We designed SCAR markers according to the markers linked to clubroot resistant gene in Chinese cabbage,in order to develop the new clubroot resistant materials of non-heading Chinese cabbage through marker-assisted selection (MAS).The results indicated that one pair primer among 8 showed polymorphism between parents of resistant and susceptible,and co-segregation with reported SSR marker and resistant characterization of F₂,suggested that it could be used to identify the clubroot resistance,named CRb-R-25.Based on the inter-subspecies hybrid,backcross and selection of CRb-R-25,TQ14-1-15 with clubroot resistance of non-heading Chinese cabbage was bred by introgression of resistance gene in Chinese cabbage.