MdMAP70-1 gene in tomato can increase the contents of RWC, Pro and SP, activities of SOD, POD and CAT in tomato, reduce the contents of REC and MDA, and enhance the drought resistance of tomato. The results of the study laid a foundation for the use of MdMAP70-1 gene to improve the drought tolerance of plants and the function of microtubule-associated proteins.

Abstract:In this study, ABI1 gene was cloned from Lilium oriental hybrid ‘Sorbonne’ by RT-PCR, and the bioinformatics analysis were performed. Meanwhile, the qRT-PCR was used to detect the expression patterns of LoABI1 in lily at different tissues and in the process of low temperature treatment and after transplanting, for clarifying the functional characteristics of LoABI1 gene and lay a foundation for the analysis of the ABA signal transduction pathway and the mechanism of breaking dormancy at low temperature in lily. The results showed that: (1) the LoABI1 gene of lily was cloned successfully. The coding sequence of LoABI1 was 1 341 bp long and LoABI1 encoded 446 amino acids containing a conserved phosphatase 2C (PP2C) domain. (2) Phylogenetic analysis showed that LoABI1 was closest to Oryza sativa and clustered with AtABI1, which is a member of Arabidopsis thaliana PP2C gene family, and both of them belong to PP2C A group. (3) The subcellular localization results showed that LoABI1 is localized in the nucleus and cytoplasm of tobacco epidermal cells. (4) Real time RT-PCR expression analysis showed that LoABI1 expressed in stem root, tender stem, leaf, and floral organ, and the expression level is higher in tender tissues; The expression of LoABI1 increased at first reaching the peak at the fifth week of cold storage, and then decreased during cold storage. Finally, it remained at a low-level during colonization. (5) The lily bulbs treated at 4 ℃ for 60 days could germinate at 14-28 days, and the buds could be seen at 28-48 days after transplanting, while the bulbs without low temperature treatment neither germinated nor flowered. It is speculated that LoABI1 gene may play an important role in the process of breaking bulbs dormancy at low temperature, and LoABI1 protein may play an important role in lily ABA signal transduction.

Abstract:In the present study, we cloned a DREB gene by PCR based on the third-generation transcriptomic data of Psammochloa villosa and analyzed by bioinformatics. We analyzed the expression pattern of PvDREB gene and its expression characteristics under the drought stress of 20% PEG-6000 through real-time quantitative PCR to explore the function and role of DREB transcription factor under drought stress. It lays a foundation for revealing the molecular mechanism of PvDREB gene of P. villosa response to drought stress. The results showed that: (1) we have successfully cloned a DREB gene named PvDREB. The coding sequence length of PvDREB gene was 831 bp, which encoding 276 amino acids and containing a typical conserved domain of AP2 transcription factor. It was a stable hydrophobic protein without signal peptide structure, and had possible transmembrane domain, glycosylation, and phosphorylation sites. (2) The result of phylogenetic analysis indicated that PvDREB gene of P. villosa had the closer relationship with DREB gene of Phyllostachys edulis. (3) The prediction of subcellular localization showed that PvDREB protein was localized in mitochondria and nucleus. (4) qRT-PCR analysis suggested that PvDREB could be induced and expressed in the roots, stems and leaves of P. villosa, but the difference was large, and the expression level was the highest in stems, followed by leaves, and the lowest in roots, with obvious tissue specificity. Under 20% PEG-6000 simulated drought, the expression of PvDREB gene in leaves increased with the increase of drought stress time, reached the highest at 12 h, and then decreased gradually. It was speculated that the expression of PvDREB gene was induced by drought stress, and this gene may play an important role in the response of P. villosa to drought stress.

