WANG Zheng , WANG Gaiping , ZHAO Qun , ZHAI Jinting , YU Pengfei
2024, 44(11):1673-1681. DOI: 10.7606/j.issn.1000-4025.20240330
Abstract:[Objective] To investigate the effects of different light qualities on the growth, photosynthesis, flavonol accumulation and antioxidant capacity of Ginkgo biloba, and to screen out the more suitable light qualities for the growth and flavonol accumulation of Ginkgo biloba, so as to provide theoretical basis for the improvement of Ginkgo biloba"s medicinal value. [Methods] Two-year-old Ginkgo biloba seedlings were used as test materials, and four light quality treatments with red and blue ratios of 1 : 1 ( 1R1B ), 1 : 3 ( 1R3B ), 1 : 5 ( 1R5B ) and white light ( W, control ) were set up. After 20 days of treatment, the growth, photosynthetic physiology, flavonol content and antioxidant capacity of Ginkgo biloba were analyzed. [Results] Different ratios of red and blue light reduced Ginkgo biloba biomass and were detrimental to plant height and ground diameter compared to white light. 1R5B treatment could promote the increase of leaf area, total chlorophyll content and Ci. The Tr and Gs of leaves and the total biomass of Ginkgo biloba were increased by 1R1B treatment. The contents of Pn, Fm, Fv / Fm, Fv / Fo, qP, carotenoids, total flavonols and antioxidant capacity of leaves under 1R3B treatment were the highest. [Conclusion] Different proportions of red and blue light had an effect on the growth and photosynthetic characteristics of Ginkgo biloba, and significantly increased the content of flavonols and antioxidant capacity. Comprehensive analysis showed that 1R3B treatment was most conducive to the accumulation of flavonoids in Ginkgo biloba, which was the ideal light quality.
CAI Juan , ZHAO Lijun , ZHU Liqiong , JIN Yun , ZHAO Yuanyuan
2024, 44(11):1682-1691. DOI: 10.7606/j.issn.1000-4025.20240292
Abstract:[Objective] The study aims to explore the growth and physiological response characteristics of Canavalia maritima seedlings under water stress, and provide a reference for water management during the seedling stage and for the cultivation of high-quality plants in coastal sandy lands.[Methods] One-year-old C. maritima seedlings were subjected to simulated drought (soil relative water content at 20%, 40%, and 60%), suitable water content (80%, control), and waterlogging (100%) through a pot water control experiment. Their growth and physiological indices were analyzed. [Results] Under both drought and waterlogging stress, leaf dry weight significantly decreased, while leaf relative water content significantly increased. Seedling height and leaf area significantly increased under waterlogging but significantly decreased under severe drought. Total root length, total root surface area, total root volume, and mean root diameter increased under drought and decreased under waterlogging. The content of malondialdehyde and the activities of catalase (CAT) and peroxidase (POD) significantly increased, while the activity of superoxide dismutase (SOD) remained relatively stable. Soluble protein content decreased, whereas soluble sugar and proline contents significantly increased. The chlorophyll content significantly decreased under severe drought. Initial fluorescence significantly increased, while maximum fluorescence , maximum photochemical efficiency, and maximum quantum yield significantly decreased.[Conclusion] Under drought and waterlogging stress, C. maritima seedlings demonstrated strong tolerance by altering water accumulation and distribution patterns, increasing the activities of CAT and POD, and enhancing soluble sugar and proline contents.
