In this experiment,a molecular marker sequencerelated amplified polymorphism (SRAP) was applied to determine the diversity and relationships among 29 banana (Musa spp.) cultivars.The results showed that 25 out of 64 primer combinations turned out to be polymorphic and 324 polymorphism bands were obtained.The genetic relationships were analyzed using unweighted pairgroup method of arithmetic average cluster analysis (UPGMA),and the genetic similarity coefficients were calculated among the 29 banana cultivars.The genetic relationship among cultivars was correlated to the origin region of cultivars,and all 29 cultivars could be clustered into two major clusters closely corresponded with the genome composition.The result was consistent with the morphology,agronomic trait classification.All results indicated that the amplification of SRAP markers is an efficient method for estimating genetic similarity among banana cultivars and providing a scientific basis for banana genetic and breeding research.