Abstract:In order to investigate the function of PoKAS gene in the fatty acid synthesis of P. ostii ‘Feng Dan’, we obtained three PoKAS genes from the transcriptome data, cloned the genes and conducted the bioinformatics analysis. Their expressions were examined by qRT-PCR at 23, 45, 75, 100 and 125 days after flowering. The results showed that: (1) the full sequence length three cloned genes were 1 401, 1 692 and 1 215 bp, encoding 466, 563 and 404 aa, respectively. The analysis of the conserved domains revealed that they all contained the conserved domain of KAS and belonged to the superprotein family of cond-enzymes. (2) The phylogenetic tree analysis showed that they were clearly divided into three categories, indicating that they were relatively evolutionarily independent, named PoKASⅠ, PoKASⅡ and PoKASⅢ (GenBank accession numbers OP056413, OP056412, and OP056414, respectively). (3) The qRT-PCR analysis revealed, among the five periods of peony seed development after the flower drop, the expressions of PoKASⅠ and PoKASⅡ were significantly higher at 75 d and 45 d after flowering than those at other development periods; the expression of PoKASⅢ wassignificantly higher at 45-125 d after anthesis than that at 23 d after anthesis, indicating that PoKASⅢ could play an important role in the whole process of fatty acid synthesis in peony seeds, while PoKASⅡ mainly could play a role in the early stage of rapid accumulation of seed oil. It is hypothesized that this gene family from P. ostiis ‘Feng Dan’ could play different roles in different developmental periods during the formation of seed fatty acids.

Abstract:Using Mikania micrantha cutted and planted in Mengxiu forest farm of Ruili City, Yunnan Province as test materials, we quantitatively analyzed the contents of indole-3-acetic acid (IAA), gibberellins (GAs), abscisic acid (ABA), trans-zeatin (tZ), N6-Isopentenyladenine (IP), 1-aminocyclopropanecarboxylic acid (ACC), jasmonic acid (JA) and salicylic acid (SA) in flower buds at the undifferentiated stage of flower buds and the differentiation stage of inflorescence primordium by liquid chromatography mass spectrometry (LC-MS/MS). Based on the transcriptome gene function annotation data, we also analyzed the expression of genes related to endogenous hormone synthesis, metabolism and signal transduction pathways. The aim of this study was to explore the regulatory effects of different endogenous hormones on flower bud formation of M. micrantha, and analyze the mechanism of endogenous hormone synthesis and signal transduction pathway related genes regulating flower bud differentiation of M. micrantha. It provides theoretical and technical support for controlling the sexual reproduction of M. micrantha by regulating the endogenous hormone level of M. micrantha with exogenous hormones in the later stage. The results showed that: (1) the contents of GA₁₅, GA₁₉, GA₂₀, GA₂₄, IAA, ABA and ACC in flower bud at undifferentiated stage were lower than those at inflorescence primordium differentiation stage, while the contents of tZ and IP in flower bud at undifferentiated stage were significantly higher than those at inflorescence primordium differentiation stage. (2) Based on RNA-seq results, a total of 7 116 differentially expressed genes (DEGs) were obtained at two flower bud differentiation stages of M. micrantha, of which 3 907 were up-regulated and 3 209 were down regulated. (3) In terms of endogenous hormone synthesis, a large number of DEGs involved in the synthesis of GA₁₅, GA₁₉, GA₂₀, GA₂₄, IAA, ABA and ACC were up-regulated during inflorescence primordium differentiation, which was consistent with their high content trend during inflorescence primordium differentiation of M. micrantha. The high expression of YUUCA gene family involved in IAA synthesis and ACS gene involved in ETH at inflorescence primordium differentiation stage may also be involved in promoting flower bud differentiation of M. micrantha. (4) In the plant hormone transduction pathways, flower bud differentiation of M. micrantha was regulated by down-regulation of AUX/IAA gene (gene-E3N88_07743) and up-regulation of ARF (gene-E3N88_41119) in auxin signal transduction pathway, up-regulation of ERF (gene-E3N88_41547) in ethylene signal transduction pathway, up-regulation of GID1 (gene-E3N88_19448) in gibberellin signal transduction pathway, down-regulation by B-ARR (Gene-E3N88_28086) and A-RRR (gene-E3N88_40764) in cytokinin signal transduction pathway, up-regulation of AREB (gene-E3N88_18558) in abscisic acid signal transduction pathway, up-regulation of JAZ (gene-E3N88_05628) and down-regulation of MYC2 (gene-E3N88_32405) in jasmonic acid signal transduction pathway. Therefore, high levels of GA₁₅, GA₁₉, GA₂₀, GA₂₄, IAA, ABA and ETH are conducive to flower bud differentiation of M. micrantha. The flower bud differentiation process of M. micrantha regulates the hormone concentration by changing the expression of different kinds of endogenous hormone synthesis and metabolism genes, and the hormone causes the expression of downstream genes through signal transduction pathway to regulate the flower bud differentiation of M. micrantha.