SHI Wenxin , LI Ai , ZHANG Weihua , ZHANG Liangge , LANG Junkai , XU Lingxing
2024, 44(11):1692-1702. DOI: 10.7606/j.issn.1000-4025.20240429
Abstract:[Objective] The purpose of this study was to investigate the mitigation effect and physiological mechanism of different concentrations of salicylic acid (SA) on high temperature stress injury of watermelon seedlings, and to provide a theoretical basis for the rational application of SA in watermelon cultivation and production. [Methods] Potted ‘K53’ watermelon seedlings in greenhouse were used as experimental materials. After spraying different concentrations (0,0.5,1.0,1.5,2.0 mmol/L) of SA on the leaves, the seedlings were stressed at 42°C for 36 h day and night. The growth characteristics, anatomical structure, physiological and biochemical indexes of watermelon seedlings were observed, and the mitigation effects of each treatment were comprehensively evaluated by membership function method. [Results] After high temperature stress, the leaves of watermelon seedlings wilted seriously, the growth of root length was inhibited, the leaves became thinner, and the mesophyll cell structure was damaged. After SA application, the leaves became thicker, photosynthetic pigment content, photosynthetic gas exchange parameters, antioxidant enzyme (POD, SOD, CAT) activity and osmotic adjustment substances (proline, soluble sugar, soluble protein) content increased significantly, MDA content and relative conductivity decreased significantly. Compared with the high temperature stress treatment group, the MDA content of leaves under 1.0 mmol/L SA treatment was significantly reduced by 33.70%, the relative conductivity was significantly reduced by 58.58%, the contents of chlorophyll a and chlorophyll b were significantly increased by 22.71% and 21.63%, respectively. The net photosynthetic rate was significantly increased by 98.34 %, and the activities of SOD and CAT were significantly increased by 23.98% and 19.71%, respectively. [Conclusion] Pre-spraying different concentrations of SA could alleviate the damage of watermelon seedlings under high temperature stress, and 1.0 mmol/L SA treatment had the best effect.
XIAO Mengna , HU Yuehao , LAN Zengquan , LUO Yiju , TAO Yanlan , Wang Dexin
2024, 44(11):1703-1713. DOI: 10.7606/j.issn.1000-4025.20240373
Abstract:【Objective】 To explore the changes in photosynthetic physiology and leaf structure of tea trees under different intercropping patterns. 【Methods】Four intercropping modes, namely, R delavayi-tea(MT), winter cherry-tea (DT), walnut-tea (HT), and pure tea plantation (CK), within the planting range of the Cherry Blossom Valley of Mount Wuliang in Nanjian County, Yunnan Province, were investigated through the study of leaf anatomy of tea trees and the changing law of the tea tree"s photosynthetic physiological and ecological functions under different intercropping modes. 【Results】The three intercropping modes significantly reduced the photosynthetically active radiation flux (PAR) and the temperature of tea tree leaves (TL) in the spring, and the net photosynthetic rate (Pn), and transpiration rate (Tr) of different intercropping modes in the spring and autumn roughly showed a single-peak curve with a rise followed by a decline, in which the CK mode differed significantly from the other intercropping modes, and the daily change of the intercellular carbon dioxide concentration (Ci) showed a U-shaped curve, with the first decline and then increase. The thickness of fenestrated tissue and leaf thickness of tea leaves in different intercropping modes in spring and autumn were significantly lower than that in CK mode; PAR was significantly positively correlated with TPT, P/S and CTR in spring and negatively correlated with CLR in autumn, and Pn was significantly positively correlated with CTR in autumn. 【Conclusion】Different intercropping modes effectively affected the photosynthetically active radiation flux, leaf temperature, net photosynthetic rate, transpiration rate, stomatal conductance, and intercellular carbon dioxide concentration of tea trees in tea plantations, among which the effects were more significant in the two intercropping modes of MT and DT. Tea trees grown under different intercropping modes will gradually change leaf morphology to adapt to different environmental characteristics; there is a certain correlation between the anatomical structure of tea tree leaves and photosynthetic physiology, and the thickness of palisade tissues, lower epidermal thickness, ratio of palisade to sponge tissue ,and the cell tense ratio can be used as an important index for determining the photosynthetic capacity of the leaves in a certain period of time and within a certain range.
