To verify monotypic genus Dodartia (formely Scrophulariaceae sensu lato) familial placement and evaluate phylogenetic relation between Dodartia and its relatives,we sampled Dodartia orientalis and its comprehensive and putative relatives,and conducted phylogenetic analyses including Maximum Parsimony and Bayesian inference of one nuclear DNA (ITS) region and four chloroplast DNA gene regions (trnLF,rps16,rbcL,rps2) individually and combined.The main result of the phylogenetic analyses indicate that Dodartia is sister to Lancea with the Bootstrap (BS) values=97%,Posterior Probability (PP) values=100% of combined four chloroplast DNA genes data;BS=99%,PP=100% of ITS data and BS=100%,PP=100% of combined four chloroplast DNA genes and ITS data.Dodartia,Lancea and Mazus form a maximum supported monophyletic clade with BS=100% and PP=100% of all the data.This relation was also corroborated by morphological data.Based on these data,we suggest that Dodartia should be included in Mazoideae (Phrymaceae),and the most closest relative of Dodartia is Lancea.Dodartia and Lancea maybe included in Mazus and relative to Mazus omeiensis or sister to Mazus.