Diurnal variations of photosynthesis and physiological and ecological factors of peach trees with different growth types were studied by LI6400 portable photosynthetic system in different seasons. The photosynthesis characteristics of them in three seasons and the photosynthetic differences between different growth types were studied in 2015. The results showed that: (1) Among the photosynthetic ability of different growth types, the dwarf type was the highest, followed by the common type, weeping type was the lowest; The photosynthetic ability of dwarf type, weeping type and broom peach showed the following variation: spring > summer > autumn, and the normal type for: spring > autumn > summer; in the same growth type, the photosynthetic ability of long branches is generally higher than middle branches and short branches. (2) Among different growth types, the WUE of common type was the highest, and that of broom type was the lowest; the diurnal variation of water use efficiency (WUE) was a W type curve in spring and a U type curve in summer, while the curve in autumn is a broken line. The WUE in spring was the highest, and the difference between summer and autumn was little. (3) The diurnal change of P_n showed a unimodal curve in spring and autumn, but it showed a bimodal curve in summer and has a “midday depression” at noon, which caused by stomatal limitation and nostomatal factors. (4) Throughout the growing seasons, the photosynthetic active radiation (PAR), stomatal conductance (G_s) and transpiration rate (T_r) were very important to the diurnal change of P_n among the physiological and ecological factors. There were some differences in photosynthetic efficiency of different growth types and that of same growth types in different seasons, which were influenced by the genotype and environmental factors.