ZHANG Rongrong , WANG Yanzhi , WANG Zitin , ZHANG Baolin
2024, 44(11):1714-1724. DOI: 10.7606/j.issn.1000-4025.20240372
Abstract:【Objective】 The study aims to investigate the physiological effects of zinc oxide nanoparticles (ZnO NPs) on the "Ningqi No. 10" wolfberry seedlings under saline-alkali stress, evaluate its physiological mechanism for alleviating saline-alkali stress, and explore its application potential and suitable concentration. 【Methods】 One-year-old "Ningqi 10" wolfberry potted seedlings were used as materials. They were first irrigated with ZnO NPs solutions of different concentrations (50, 100, and 150 mg/L) at the root, followed by 100 mmol/L saline-alkali stress treatment. Samples were taken after 15 and 30 days of stress to measure seedling biomass, photosynthetic pigments, osmoregulatory substances, Na+, K+, reactive oxygen species (ROS) content, antioxidant enzyme activity, and AsA-GSH cycle-related indicators. A comprehensive evaluation of the treatment effects was conducted. 【Results】 ZnO NPs pretreatment significantly promoted the accumulation of biomass in wolfberry seedlings under saline-alkali stress, increased leaf chlorophyll content, enhanced the content of antioxidant substances and related enzyme activities in the AsA-GSH cycle, as well as SOD, POD, and CAT activities. It significantly reduced cell membrane damage and lipid peroxidation, significantly increased the accumulation of soluble sugar, proline, and soluble protein, and regulated the Na+/K+ balance. Among the tested concentrations, 100 mg/L ZnO NPs treatment showed the best mitigation effect. 【Conclusion】 Root application of ZnO NPs effectively alleviated the growth inhibition of wolfberry seedlings under saline-alkali stress by promoting chlorophyll accumulation, regulating the AsA-GSH cycle and antioxidant enzyme activity to efficiently scavenge excessive ROS, enhancing osmotic adjustment capacity, and maintaining Na+/K+ balance.
CHEN Yi , YANG Ni , LUO Wei , LI Jingwen , LIU Hui , ZHUANG Jing
2024, 44(11):1725-1734. DOI: 10.7606/j.issn.1000-4025.20240303
Abstract:【Objective】 The identification and characterization of the BRX family genes in tea plant, along with the analysis of their expression patterns in various tissues and under stress conditions. The research lays the groundwork for investigation into the functions of the tea plant BRX genes. 【Methods】 Employed the tea plant genome database and bioinformatics software such as TBtools, we have conducted an identification and analysis of BRX genes. The experimental material was tea plant variety ‘Shuchazao’. Different treatments including salt stress, drought, high temperature, low temperature, and exogenous GA3 application have been applied to it for various durations. The relative transcript abundance of the BRX gene family across different tissues and under these stresses conditions was quantified using RT-qPCR. 【Results】 A total of 5 CsBRX genes have been identified, encoding proteins composed of 313~370 amino acids. The structures of genes were similar and highly conserved. The promoter regions contained hormone and stress response elements. Based on evolutionary relationships, BRX proteins were divided into 4 subfamilies. CsBRX1, CsBRX3 and CsBRX5 were all expressed in young leaves, while CsBRX4 was exclusively expressed in old leaves. Under salt stress for 12 h, the transcriptional levels of CsBRX1, CsBRX2 and CsBRX4 were elevated to the greatest extend. CsBRX2 exhibited a swift response to low-temperature stress, with its relative expression peaked at the 1 h mark. CsBRX2 demonstrated a positive response to exogenous GA3 signaling, and its expression reached a zenith at the 24 h interval. 【Conclusion】 The CsBRX genes demonstrated tissue-specific expression patterns, responded variably to multiple stresses and GA signal, which offered insights for the investigation of stress tolerance mechanisms within tea plants.
HU Yaofang , LI Ao , YIN Jiahui , WANG Yuancheng , LIU Junxiang , SUN Zhenyuan
2024, 44(11):1735-1742. DOI: 10.7606/j.issn.1000-4025.20240306
Abstract:【Objective】 ABA is a key hormone for drought stress in plants. Differential metabolites and their associated pathways of ABA-mediated dehydrated response in the isolated branches of Salix matsudana were revealed by metabolomics analysis, which provided the research basis and the theoretical basis for the response to drought stress in S. matsudana. 【Methods】The authors investigated the differential response of the xylem metabolomes of the S. matsudana branches dehydrated to the different water potentials after soaked in the sodium tungstate solution, an inhibitor of ABA synthesis, using the isolated branches of S. matsudana as materials. 【Results】 Compared with the deionized water-soaked non-dehydrated branches, the dehydrated to P50 water potential branches: The abscisic acid content was significantly increased by 4.25 times, and the content of the soluble sugars such as D-fructose was significantly increased along with the content of amino acids such as histidine. ABA synthesis inhibitor-soaked branches compared to deionized water-soaked branches: The content of abscisic acid was significantly reduced by 3.82 times, and the content of the soluble sugars such as D-1,5-defructose and amino acids such as phenylalanine was significantly reduced after soaked in the inhibitor of ABA synthesis. Plant hormone signal transduction, carotenoid biosynthesis and starch and sucrose metabolism pathways were the key pathways in the dehydrated response of S. matsudana branches. 【Conclusion】 In the S. matsudana branches after dehydration, the ABA content increased significantly, which mediated the starch hydrolysis process and other processes to reduce the osmotic potential of cells in response to the drought stress.
