Based on the association coefficient(AC),Ochai index(OI),Jaccard index(JI),Pearson index and χ²-test to analyze the interspecific associations of medicinal herbs,and the interspecific associations between medicinal herbs and common trees in Qiaoshan forest region,Ziwuling Mountains.The results indicated that:(1)the significant positive interspecific associations were showed between Pulsatilla chinensis and Sophora flavescens,Polygonatum sibiricum and Adenophora stricta.On the other hand,negative associations were found between Dioscorea nipponica and Cirsium setosum,Potentilla discolor,Rhaponticum uniflorum,Artemisia capillaris,Polygala tenuifolia,as well as between P.sibiricum and P.discolor,A.capillaris each other.(2)Robinia pseudoacacia and C.setosum,Quercus wutaishanica and Polygonatum odoratum and P.sibiricum,Betula platyphylla and D.nipponica showed significant positive associations,while Robinia pseudoacacia and D.nipponica and Polygonatum odoratum,Quercus wutaishanica and C.setosum and P.discolor and A.capillaris and S.flavescens,Quercus aliena and Agrimonia pilosa and A.capillaris,Betula platyphylla and Arisaema erubescens and A.capillaris,Pinus tabulaeformis and Leonurus artemisia,Juglans cathayensis and P.sibiricum showed significant negative associations.(3)Based on the results of interspecific associations,three ecological species groups of medicinal herbs in this area were delineated.The result may reflect the pattern of ecological and physiological of species and also provides a basis on resource development and utilization of the medicinal plants in this region.