The disease index,reactive oxygen species（H₂O₂ and O^-·₂）content and activities of CAT,SOD and POD were examined in tomato leaves treated with CaCl₂,EGTA and methyl jasmonate,and then inoculated with Botrytis cinerea.Results showed that:(1)Ca,MeJA and both combined treatment (MeJA+Ca) decreased the incidence rates of tomato seedlings by 32.5%,38.0% and 54.5% compared with control,while the treatment with MeJA+Ca caused a significant decrease of disease incidence by 32.6% and 15.3% as compared to the treatment with Ca or MeJA.MeJA+EGTA treatment was higher by 30.3% than that of MeJA treatment,lower by 13.1% than that of EGTA treatment.The Ca handling was less by 34.2% than that of EGTA treatment.(2)Compared with the control,Ca,MeJA and MeJA+Ca treatment promoted the accumulation of reactive oxygen species in leaves.The active oxygen content of MeJA+Ca treatment was higher than that of single Ca or MeJA treatment.The accumulation of reactive oxygen species were inhibited by MeJA+EGTA compared with MeJA,but higher than that of EGTA treatment.The active oxygen content of Ca treatment was higher than that of EGTA treatment.(3)The SOD,CAT,POD activities were increased significantly treated with Ca,MeJA and the highest was MeJA+Ca treatment.The treatment sprayed with MeJA+EGTA,however,reduced the antioxidant enzyme activities compared with MeJA,but enhanced compared with EGTA treatment.The antioxidant enzyme activities of Ca handling were higher than that of EGTA treatment.This suggested calcium could adjust resistance to B.cinerea induced by methyl jasmonate.This result is related with the role that calcium promoted methyl jasmonate-induced accumulation of reactive oxygen and antioxidant enzyme activities.