The ectomycorrhizal fungus from Ziwuling forest region on the Loess Plateau was isolated by tissue isolation technique,identified based on ITS sequence,and the cultivation condition in vitro was further investigated to establish the basis of inoculum production.Result showed the isolated fungus from Ziwuling forest region was Handkea utriformis,which could form ectomycorrhiza with Pinus tabulaeformis.Using MMN medium,the optimum pH was 6.0 and temperature was 25 ℃.Growth rate of H.utriformis on MMN medium with sucrose was the fastest,followed by maltose and glucose,and the slowest growth rate was for lactose.This fungus did not grow on medium using chitin and sodium bicarbonate as carbon source.For organic nitrogen source,H.utriformis grow fastest on MMN medium with proline,followed by casein,and slowest with urea.For inorganic nitrogen source,H.utriformis grow fastest with diammonium hydrogen phosphate,followed by calcium nitrate,and slowest with ammonium molybdate.For the first time,this study reported the ectomycorrhizal fungus H.utriformis in the Ziwuling region on the Loess Plateau,and its optimum growth condition in vitro was preliminary confirmed.