CAI Zhaoming , CHENG Chunhong , YU Hong , WANG Diandong
2024, 44(11):1743-1751. DOI: 10.7606/j.issn.1000-4025.20240286
Abstract:[Objective] Tuber mustard has high economic value. Investigating the function of jasmonic acid signaling pathway gene JAZ8 in plant response to salt stress can provide genetic resources for breeding resistant varieties of tuber mustard. [Methods] The gene structure, promoter cis-acting elements and the physical and chemical properties of JAZ8 were analyzed by bioinformatics method. JAZ8 gene was cloned by molecular biology and overexpression vector was constructed. The JAZ8 gene of tuber mustard was overexpressed in the model plant Arabidopsis Thaliana by transgenic technology and the specific function of JAZ8 gene in Arabidopsis thaliana in response to salt stress was analyzed. [Results] The JAZ8 gene coding sequence was 396 bp, encoding 131 amino acids, containing TIFY and CCT-2 domains. The gene promoter contains cis-acting elements that respond to abscisic acid, salicylic acid and participate in defense and stress response. The phenotypic analysis of transgenic plants showed that Arabidopsis plants overexpressing BjuJAZ8 showed a more sensitive phenotype to salt stress during germination and greening. [Conclusion] Overexpression of JAZ8 in tuber mustard enhanced the sensitivity of Arabidopsis to salt stress.
LIU Tongjin , LIU Zhenyang , BAN Qiuyan , ZHOU Lu , CUI Qunxiang
2024, 44(11):1752-1759. DOI: 10.7606/j.issn.1000-4025.20240327
Abstract:【Objective】TIFY transcription factors are involved in the regulation of growth and development processes in plants. This experiment involves the identification of the members of the TIFY family in Luffa cylindrica, selecting the highly expressed members in the fruit, and further cloning and investigating their expression patterns in different tissues, thus providing a basis for the functional identification of these genes. 【Methods】Bioinformatics methods were used to identify members of the TIFY gene family in L. cylindrica. Transcriptome analysis was performed to screen for highly expressed members in L. cylindrica fruit, followed by cloning using RT-PCR. Tissue expression was analyzed using qRT-PCR. 【Results】 The L. cylindrica genome contains 17 TIFY genes, unevenly distributed across 9 chromosomes, with CDS lengths ranging from 297 bp to 1 353 bp, classified into four subfamilies. Transcriptome analysis revealed that LcTIFY2, LcTIFY7, LcTIFY11, and LcTIFY13 are significantly more highly expressed in L. cylindrica fruit compared to other members. The CDS sequences of these genes were cloned, with structures comprising 3, 5, 6, and 2 exons, respectively. Tissue expression analysis indicated that all four genes have the highest expression in the peel of L. cylindrica fruit. Additionally, LcTIFY7 and LcTIFY13 showed significantly higher expression in reproductive organs than in vegetative organs, while LcTIFY11 exhibited significantly higher expression in female flower buds and ovaries than in male flower buds. 【Conclusion】The TIFY family members LcTIFY2, LcTIFY7, LcTIFY11 and LcTIFY13 may play roles in reproductive growth and fruit development of L. cylindrica.