Abstract:Rare and endangered M.oleifera seeds dried in shade after peel removal are used as materials, stratification treatment in greenhouse sand pond, different germination stages from stratification treatment to cotyledon excavation, we investigated the change characteristics of seed development morphology, storage material accumulation, enzyme activity and seedling type of M.oleifera, to understand the causes of seed dormancy. The results showed that: (1) it takes about 195 days for M. oleifera seed to germinate and form seedlings from the beginning of dormancy release. It takes about 75 days for the morphological development of young embryo to ripen, and then 30 days later is the period when the seeds germinate intensively, with the highest germination rate. This process can be divided into 7 stages (S1-S7 stages) according to the characteristics of the developmental morphology of the embryo: The seeds do not germinate at S1 stage; S2 stage seed embryo “exposed”; At S3 stage, the radicle breaks through the seed coat more than 1 cm long; At S4 stage, the junction between hypocotyl and radicle forms a hooked structure; The formation of “S” type embryo and the enlargement of the front end of radicle at S5 stage; At S6 stage, the seeds not only have enlarged radicles, but also have lateral roots; At S7 stage, cotyledons fall off, germ unearthed and true leaves appear. (2) M.oleifera seeds in the process of wet sand stratification, seed embryos length and embryo rate increased from (5.49±1.57) mm and (19.48±5.72)% during stratification at S1 stage to (67.92±2.94) mm and (240.75±15.29)% at S6 stage, the embryo rate increased by 12.4 times on average. It shows that the embryo of M. oleifera seeds needs to go through the post-ripening process to germinate, which belongs to the post-embryonic type seeds. (3) From the perspective of seed physiological indicators, with the increase of wet sand stratification time, soluble protein content and starch content in seed embryo decreased first and then increased, while the contents of soluble sugar and malondialdehyde (MDA), and the activities of superoxide dismutase (SOD) and peroxidase (POD) increased first and then decreased; However, the size and shape of endosperm did not change significantly at the same time, the contents of starch, soluble sugar, soluble protein, and the activities of SOD and POD showed a general downward trend. (4) The percentage of embryo and germination of seeds were negatively correlated with the contents of soluble protein and starch in endosperm, there was a very significant positive correlation with soluble sugar content (P<0.01). The study found that, M. oleifera seed undergoes a series of physiological and biochemical changes from dormancy release to germination, degradation and enzymatic activity of macromolecules including soluble proteins in the endosperm where nutrients are stored. It provides material and energy for the completion of post-ripening of seed embryos, releasing dormancy and entering germination. The physiological change process and the morphological development process of seed embryos cooperate with each other to complete the germination of M. oleifera seeds. Seedlings have completed germination to form original cotyledon-shaped seedlings, the cotyledons only have the function of absorbing nutrients but not assimilating.

Abstract:The main grape varieties ‘Red Globe’, ‘Flame Seedless’, ‘Hutai No.8’ and ‘Summer Black’ were used as test materials. We set up Open-filed cultivation (OFc) and Shelter-rain cultivation (SRc) pattern, to analyze the two kinds of table grapes from leaf photosynthetic parameters, physiological indexes, disease resistance and shoot growth. The results showed that: (1) the net photosynthetic rate (P_n) and transpiration rate (T_r) of table grape leaves under SRc were lower than those under OFc, and the stomatal conductance (G_s) of leaves under SRc was higher than OFc. SRc significantly promoted the increase of chlorophyll a, chlorophyll b and total chlorophyll contents in table grape leaves, and decreased the ratio of chlorophyll a/b, and the effect of SRc was more significant in ‘Red Earth’ and ‘Flame Seedless’. (2) There was no significant difference in the contents of total soluble sugar and soluble protein in grape leaves between SRc and OFc modes; The SA (salicylic acid) content in the leaves of table grape under OFc was significantly higher than that under the SRc, and the increase of SA content in the leaves of downy mildew resistant grape varieties ‘Hutai No.8’ and ‘Summer Black’ was significantly higher than that susceptible grape varieties ‘Red Globe’ and ‘Flame Seedless’. (3) In conclusion, SRc can effectively control the occurrence of table grape diseases; SRc greatly reduced the rate of diseased leaves, reduced the disease index of leaves, and increased the grade of resistance to disease of grapes, especially in Vitis vinifera L. (4) Compared with OFc, the length and thickness of table grape branches increased significantly under SRc, and ‘Hutai No.8’ and ‘Summer Black’ showed extremely significant increase after grape fruit harvesting. Therefore shelter-rain cultivation significantly reduced the occurreue of table grape diseases, improvcd leaf photosynthesis efficiency, it is beneficial to promote the growth and development of table grapes in the current year, is the main way to cultivate high-quality grapes.

