Late embryogenesis abundant protein(LEA) are a suite of important family proteins found in organisms, which are generally involved in osmoregulation and are expressed abundantly when subjected to environmental stresses. In this study, we isolated a novel LEA3 gene TaDRLea32 from wheat‘Zhengyin No.1’. The full length of TaDRLea32 is 668 bp, whose ORF is 570 bp, encoding 189 amino acids, and consists of 9 conserved 11mer repeating motifs; Protein prediction shows that the protein is highly hydrophilic and located in the cytoplasm, with three phosphorylation sites and has no transmembrane domain or signal peptide; Secondary structure analysis shows that the main structure is alpha helix; Homology comparison and regenerating phylogenetic tree were carried out by MEGA5.1. Expression analyses revealed that TaDRLea32 gene was induced by drought, lowtemperature, highsalt stress and also moreover, can be induced by ABA treatment. These results suggested that TaDRLea32 is an ABAdependent LEA3 gene, participating in the response process of wheat under the abiotic stresses in different mechanisms and can lay a foundation for elucidating LEA3 gene protection mechanism under abiotic stresses in wheat.