To study the precise roles of cytosol GAPDH in plant response to salt stress,we obtained 1 381 bp HcGAPDH from Chenopodiaceae uhalophyte Halostachys caspica with RACE technology.The ORF(open reading frame) of which encode 314 amino acids.HcGAPDH gene displayed considerable homology with known members of the GAPDH protein family.The expression of HcGAPDH is upregulated by salt stress and abscisic acid treatment.Green fluorescent protein- tagged HcGAPDH was localized in cytosol of the transgenic Arabidopsis thalinana cells,and could transfer into nucleus under salt stress,which is independent of carbohydrate- mediated signaling.Our results implicate that HcGAPDH function in plant cells through modulating cytosol to nucleus-signaling cascade under salt stress.The study laid a certain foundation for elucidation of salt-tolerance mechanism of Halostachys caspica at molecular level.
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ZHANG Xia, ZHANG Hua, ZHANG Fuchun. Subcellular Localization and Expression of Glyceraldehyde-3-phosphate Dehydrogenase Gene from Halostachys caspica under Salt Stress[J]. Acta Botanica Boreali-Occidentalia Sinica,2015,35(7):1283-1288