An alien disomic addition line,‘Shannong 120211’,which derived from the progeny of Thinopyrum intermedium and common wheat cv.‘Yannong15’,were identified in morphology,cytology,genome in situ hybridization(GISH),powdery mildew resistance and salt tolerance.Resistance identification of adult in field and seeding in lab showed that ‘Shannong 120211’ was nearly immune to powdery mildew.Compared with the ‘Shanrong 3’,which was highly resistant to salt,the result of salt tolerance is level 2.The results demonstrated that the main morphological traits of ‘Shannong 120211’,with the chromosome number of 2n=44 in root tip cells and chromosome configuration of 2n=22Ⅱat PMC MI.The results of GISH showed that there were two chromosomes of ‘Shannong 120211’ with obvious hybridization signals,which further proved that ‘Shannong 120211’ was an alien disomic addition line with a pair of Th.intermedium chromosomes in wheat background.Using 71 pairs chromosome specific molecular markers of group E to analyze the ‘Shannong120211’,we found marker BE494262 could reveal the specific band of Th.intermedium and be used to identify ‘Shannong 120211’.Using Thinopyrum elongatum and the addition line of Cs-Thinopyrum elongata(1E^e-7E^e) to further located the BE494262 at 2E^e,which identified that the added chromosomes of ‘Shannong 120211’ came from the 2E^e of T.intermedium.