Clone and Expression Analysis of HcDmc1 Gene from Halostachys caspica
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    Abstract:

    According to the transcriptome of Halostachys caspica under salt stress,we isolated an cDNA fragment from H.caspica.The obtained cDNA was named as disrupted meiotic cDNAof H.caspica.Sequence analysis indicated that HcDmc1 contains an open reading frame (ORF) of 1 035 bp,which encodes 344 amino acids.Conserved domain analysis showed that HcDmc1 has a conserved domain of RecA protein family.Phylogenetic tree analysis indicated that HcDmc1 is an independent branch,which is an stable hydrophilic proteins sub-cellularly cytoplasm.Real time quantitative PCR results showed that the expression of HcDmc1 gene in branch after 100 mmol/L NaCl salt stress for 7d was rapidly up-regulated and reached the highest after 700 mmol/L,which was 6.58-fold of the control.While in roots the expression of HcDmc1 reached the highest level at 14 d and about 1.79-fold of the control.Base on the experimental results,the expression of HcDmc1 gene could be induced by salt stress.

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DU Chi, ZHANG Fuchun. Clone and Expression Analysis of HcDmc1 Gene from Halostachys caspica[J]. Acta Botanica Boreali-Occidentalia Sinica,2015,35(2):220-226

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  • Online: March 21,2015
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