Drought is one of the most severe environmental factors that may impair growth and development of plant and decrease crop productivity.Thellungiella halophila,an Arabidopsis-related halophyte is an emerging model used for plant abiotic stress tolerance research.The proteome investigation of the response of drought stress in T.halophila leaf will advance the understanding on how plant adapt to drought stress.In this study,a proteomic study based on two-dimensional gel electrophoresis is performed in order to analyze the drought stress response of T.halophila.Totally,63 differentially displayed spots are determined,including 31 up-regulated,14 newly-appeared,15 down-regulated and 3 disappeared protein spots.Nine differentially displayed spots,including thioredoxin,ferritin-1 and lectin are identified by mass spectrometry analysis.Function classification analysis of the nine identified proteins reveals that the drought tolerance of T.halophila is achieved,at least partly,by enhancing ROS scavenging,adjusting energy metabolism and maintaining photosynthesis function.