Abstract:In order to explore the differences of gender response of Hippophae rhamnoides subsp. sinensis to cadmium (Cd) in soil, this study used biennial seedlings of H. rhamnoides subsp. sinensis as materials to study the differences in growth, leaf physiological characteristics and Cd accumulation characteristics of female and male seedlings under Cd concentration of 0(CK), 25, 50, 100 and 200 mg·kg^-1. The results showed that: (1) under Cd treatment, the growth of plant height, basal diameter and biomass of female and male H. rhamnoides subsp. sinensis seedlings showed the phenomenon of low concentration (< 50 mg·kg^-1) promotion and high concentration (> 100 mg·kg^-1) inhibition. Under low concentration of Cd treatment, the growth rate of plant height, basal diameter and biomass of female seedlings were higher than those of males. Under high concentration of Cd treatment (200 mg·kg^-1), the growth of plant height, the leaf biomass and the total biomass of male seedlings were significantly lower than those of CK, while those of female seedlings were not decrease significantly. (2) With the increasing of Cd concentration, the content of photosynthetic pigments and the activities of antioxidant enzymes in the leaves of female and male seedlings increased at first and then decreased, and the contents of malondialdehyde (MDA) and osmoregulation substances increased. When the Cd concentration was 50-200 mg·kg^-1, the photosynthetic pigment content, antioxidant enzyme activities and osmoregulation substance contents in leaves of female seedlings were higher than those of males, while the MDA content was always lower than that of the males. (3) With the increasing of Cd concentration, the Cd content in various organs of female and male seedlings gradually increased and showed a trend of root > stem > leaf. The Cd content in various organs of female seedlings was higher than that of male seedlings. The bioconcentration factors (BCF) of aboveground and underground parts of female and male seedlings increased at first and then decreased, and all of them were higher than 1, while the translocation factors (TF) gradually decreased and were less than 1. The BCF and TF of aboveground and underground parts of female seedlings were higher than those of male seedlings. When the Cd concentration was 25-100 mg·kg^-1, the BCF of aboveground parts was significantly higher than that of male seedlings. It was found that H. rhamnoides subsp. sinensis seedlings showed tolerance to Cd in certainly degree when the Cd concentration in soil was less than 50 mg·kg^-1. When the Cd concentration in soil was higher than 100 mg·kg^-1, the tolerance to Cd was weakened. The growth adaptability, physiological tolerance, enrichment and transport capacity of female seedlings to Cd in soil were stronger than male seedlings.