MA Hongbo , WANG Zhenying , ZHAO Haiyun , ZHANG Jianguo , ZENG Yanfei
2024, 44(11):1760-1768. DOI: 10.7606/j.issn.1000-4025.20240279
Abstract:Abstract [Objective] To compare the genetic diversity and relationships between the chloroplast genomes of olive varieties selected and bred in China and those in the Mediterranean olives, and to estimate the maternal diversity of olive varieties selected and bred in China, and in turn provide a basis for the development of Chinese olive accession. [Methods] NOVOPlasty v.4.3.1 was used to assemble chloroplast genomes, which were based on the genome re-sequencing data of the nine olive varieties selected and bred in China and seven Iranian accessions, both of which were sequenced in this study, and fifty-two Mediterranean olive accessions downloaded from the database, and then calculated the sequence polymorphism; GPA, RAxML-NG, and Network 5.0 and other software were used to construct the chloroplast genome map, the relationships of phylogeny and network of haplotype respectively. [Results] The chloroplast genomes of the analyzed olive accessions were consistent in terms of tetrad structure and number of genes in this study. All chloroplast genomes identified seventeen haplotypes, among which, the olive varieties selected and bred in China have only one haplotype which share with the most cultivated olives. The oleasters have the highest genetic diversity, followed by the cultivated olives and then Iranian accessions, while nine olive varieties selected and bred held all genetic diversity of 0. The phylogenetic relationship was consistent with the network of haplotype, one branch includes the most of the cultivated varieties, Iranian olive accessions and all olive varieties selected and bred in China. [Conclusion] The nine olive varieties selected and bred in China showed an extremely low diversity of maternal origin. It is necessary to select and breed the olive varieties adapted to China’s habitats from a more comprehensive lineage of olive accession in the later stages.
SHU Min , QING Yun , Aodungerile , HONG Yan , XIN Ying , Laxinamujila
2024, 44(11):1769-1779. DOI: 10.7606/j.issn.1000-4025.20240112
Abstract:【Objective】To investigate the response of C, N, P, and K distribution law in Xanthoceras sorbifolia seedlings to water and nitrogen addition, providing references for water-nitrogen environmental adaptation strategies and high-quality seedling cultivation.【Methods】A two-factor (nitrogen and water) randomized block design was adopted in this study. four N fertilization levels [N0: g/(m2·a), N1: 5 g/(m2·a),N2:10 g/(m2·a) and N3:15 g/(m2·a)] and three levels of water addition [ well watered (WCK), drought (Wd), and fully watered(Ww)] were set up to explore the impact on the ecological stoichiometric characteristics of Xanthoceras sorbifolia seedlings" leaves, branches, and roots.【Results】(1) The C, N, P, and K levels of seedlings were independently or interactively influenced by nitrogen and water, while the stoichiometric ratios were mainly affected by nitrogen addition. Under well-watered conditions (WCK), nitrogen addition increased C, N, and P levels. Under drought conditions (Wd), high nitrogen addition (N3) Ncould resist drought by increasing K level and decreasing N and P levels. Under well-watered conditions (Ww), only high nitrogen addition (N3) effectively alleviated nitrogen limitation. (2) C, N, P, and K nutrients were mainly concentrated in leaves, supporting photosynthesis and vegetative growth. The variability of N and P contents in leaves was high , but the N/P ratio remained stable.(3) The same element exhibited a high correlation between different organs, particularly between leaves and roots, contributing to the maintenance of stable growth.【Conclusion】Nitrogen addition can alleviate nitrogen deprivation whereas increases the demand for P and K. It is recommended to reduce nitrogen fertilizer application under suitable water conditions to reduce pollution and maintain growth potential.
GAO Jinrong , REN Yan , LI Yimeng , HUANG Hengyu
2024, 44(11):1780-1788. DOI: 10.7606/j.issn.1000-4025.20240155
Abstract:[Objective] The study aims to explore the in vitro regeneration conditions of Codonopsis pilosula (Franch.) Nannf. and establish its artificial efficient rapid propagation system, to provide a theoretical basis for its seedling breeding. [Methods] The callus was induced by the stem tip and stem segment in different exogenous hormone combinations, and then the L16 (45) orthogonal experiment was carried out with coconut juice, 6-BA, NAA and KT to screen out the best explant and the differentiation and proliferation medium of callus-adventitious buds. Finally, the rooting and acclimatization of plantlets were realized outside the bottle. [Results] (1) The callus induction rate of stem segments in MS medium with 6-BA of 1.5 mg/L and NAA of 0.5 mg/L was up to 98.76%, while that of stem tips was only 76.56%. (2) Coconut juice had a significant effect on the proliferation of C. pilosula, and the proliferation coefficient reached 43.39 in MS medium with coconut juice of 60 mL/L, 6-BA of 1.5 mg/L, KT of 0.5 mg/L and NAA of 0.5 mg/L. (3) The rooting rate of regenerated buds in bottles was very low, but the regenerated buds rooting outside the bottle could form roots after 30 days, with a rooting rate of 87.96%, and the survival rate after transplanting was 97.73%. In addition, basal stem cluster buds and axillary buds appeared during callus induction and differentiation, which also greatly improved the proliferation coefficient of C. pilosula. [Conclusion] The stem segment was more suitable for callus induction, coconut juice promoted callus redifferentiation, and rooting outside the bottle significantly improved the rooting rate.
