Thirty-two endophytic fungal strains were isolated from the roots,stems and leaves of wild Cymbidium goeringii by flat-panel separation.Agar block method was used to obtain the strain for antimicrobial activity screening.The results showed that four endophytic fungi (12.50% of the total strains isolated) had inhibitory activities to the test strains.We found a strong inhibitory activity of endophytic fungus.Morphological observation indicates that the strain belongs to Mycelia sterlia.