Abstract:Preharvest sprouting (PHS) could cause serious damage to grain yield and quality of quinoa, and the abscisic acid (ABA) is closely related to seed germination and seed dormancy. In this study two samples susceptible to PHS such as BBQ3 and WQ6 and two resources unsusceptible to PHS namely SBQ1 and WQ4 were used as experimental materials. Within 3 days of seed germination, they were applied with different concentrations of ABA and fluoridone (FL) to explore its impacts on the germination of two-color quinoa seeds, ABA content and the expression of ABA synthesis gene NCED, lysis gene 8′OH and signal transduction gene ABI3. The results showed as follows: (1) the application of different exogenous ABA concentrations had inhibitory effects on the seed germination of all quinoa materials, and the 25 μmol·L^-1 concentration had the best effect. Low exogenous FL concentration had a promotion effect on the seed germination of all quinoa materials and the 2.5 μmol·L^-1 concentration was the best. After the ABA and FL were applied, the germination rate of SBQ1 and WQ4 decreased and increased respectively less than BBQ3 and WQ6. (2) The endogenous ABA content of SBQ1 and WQ4 seeds in the control group was significantly higher than that of BBQ3 and WQ6 seeds, which was consistent with the results of PHS resistance and seed germination. The treatment of exogenous ABA could significantly increase the endogenous ABA content of quinoa seeds, and the ABA content of WQ4 seeds was significantly higher than that of WQ6 seeds, the endogenous ABA content of SBQ1 with slightly thicker pericarp was significantly lower than that of BBQ3. The endogenous ABA content in seeds of the four materials decreased after exogenous FL treatment, only SBQ1 reached a significant level, which was consistent with the results of seed germination. (3) In the control group, the expression levels of NCED, 8′OH and ABI3 genes of SBQ1 and WQ4 unsusceptible to PHS was respectively higher than those of BBQ3 and WQ6 susceptible to PHS. After exogenous ABA treatment, the expression levels of NCED and ABI3 genes of SBQ1 were significantly up-regulated at 12 h, the expression levels of the three genes of BBQ3 were significantly up-regulated at 6 h, and the expression levels of the three genes of WQ4 were also up-regulated later than that of WQ6. Under exogenous FL treatment, the expression levels of three genes of SBQ1 were all up-regulated, but the expression levels of NCED were significantly lower than that of 8′OH, the expression levels of NCED and ABI3 of BBQ3 were down-regulated, and the expression levels of 8′OH were significantly up-regulated. FL mainly inhibited the expression of NCED and 8′OH of WQ4, but had no significant regulatory effect on ABI3, while the promotion effect of FL on 8′OH gene expression was higher than that on NCED gene in WQ6. (4) The results of correlation analysis showed that seed germination rate and ABA content were significantly correlated with the expression levels of NCED and ABI3 genes in black quinoa and white quinoa. The results showed that the germination rate of quinoa unsusceptible to PHS was lower than that of materials susceptible to PHS, and the ABA content was higher than that of materials susceptible to PHS. Exogenous ABA could significantly inhibit seed germination, and the sensitivity of materials susceptible to PHS to ABA was higher than that of materials unsusceptible to PHS, and the expression of related genes in materials with high ABA sensitivity showed earlier response to ABA than other materials. FL can promote seed germination, but it may not directly regulate ABA synthesis by inhibiting NCED, ABI3 expression and promoting 8′OH expression, but by affecting other key binding sites.

Abstract:In order to explore the distribution of Carex L. plants in different vegetation types, this study investigated the community characteristics of 9 Carex L. plant species in central and eastern Gansu Province, and compared and analyzed the soil nutrient status, to reveal the relationship between the species diversity and distribution communities and soil environmental factors of wild Carex L. The results show that: (1) the species diversity of the 9 Carex communities was significantly different. The Shannon-Wiener diversity index (H) and Simpson dominance index (D_si) were the highest in Carex breviculmis community, and the lowest in the Carex lanceolata community. The Patrick richness index (R) was the highest in Carex heterostachya community and the lowest in Carex rigescens community. The Pielou evenness index (J_sw) was the highest in Carex melanostachya community and the lowest in C. lanceolata community. (2). The soil suitable for the growth of 9 wild Carex species was neutral or weakly alkaline, and the contents of organic matter, nitrogen and potassium were rich, but the content of phosphorus was low. The average contents of soil organic matter, pH, total nitrogen, total phosphorus, total potassium, available nitrogen, available phosphorus, and available potassium were 41.07 g·kg^-1, 8.35, 1.16 g·kg^-1, 0.65 g·kg^-1, 5.60 g·kg^-1, 47.94 mg·kg^-1, 5.82 mg·kg^-1and 100.60 mg·kg^-1, respectively. (3) The species diversity of 9 Carex species was positively correlated with total nitrogen, total phosphorus, total potassium, alkali-hydrolyzed nitrogen, organic matter, rainfall and elevation, but it was negatively correlated with soil pH, available phosphorus and available potassium. Rainfall, soil pH, available phosphorus and organic matter had great effects on the community species diversity of 9 wild Carex species.