TANG Chenyu , ZHANG Bona , TANG Luyao , YE Linfeng , XIE Jiangbo , WANG Zhongyuan
2024, 44(11):1789-1800. DOI: 10.7606/j.issn.1000-4025.20240388
Abstract:【Objective】 Revealing the regional differentiation of common species Populus alba on different environmental adaptation strategies can provide data support for predicting plant adaptation potential under the background of climate change. 【Methods】 Nine state-owned forest farms were selected along the precipitation gradient from southeast to northwest China, and 18 functional traits related to water and carbon of the common species Populus alba were measured. The methods of trait network and principal component analysis were used to quantify the regional differentiation of traits, and to clarify the main climatic factors driving the variation of traits and their habitat adaptation strategies. 【Results】 (1) The average coefficient of variation of each trait was 12.09%-60.35%, and the variation level of photosynthetic physiology and stomatal related traits was generally higher than that of other traits. (2) The importance percentage of photosyntetically active radiation, growing season mean temperature, annual mean precipitation and aridity index on trait variation were 35.45%, 28.37%, 19.36% and 16.93%, respectively. Among them, photosyntetically active radiation in the growing season was the most important climatic factor driving trait variation. (3) The central trait of the trait network was the maximum net photosynthetic rate.【Conclusion】 There were differences in the adaptation strategies of P.alba in the precipitation gradient. From the humid/semi-humid region to the arid region, its carbon economy strategy changed from a relatively conservative type to an acquisition type.
XIE Lele , WANG Xiaoli , WANG Yanlong , MA Yushou , ZHOU Xuanbo
2024, 44(11):1801-1810. DOI: 10.7606/j.issn.1000-4025.20240218
Abstract:[Objective] In order to provide data support and theoretical guidance for grassland restoration and biodiversity protection, the effects of grazing suspension in spring on the niche of Carex tibetikobresia meadow plants were explored. [Methods] Taking different grazing time as the environmental gradient, the importance value, aboveground biomass, niche breadth and overlap value of different economic groups of typical Carex tibetikobresia meadow in three-river source region were analyzed by using single factor analysis of variance and r software package (Pheatmap). [Results] Grazing for 50 days increased the important value of Cyperaceae (15.09%) and decreased the important value of poisonous weeds (50.09%);The aboveground biomass of different economic groups (except poisonous weeds) was significantly increased by 50 days, which increased by 220.99%, 69.73% and 139.37% respectively; Carex tibetikobresia S. R. Zhang、Helictotrichon tibeticum(Roshev) Holub(0.999); There are 55 pairs with (NO) greater than 0.990, accounting for 26.10% of the total, and 3 pairs with NO less than 0.75, accounting for 1.43% of the total.[Conclusion]The grazing moratorium in spring affected the important values and aboveground biomass of different economic groups in Carex tibetikobresia meadow. From the niche point of view, grazing suspension in spring is beneficial to the growth of fine forage and can effectively promote grassland restoration.
Yuerensa Abudilimu , ZHAO Wei , WANG Xiaowei , ZHANG Aiqin
2024, 44(11):1811-1819. DOI: 10.7606/j.issn.1000-4025.20240127
Abstract:[Objective] Elucidating the tripping mechanism and the role and status of flower in this process,are of great significant to explor the diverse pollen dispersal mechanism [Methods] The studies were carried out based on the field observation of tripping phenomenon, pollinator visiting behavior, the control experiment of pollen transfer and the anatomical observation of flower structure in Medicago sativa L. cv. Xin Mu No.4. [Results] The specialization structure of wings and keel edge are nested with each other and form the touch sensitive flower structure of alfalfa. The “sensitiveness” structure not only has high selectivity to pollinators, but also can manipulate pollinators" visiting behavior by changing the location of honey hole before and after tripping. Once the keel flap is tripped, about 90% of the pollen in the anther was dispersed, the gynostemium will not return to the original position, and the flower would close on the same day, or it will continue to open 5-6 d, which is a one-time pollen release mechanism. Tripping efficiency of insects, being not related to the insect"s size, determines the tripping contribution rate together with its visiting frequency. The insect"s second visiting did not increase the removing of residual pollen from anthers and pollen deposition on stigma. [Conclusion]The special flower structure and its regulation to pollinators" foraging behavior promoted the occurrence of tripping phenomenon, and benefits maximizing of the male fitness in Medicago sativa L. cv. XinMu No.4.
