By analyzing Transcriptome (SRA020132) sequences of Salvia miltiorrhiza from Shangluo and using the techniques of PCR,a new 2oxoglutaratedependent dioxygenase gene was cloned from Salvia miltiorrhiza for the first time and named as Sm2ODD1 (GenBank accession number JN935923).Sm2ODD1 DNA consisted of 1 365 bp including 3 exons and 2 introns.The fulllength cDNA of Sm2ODD1 was 1 189 bp,containing a single 951 bp opening reading frame and encoding a 316 aminoacid residues.Bioinformatics analysis showed that Sm2ODD1 contained a 2oxoglutaratedependent dioxygenase superfamily containing “distorted jelly roll” domain and “HTD”,“HX” and “RYS” motifs to bind 2oxoglutarate and Fe²⁺.Quantitative RTPCR analysis revealed that the gene expressed in different organs.The expression in roots was observably higher than in flowers,stems and leaves.The gene could be induced by methyl jasmonate,GA₃ and ABA.The results indicated that the Sm2ODD1 might be involved in the biosynthesis of terpene in Salvia miltiorrhiza.