The 135 melon interspecific population was derived from crossing the high sugar cultivated inbred lines‘0246’as female parent and low sugar wild inbred lines‘Y101’as male parent,which was used to construct a linkage map and analyze quantitative trait locus(QTLs) associated with sugar content correlated traits in melon.The content of fructose,sucrose and glucose was measured,respectively.Accumulative quantity of the three kinds of sugars was used as the total sugar content.These datas were used to analyze the QTLs associated with sugar content correlated traits in melon.The results showed that:(1)The map contained 14 linkage groups spanning 726.30 cM with the average markers interval of 12.74 cM.(2)Two QTLs associated with total sugar and fructose content were detected,named as Ts3.1 and Fru4.1.The 2 QTLs distributed in 3 and 4 linkage groups,respectively,and they were all positive additive effects,which could have addition effects on the accumulation of total sugar and fructose content,respectively.Their LOD values were 3.60 and 3.10 respectively.The 2 QTLs were able to explain 14.89% and 13.02% of total phenotypic variation.The results of this study have laid an important foundation for gene accurate location and sugar content gene cloning in melon.