The glutenin and gliadin in the Aegilops tauschii×Secale cereale amphiploid with increasing genetic stability were analyzed by SDS-PAGE and A-PAGE methods.The results showed that 3 bands of the amphiploid were detected in the HMW region,where in the first and the third bands have the same size with the 5^t and 10^t subunits of the parent A.tauschii.The second band was a new glutenin band which could not be found from its two parents.2^r and 6.5^r subunits of S.cereale could not be found in the amphiploid.In the LMW region,4 and 2 bands were not observed in their corresponding amphiploid for S.cereale and A.tauschii,respectively.In the γ and β regions of gliadin,there were altogether 5 bands of S.cereale not detected in the amphiploid and only 1 band was missed for A.tauschii in the ω region.The above results indicated that great changes have taken place between the two parental genomes controlling storage proten in the amphiploid.Compared with that of A.tauschii,greater changes have experienced in the storage proteins controlled by S.cereale.The inheritance mode of storage protein genes in the amphiploid appeared nonadditive effects.