In this study,with Yunnan local landrace rice ‘SB70’ and Jinghong erect type of purple O.rufipogon as materials,a pair of specific primers were synthesized according to DNA sequence of WRKY45 gene reported in GenBank and used in PCR amplification of the genomic DNA of the material.A specific DNA fragment about 1.3 kb was obtained after PCR and then sequenced,the deduced amino acid contain a typical conserved WRKYGQK of WRKY protein by Blast analysis.It was found that the similarity of the fragment with different cultivar WRKY45 gene in GenBank is above 85% although there were some differences of nucleotide.The WRKY45 gene was constructed into plant expression vector pCAMBIA1300 with 6-phosphomannose isomerase gene (PMI) as selection marker gene.After PCR confirmation,recombinant clone was transformed to callus of rice cultivar ‘Yunzijing 41’ via agrobacterium tumefaciens system and 24 transgenic plantlets were obtained.