TIAN Xiaoling , TAO Piaopiao , HUANG chengling , ZHANG Changqin
2024, 44(11):1820-1827. DOI: 10.7606/j.issn.1000-4025.20240304
Abstract:[Objective] The purpose of this study is to explore the pollination biology and reproductive characteristics of Rhododendron excellens, providing a theoretical basis for the conservation of small populations.[Methodd] This study observed the reproductive characteristics of R. excellens by investigating its floral characteristics, pollinator and its associated visiting characteristics, fragrance of flowers and breeding system. [Results] (1) Unlike widespread Rhododendron species, pollen vitality of R. excellens was lower whereas the stigma vitality is strong, with a longer duration. (2) The main pollinating insect was Apis cerana in the daytime. The main volatile was 1,8-cineole which could attract moths for pollinating R. excellens at night. (3)The breeding system test indicated that R. excellens can set fruit through self-pollination, but the fruit set rate of self-pollination is lower than that of cross-pollination. Artificial supplementary pollination treatment can greatly improve the fruit set rate compared to natural control, indicating that there is pollen limitation in the seed formation process of R. excellens.[Conclusion] The flower morphology of R. excellens tends to avoid autonomous self pollination,Simultaneously, low pollen viability but sustained strong stigma receptivity may be a strategy for plants to adapt to lower pollination efficiency. The main pollinating insect was A. cerana in the daytime. However, moth pollination that occurred at night can not be excluded. Its breeding system is self-compatible and is evolving towards a facultative mixed mating system.
LIU Jiajia , LIN Yicen , MIAO Jiayun , LI Shibo , XU Tianrui , LIN Lianbing
2024, 44(11):1828-1830. DOI: 10.7606/j.issn.1000-4025.20240193
Abstract:[Objective] Gastrodia elata Bl. f. cyaneum L. B. Lin represents newly identified forma of Gastrodia that originated from Xiaocaoba Town, Zhaotong, Yunnan. Comprehensive documentation of its morphological characteristics, including color photographs, accompanies this discovery. [Methods] Meticulous dissection, morphological examination, and comparative analysis with established specimens and literature. [Results] Distinguished features that set G. elata f. cyaneum apart include an inverted egg-shaped tuber, the protruding, long, gray-green terminal bud, and the clear blue color of the floral axis, flowers, and capsules. [Conclusion] This research establishes G. elata f. cyaneum as a novel forma. The identification of the novel G. elata forma not only enriches the diversity of orchids, but also presents valuable resources for the breeding of G. elata.
WANG Fuqian , WANG Penghang , ZHANG Mengfan , SU Jing , Jiamei LI
2024, 44(11):1831-1836. DOI: 10.7606/j.issn.1000-4025.20240061
Abstract:[Objective] Corydalis , a large genus of Papaveraceae, is considered one of the most challenging groups in taxonomy. The classification of Corydalis in Henan remains unclear due to insufficient exploration of taxonomic characteristics and a lack of thorough revisions. [Methods] After reviewing the original literature and specimens from the Chinese Virtual Herbarium (CVH),we conducted field investigations, took photos and collected specimens. [Results] We confirmed three newly recorded species of Corydalis species in Henan Province, namely C. nanwutaishanensis Z.Y. Su & Lidén, C. ochotensis Turcz. and C. wilfordii Regel. We also verified that C. shaanxiensis Lidén ex C. Y. Wu, H. Chuang & Z. Y. Su and C. micrantha (Steph.) Pers. were misidentified as C. curviflora Maxim. and C. humosa Migo. in previous literature. In addition, we found a translation error in the label of C. pseudoincisa C.Y.Wu, Z.Y.Su & Lidén, which might not occur in Henan. [Conclusion] This study can be used to provide data for the study of biodiversity science in Henan and the revision of Flora of Henan.