Abstract:The Karst peak-cluster landform forest in Maolan is a rare non-zonal forest ecosystem with strong originality and less human disturbance, which is quite different from the normal landform zonal forest ecosystem. In this study, the typical sample plot survey method was used to 11 vertical transect plots in the Maolan Karst forest with an altitude of 620-910 m. We analyzed the vertical gradient variation characteristics of the species richness of the top community in the study area, and used redundancy analysis (RDA) method to analyze the effects of environmental factors on species richness, so as to clarify the relationship between species richness and environmental factors on the vertical gradient, and provide a theoretical basis for the maintenance and restoration, protection and management of Karst natural forest ecosystem. The results showed that: (1) a total of 511 species of vascular plants belonging to 124 families and 272 genera were recorded in the Maolan karst peak cluster. (2) One-way ANOVA analysis showed that the richness of different life forms in the community was very significantly different (P<0.01); Multiple comparison analysis of LSD showed that the richness level of community was overall > shrub layer > herb layer > tree layer. (3) With the increase of altitude gradient, the total species richness of community and the species richness of herb layer were significantly negatively correlated with altitude (P<0.05), while the tree layer richness and shrub layer richness were no obvious regularity with altitude. (4) Redundancy analysis showed that the contribution rate of soil C/N/P reached 58%, which was the main factor affecting species richness.

Abstract:In this study, bryophyte investigation and specimen collection were carried out on the north slope of Dabie Mountain in Anhui Province for four times from 2019 to 2021. Based on the identification of 1 517 specimens collected and published literatures, the bryophyte composition and floristic composition in this area were studied. At the same time, the attribution of bryophyte flora on the northern slope of Mt. Dabie were explored through comparison with bryoflora of east, north and central China. The results showed that: (1) a total of 482 species (including infraspecific taxa) of bryophytes belonging to 180 genera and 80 families were in the study area, and 206 species, 40 genera and 12 families were added to the survey, among which 9 species were new additions to Anhui Province. (2) Two threatened bryophytes were found, Glyphomitrium minutissimum belonging to CR and Bryum bornholmense belonging to VU. (3) There were three worldwide monotypic families, 14 monotypic genera and 34 oligotypic genera (2-6 species), accounting for 26.67% of the total genera, which reflected the ancient and primitive nature of the bryophyte flora in the area. (4) The bryophyte flora in this area was complex and diverse, of which North Temperate components (26.43%) and East Asia components (24.89%) were predominant, followed by Tropical Asia components (14.54%). (5) The bryophyte flora of this area was most closely related to the flora of east China (The western Tianmu Mountain, Qingliangfeng National Reserve and Yangjifeng National Reserve), because of its similar geoclimatic characteristics and species composition. Show that, the bryophyte flora of the northern slope of Mt. Dabie should belong to the flora of east China.

Abstract:Zingiber atroporphyreum korniｃ_ˇk. & Q.B.Nguyê_~n, discovered in Malipo county of Yunnan, is reported as a newly recorded species of Zingiber (Zingiberaceae) to China. Morphological characteristic description, color photos and distribution information are provided. Voucher specimens were deposited in herbarium of East China Normal University (HSNU).

Abstract:Southern Tibet has a more complex topography, rich plant resources and high species diversity. For the survey of alpine plant diversity in the Shannan region of Tibet, field surveys, specimen collection, review of botanical histories and other relevant information and taxonomic identification were conducted. The results indicate that Arenaria globiflora (Fenzl) Edgew. & Hook. f. and Silene birgittae Bocquet are newly recorded species in China. This report, on the one hand, enriches the flora of China, updates the diversity of domestic Caryophyllaceae family, and has some significance for the conservation and study of plant diversity in the Shannan region of Tibet, China. On the other hand, it shows that plant diversity surveys in some areas of Shannan, Tibet, China are still inadequate and there are still many survey weak areas, which need to strengthen plant diversity surveys.