A full-length cDNA encoding pyruvate kinase was cloned firstly by RT-PCR and RACE-PCR from Lonicera japonica Thunb.,designated LjPkc (GenBank accession number JQ621946.1).The length of coding sequence (CDS) is 1 533 bp,which codes for a protein with 510 amino acids.LjPkc displayed high sequence identity with previously cloned Pkc gene of Nicotiana sylvestris and was clustered close to Pkc family genes of dicotyledon plants.Subcellular localization analysis showed that LjPkc protein may play an important role in the chloroplasts or mitochondria.The LjPkc proteins mainly have alpha-helix and random coil by spatial structure analysis.qPCR analyses showed that LjPkc is widely expressed in stem,leaf and flower of L.japonica Thunb.,but the mRNA of LjPkc has the highest expression level in yellow flower.The research is not only to enrich the gene resource of Pkc gene family,but also lay a foundation to reveal the biology function of LjPkc in sugar metabolism.