The Chromosome numbers and karyotypes of six species of Saussurea DC.in the Hengduan Mountains were analyzed with technology of the conventional squashing slides.The karyotype formulas and types were as follow:S.polycolea var.acutisquama,2n=2x=32=20m+12sm,karyotype belonged to stebbins 2B type;S.globosa,2n=2x=34=16m+18sm,karyotype belonged to stebbins 2B type;S.katochaete,2n=2x=32=8m+18sm+6st,karyotype belonged to stebbins 3B type;S.columnaris,2n=2x=32=24m+8sm,karyotype belonged to stebbins 2B type;S.graminea,2n=2x=28=8m+18sm+2st,karyotype belonged to stebbins 3B type;S.hieracioides,2n=2x=32=12m+16sm+4st,karyotype belongs to stebbins 2B type.No satellites have been found in these six species.The chromosome number of S.polycolea var.acutisquama and S.columnaris is reported for the first time.