In order to ascertain the allelopathy activity of cucumber seeds during germination,we studied the allelopathy effects of cucumber seeds aqueous extract,cucumber seed germination,cucumber radicle exudates,cucumber bud exudates,decomposed cucumber seedlings and seedling aqueous extract by adopting laboratory petri dish seed germination bioassay,using 4 categories of vegetables as receptors.The results indicated that:(1)The cucumber seed aqueous extract had an inhibitory effect on germination of Chinese cabbage,radish,tomato and cucumber seeds which showed the cucumber seed contained a few allelochemicals.(2)After extracted seed interior allelochemicals,the cucumber seed germination had allelopathy inhibitory effect on interplanted Chinese cabbage,radish and tomato seeds germination,and the cucumber radicle and bud exudates had different extent allelopathy inhibitory effect on after cultured Chinese cabbage,radish,tomato and cucumber seeds germination.The germination and growth of all vegetable receptors were inhibited to different extent with the different weight decomposed cucumber seedlings and different concentration seedling aqueous extract.It showed the concentration effect which was germination indexes,response index (RI) and synthesis effect (SE) value of vegetable receptors could decrease along with increasing of the weight of decomposed cucumber seedlings and the concentration of seedling aqueous extract.(3)The experiment of cucumber as receptor showed the allelochemicals of cucumber seeds aqueous extract and cucumber seedling organs had the autotoxicity on cucumber seed germination and growth,in which the autotoxicity of decomposed cucumber seedlings,seedling aqueous extract,cucumber radicle exudates and cucumber bud exudates were maximum.The studies suggested that allelochemicals of cucumber seeds aqueous extract,germination periods and seedling organs main inhibited the radicle growth,i.e.the radicle of vegetable receptors was most sensitive to allelopathy.Because release allelochemicals ways were different during cucumber seed germination,and the allelopathy response of Chinese cabbage,radish,tomato and cucumber were different.This experiment indicated during the early of cucumber germination and seedling growth,the allelochemicals began to compose and accumulate which released to environment by the radicle and the seedling and by seedling decomposition,then inhibited the germination and growth of receptor vegetable seeds.